اثرات ضد باکتریایی مارگاتوکسین بر جدایه های بالینی اسینتوباکتر بومانی مقاوم به سیپروفلوکساسین
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
سحر پور اصغر
1
(دانشجوی دکتری)
نجمه رنجی
2
(عضو هیات علمی)
لیلا اسدپور
3
(Department of Biology, Faculty of Science, Rasht Branch, Islamic Azad University, Rasht, Iran)
مهدی شهریاری نور
4
(گروه میکروبیولوژی، دانشکده علوم پایه، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران)
کلید واژه: اسینتوباکتر بومانی, سیپروفلوکساسین , MgTX , اثر سینرژیک,
چکیده مقاله :
چکیده:
مقدمه: اسینتوباکتر بومانی، یک پاتوژن گرم منفی، به عنوان یک پاتوژن فرصت طلب مرتبط با مراقبت های بهداشتی شناخته می شود. در این مطالعه، خواص ضد باکتریایی مارگاتوکسین (MgTX) در شرایط آزمایشگاهی بر روی جدایه‌های بالینی مورد بررسی قرار گرفت.
روشها : پروفایل مقاومت دارویی از پنج آنتی بیوتیک: پیپراسیلین (100 میکروگرم)، ایمی پنم (10 میکروگرم)، سفتازیدیم (30 میکروگرم)، آمیکاسین (30 میکروگرم)، و سیپروفلوکساسین (5 میکروگرم) به روش انتشار از دیسک (Kirby-Bauer) در 20 جدایه بالینی مورد ارزیابی قرار گرفت. تشکیل بیوفیلم با استفاده از روش میکروتیتر پلیت ارزیابی شد. اثر سینرژیک MgTX در ترکیب با سیپروفلوکساسین مرود ارزیابی قرار گرفت.
یافته ها: نتایج ما نشان داد که مقاومت به همه آنتی بیوتیکها ≥90% بود. همه جدایه ها توان تشکیل بیوفیلم (متوسط تا قوی) را داتشند. روش چکر بورد، اثر سینرژیک بین از MgTX و سیپروفلوکساسین را تایید کرد.
نتیجه گیری: به نظر می رسد MgTX با خواص ضد باکتریایی بتواند در استراتژی های درمانی در آینده علیه جدایه‌های مقاوم به آنتی بیوتیک مورد استفاده قرار گیرد
کلیدواژه ها: اسینتوباکتر بومانی، سیپروفلوکساسین ، MgTX ، اثر هم افزایی
Introduction: Acinetobacter baumannii, a gram-negative pathogen, is recognized as a healthcare-associated opportunistic pathogens. In this study, the in vitro antibacterial properties of margatoxin (MgTX) were investigated against clinical isolates.
Method: The drug resistance profile of five antibiotics: piperacillin (100μg), imipenem (10μg), ceftazidime (30μg), amikacin (30μg), and ciprofloxacin (5μg) were evaluated with disc diffusion (Kirby-Bauer) method in 20 clinical isolates. Biofilm formation was assessed using the microtiter plate assay. Synergistic effects of MgTX in combination with ciprofloxacin was evaluated.
Results: Our results showed that the resistance to all antibiotics was ≥90%. All isolates had ability of biofilm formation (moderate to strong). Checker board method confirmed synergistic effect between MgTX and ciprofloxacin. indicated that a dose-dependent suppression of bacterial growth was achieved with the combined use of MgTX and ciprofloxacin.
ِِDiscussion: It seems that MgTX, with antibacterial properties, can use in therapeutic strategies in future against antibiotic resistant isolates.
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