جستجو و تعیین میزان ملامین در شیر خشک نوزادان به روش الایزا

محورهای موضوعی : علوم و صنایع غذایی

امیر شاکریان ¹ (دانشگاه آزاد اسلامی، واحد شهرکرد، دانشکده دامپزشکی، دانشیار گروه بهداشت مواد غذایی، شهرکرد، ایران.)
گلی صوابی اصفهانی ² (دانشگاه آزاد اسلامی، واحد شهرکرد، دانشکده کشاورزی، دانش آموخته مهندسی علوم و صنایع غذایی، شهرکرد، ایران)

تاریخ دریافت : 1390/12/12 تاریخ پذیرش : 1391/07/08 تاریخ انتشار : 1391/03/01

کلید واژه: الایزا, ملامین, شیرخشک نوزادان,

چکیده مقاله :

در سال 1391 تعداد 36 نمونه شیر خشک نوزادان با نشان های تجاری مختلف و تاریخ های تولید متفاوت از نقاط مختلف شهر اصفهان خریداری شد و با استفاده از روش غربال گریELISAجستجو و میزان ملامین در انواع شیر خشک نوزادان تعیین شد. بر اساس نتایج آزمایش ، در هیچ کدام از نمونه های شیر خشک نوزادان مقادیر بالاتر از حد تشخیص کیت µg/L)10) مشاهده نگردید. با توجه به نتایج به دست آمده در این بررسی، مصرف شیر خشک های نوزادان موجود در بازار اصفهان در سال جاری خطری از نظر آلودگی ملامین برای سلامتی نوزادان ندارد.

چکیده انگلیسی:

   Thirty-six samples of infant formula with different production dates and various brands were purchased from Isfahan city during 2012. The samples were assayed for the presence and quantity of melamine by ELISA screening method. According to the results, in any infant formula melamine contamination was observed above the detection limit of the kit (10 µg/L). Therefore, it was concluded that the infant formula at Isfahan retail is not considered a health hazard from the melamine contamination point of view.

منابع و مأخذ:

• Braekevelt, E., Lau, B. and Feng, S. (2011). Determination of melamine, ammeline, ammelide and cyanuric acid in infant formula purchased in Canada by liquid chromatography-tandom mass spectrometry. Food Additives and Contaminants, 28: 698-704.


• Brown, C.A., Jeong, K.S., Poppenga, R.H., Puschner, B., Miller, D.M., Ellis, A.E., Kang, K.I., Sum, S., Cistola, A.M. and Brown, S.A. (2007). Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007. Journal of Veterinary Diagnostic  Investigation, 19: 525-531.


• Chi Chen, W., San-Yuan, W. and Hsin-Ping, L. (2010). Identification of Melamine/Cyanuric Acid-Containing Nephrolithiasis by Infrared Spectroscopy. Jornal of Clinical Laboratory Analysis, 24: 92-99.


• Dobson, R.L., Motlagh, S., Quijano, M., Cambron, R.T., Baker, T.R., Pullen, A.M., Regg, B.T., Bigalow-Kern, A.S., Vennard, T., Fix, A., Reimschuessel, R., Overmann, G., Shan, Y. and Daston, G.P. (2008). Identification and characterization of toxicity of contaminants in pet food leading to an outbreak of renal toxicity in cats and dogs. Toxicological Sciences, 106(1): 251-262.


• Goscinny, S., Hanot, V., Halbardier, J.F., Michelet, J.Y. and Van Loco, J. (2010). Rapid analysis of melamine residue in milk, milk products, bakery goods and flour by ultra-performance liquid chromatography/tandem mass spectrometry: from food crisis to accreditation. Food Control, 22: 226-230.


• Hau, K.C., Kwan, T.H. and Li, P.K. (2009). Melamine Toxicity and the Kidney. Journal of the American Society of Nephrology, 20: 245-250.


• Jorma, L. (2009). The detection of melamine in milk products. Food Contamination, 42: 22-25.


• Lei, H., Shen, Y., Song, L., Yang, J., Chevallier, O.P., Haughey, S.A., Wang, H., Sun, Y. and Elliott, C.T.(2010).Hapten synthesis and antibody production for the development of a melamine immunoassay. Analytica Chimica Acta, 665(1):84-90.


• Li, X., Luo, P., Tang, S., Beier, RC., Wu, X., Yang, L., Li, Y. and Xiao, X.(2011).Development of an immunochromatographic strip test for rapid detection of melamine in raw milk, milk products and animal feed. Journal of Agricultural and Food Chemistry, 59(11): 6064-6070.


• Puschner, B., Poppenga, R.H., Lowenstine, L.J., Filigenzi, M.S. and Pesavento, P.A. (2007). Assessment of melamine and cyanuric acid toxicity in cats. Journal of Veterinary Diagnostic Investigation, 19(6): 616-624.


• Ruicheng, W., Ran, W. and Qingfei, Z. (2009). High-Performance Liquid Chromatographic Method for the Determination of Cyromazine and Melamine Residues in Milk and Pork. Chromatographic Science, 47: 581-584.


• Sheryl, A., Benjamin, P. and Lay, Y. (2010). Baseline levels of melamine in food items sold in Canada. Dairy products and soy-based dairy replacement products. Food Additives and Contaminants, 3: 135-139.


• Tran, B.N., Okoniewski, R., Storm, R., Jansing, R. and Aldous, K.M. (2010). Use of methanol for the  efficient extraction and analysis of melamine and cyanuric acid residues in dairy products and pet foods. Journal of Agricultural and Food Chemistry, 58: 101-107.


• Wang, Q., Haughey, S.A., Sun, Y.M., Eremin, S.A., Li, H.L., Xu, Z.L., Shen, Y.D. and Lei, H.T. (2011).Development of a fluorescence polarization immunoassay for the detection of melamine in milk and milk powder. Analytical and Bioanalytical Chemistry, 399(6): 2275-2284.


• World Health Organization. (2011). International experts limit melamine levels in food: New guidance to help improve food safety provided by UN food standards commission. 20100706.


• Yin, W., Liu, J., Zhang, T., Li, W., Liu, W., Meng, M., He, F., Wan, Y., Feng, C., Wang, S., Lu, X. and Xi, R. (2010). Preparation of monoclonal antibody for melamine and development of an indirect competitive ELISA for melamine detection in raw milk, milk powder, and animal feeds. Journal of Agricultural and Food Chemistry, 58(14): 8152-8157.


• Yu, H., Tao, Y., Chen, D., Wang, Y., Liu, Z., Pan, Y., Huang, L., Peng, D., Dai, M., Liu, Z. and Yuan, Z. (2010). Development of a high performance liquid chromatography method and liquid chromatograyphy tandem mass spectrometry method with pressurized liquid extraction for simultaneous quantification and confirmation of cyromazine, melamine and its metabolites in foods of animal origin. Analytica Chemical Acta, 682(1-2): 48-58.


• Zongyi, W., Liying, Z., Wenjun, Y. and Limin, G. (2010). Screening and determination of melamine residues in tissue and body fluid samples. Analytica Chimica Acta, 662: 69-70.