Phytochemical constituents found in Physalis peruviana L. leaf extracts and their ability to inhibit alpha-glucosidase and scavenge DPPH free radicals in vitro

محورهای موضوعی : Pharmacognosy

Félicien Kasali ¹ , Muhammad Ali ² , Jonans Tusiimire ³ , Mehreen Lateef ⁴ , Ruth Omole ⁵ , Justin Kadima ⁶ , Amon Agaba ⁷

1 - Pharm-Bio Technology and Traditional Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda|Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda|Department of Pharmacy, Faculty of Pharmaceutical Sciences and Public Health, Official University of Bukavu, PO Box 570 Bukavu, the Democratic Republic of Congo
2 - HEJ Research Institute of Chemistry, International Centre for Chemical & Biological Sciences, University of Karachi, Karachi-75270, Pakistan
3 - Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda
4 - Multi-Disciplinary Research Lab, Bahria University, Medical & Dental College, Karachi, Pakistan
5 - School of Chemistry and Material Science, Technical University of Kenya, P.O Box 52428- 00200, Nairobi, Kenya
6 - Department of Pharmacy, Faculty of Pharmaceutical Sciences and Public Health, Official University of Bukavu, PO Box 570 Bukavu, the Democratic Republic of Congo|Department of Pharmacology, School of Medicine and Pharmacy, University of Rwanda, PO Box 117 Huye, Rwanda Mbarara
7 - Department of Pharmacology and Therapeutics, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda

تاریخ دریافت : 1400/09/08 تاریخ پذیرش : 1400/12/09 تاریخ انتشار : 1401/02/11

کلید واژه: GC-FID, Hexane fraction, MS, Bio-compounds, <i>Physalis peruviana</i> L,

چکیده مقاله :

Physalis peruviana L. is widely used in folk medicines to manage diabetes. This study aimed to identify new chemical constituents and explore in vitro antioxidant ability and alpha-glucosidase inhibition activity to explain its antidiabetic mechanism. Methanolic extracts and hexane fractions were prepared and analyzed by CG-FID and GC-MS. In vitro antidiabetic activity was assessed on α-glucosidase inhibition and DPPH models. The phytochemical analysis detected 29 constituents, manly heptacosane (25) and hexadecanoic acid methyl ester (14). Aliphatic hydrocarbons and esters were identified as the main classes of secondary metabolites. Compared to the standard, the methanol-water fraction was the most potent fraction on α-glucosidase inhibition (IC50 32.5 ± 0.27 µg/mL). The antioxidant values (IC50) of two fractions, and the reference were quite close (44.5 ± 0.54; 49.5 ± 0.87; 44.2 ± 0.77 µg/mL). Almost 82.75% are recognized for the first time in the leaf hexane fractions of this plant. The observed antioxidant activity was found to be significantly notable.

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