Shelf Life Extension of Package’s Using Cupper/(Biopolymer nanocomposite) Produced by One-Step Process
محورهای موضوعی : food microbiologyS. Ebrahimiasl 1 , S. A. Younesi 2
1 - Assistant Professor of the Department of Nanotechnology, Ahar Branch, Islamic Azad University, Ahar, Iran.
2 - Assistant Professor of the Department of Chemistry Engineering, Ahar Branch, Islamic Azad University, Ahar, Iran.
کلید واژه: Active Packaging, Antibacterial, Biodegradable, Bionanocomposite, Chitosan,
چکیده مقاله :
ABSTRACT: The use of new compounds to increase the shelf life of more perishable foods based on nanoparticles and biodegradable polymers have been developed in packaging. Chitosan (Cts) is a natural biopolymer with excellent biodegradability and nontoxicity and antibacterial effect. In this research antibacterial copper nanoparticles (CuNPs) were incorporated in the biodegradable chitosan matrix for applications as packaging materials. The properties of Cu/Cts bionanocomposites (BNCs) were studied as a function of the CuNPs concentration. Surface morphology and elemental composition of the film was investigated by scanning electron microscopy attached to EDXRF. Mechanical analysis and water vapor barrier properties of CuNPs/Cts composites were analyzed. It was observed that mechanical and water vapor barrier properties of the films were improved by the concentration of CuNPs. The antibacterial activity of CuNPs/Cts thin films were evaluated based on the diameter of the inhibition zone in a disk diffusion test against gram positive bacteria i.e., Staphylococcus aureus and Bacillus cereus and gram negative bacteria i.e., S.epidermidis and Streptococcus A. that are responsible in food deterioration, using Mueller Hinton agar at different concentration of CuNPs. The results revealed a greater bactericidal effectiveness for nanocomposite films containing 0.15mol/L of CuSO4 solution. Packages prepared from Cts/Cu nanocomposite films were used for meat packaging. The films were filled with meat and then stored at 4 ºC. Microbial stability of the meat was evaluated after 3, 7, 10 and 15 days of storage. The results showed that microbial growth rate significantly reduced as a result of using this nanocomposite packaging material.Keywords: Active Packaging, Antibacteria
Angles, M. N. & Dufresene, A. (2000). Plastisized Starch/Tunicine Whiskers nanocomposite 1. Structural analysis.Macromolecules. 33, 8344-8353.
Chaudhry, Q., Scotte, M., Blackburn, J., Ross, B., Boxall, A., & Castle, L. (2008). Applications and implications of nanotechnologies for the food sector.Food Additives and Contaminants, 25(3), 241-258.
Darder, M., Colilla, M. & Ruiz-Hitzky, E. (2005). Chitosan-clay nanocomposites: Application as electrochemical sensors. Applied Clay Science, 28, 199–208.
Ebrahimiasl, S., Wan Yunus, W. M. D., Kassim, A. & Zainal, Z. (2010). Prediction of grain size, thickness and absorbance of nanocrystalline tin oxide thin film by Taguchi robust design. Solid State Sciences, 12, 1323-1327.
Ebrahimiasl, S., Zakaria, A., Kassim, A. & Basri, S. N. (2015). Novel conductive polypyrrole/zinc oxide/chitosan bionanocomposite: synthesis, characterization, antioxidant, and antibacterial activities. International Journal of Nanomedicine, 10, 217–227.
Faundez, G., Troncoso, M., Navarrete, P. & Figueroa, G. (2004). Antimicrobial activity of copper surfaces against suspensions of Salmonella enterica and campylobacter. BMC Microbiology, 4, 19–25.
Feng, Q. L., Wu, J., Chen, G. Q., Cui, F. Z., Kim, T. N. & Kim, J. O. (2000). A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of Biomedical Materials Research, 52 (4), 662-668.
Imran, M., El-Fahmy, S., Revol-Junelles, A. M. & Desobry, S. (2010). Cellulose derivative based active coatings: effects of nisin and plasticizer on physico-chemical and antimicrobial properties of hydroxypropyl methylcellulose films. Carbohydrate Polymers, 81, 219–225.
Kassim, A., Block, H., Davis, F. J. & Mitchell, G. R. (1992). Anisotropic films of polypyrrole formed electrochemically using a non-planar dopant. Journal of Materials Chemistry, 2, 987-988.
Kawashita, M., Tsuneyama, S. & Mijaji, F. (2000). Antibacterial silver containing silica glass prepared by the sol-gel method.Biomaterials, 21, 393–398.
Kim, J. S., Kuk, E., Yu, K., Kim, J. H., Park, S. J. & Lee, S. J. (2007). Antimicrobial effects of silver nanoparticles.Nanomedicine: Nanotechnology, Biology, and Medicine, 3, 95-101.
Larsen, G. & Noriega, S. (2004). Dendrimer-mediated formation of Cu- CuOx nanoparticles on silica and their physical and catalytic characterization. Applied Catalysis A: General, 278, 73–81.
Lee, D., Cohen, R. E. & Rubner, M. F. (2005). Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. Langmuir, 21, 9651–9659.
Lok, C. N., Ho, C. M., Chen, R., He, Q. Y., Yu, W. Y. & Sun H. (2006). Proteomic analysis of the mode of antibacterial action of silver nanoparticles. Journal of Proteome Research, 5, 916-924.
Mary, G., Bajpai, S. K. & Chand, N. (2009). Copper alginate-cotton cellulose (CACC) fibers with excellent antibacterial properties. Journal of Engineered Fibers and Fabrics, 4, 24–35.
Mitchell, G. R. & Geri, A. (1987). Molecular Organisation of Electrochemically Prepared Conducting Polypyrrole Films. Journal of Physics D: Applied Physics, 20, 1346-1353.
NasimulAlam, S. & Mishra, M. K. (2013). SEM and EDX study of intermetallics in a copper-tin system and the oxidation behaviour of tin. Microscopy and Analysis, 7-11.
Ninnemann, K. W. (1968). Measurements of physical properties of flexible films. In: Sweeting, O. J. (Ed.), Science and Technology of Polymer Films. Interscience, London, England, pp: 546–649.
Patel, M. K., Nagare, B. J., Bagul, D.M., Haram, S. K. & Kothari D. C. (2005). Controlled synthesis of Cu nanoparticles in fused silica and BK7glasses using ion bean induced defects. Surface and Coatings Technology, 196, 96–99.
Ramteke, C., Chakraburti, T., KetanSarangi, B. & Pandey, R. A. (2013). Synthesis of silver nanoparticles from the aqueous extract of leave of Ocimum sanctum for enhanced antibacterial activity. Journal of chemistry, 7, 6603-66015.
Saniusman, M., Ezzat El Zowalaty, M., Shameli, K., Zainuddin, N., Salama, M. & Ibrahim N. A. (2013). Synthesis, characterization and antimicrobial properties of copper nanoparticles. International Journal of Nanomedicine, 8, 4467–4479.
Sawai, J. (2003). Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. Journal of Microbiological Methods, 54, 177 182.
Singh, P. & Nanda A. (2013). Antimicrobial and antifungal potential of zinc oxide nanoparticles in comparison to conventional zinc oxide particles. Journal of Chemical and Pharmaceutical Research, 5 (11), 457-463.
Siva Kumar, V., Nagaraja, B. M., Shashikala, V., Padmasri, A. H., Madhavendra, S. S. & Raju, B. D. (2004). microorganisms in water. Journal of Molecular Catalysis A: Chemical, 223, 313–319.
Tilaki, R. M., IrajiZad, A. & Mahadavi, S. M. (2007). Size, composition and optical properties of copper nanoparticles prepared by laser ablation in liquids. ApplPhys A: Journal of Molecular Catalysis A: Chemical, 88, 415–4192.
Wang, H., Huang, Y., Tan, Z. & Hu, X. (2004). Fabrication and characterization of copper nanoparticles thin-films and the electrocatalytic behavior. Analytica Chimica Acta. 526, 13–17.
Yoon, K., Byeon, J. H., Park, J. & Hwang, J. (2007). Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. Science of the Total Environment, 373, 572–5.
Zhu, H., Zhang C. & Yin, Y. (2005). Novel synthesis of copper nanoparticles: influence of the synthesis conditions on the particle size. Nanotechnology, 16, 3079–3083.
