Antibacterial Effects of Margatoxin on Ciprofloxacin Resistant Clinical Isolates of Acinetobacter baumannii
الموضوعات : Biotechnological Journal of Environmental Microbiology
سحر پور اصغر
1
,
نجمه رنجی
2
,
لیلا اسدپور
3
,
مهدی شهریاری نور
4
1 - گروه زیست شناسی ، دانشکده علوم پایه، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
2 - گروه زیست شناسی ، دانشکده علوم پایه، واحد رشت ، دانشگاه آزاد اسلامی ، رشت ، ایران
3 - گروه زیست شناسی ، دانشکده علوم پایه، واحد رشت، دانشگاه آزاد اسلامی ، رشت ، ایران
4 - گروه زیست شناسی، دانشکده علوم پایه، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
الکلمات المفتاحية: Acinetobacter baumannii, ciprofloxacin, MgTX, Synergistic effect,
ملخص المقالة :
Acinetobacter baumannii, a gram-negative pathogen, is recognized as healthcare-associated opportunistic
pathogens. In this study, the in vitro antibacterial properties of margatoxin (MgTX) were investigated against
clinical isolates. The drug resistance profile of five antibiotics: piperacillin (100μg), imipenem (10μg), ceftazidime
(30μg), amikacin (30μg), and ciprofloxacin (5μg) were evaluated with disc diffusion (Kirby-Bauer)
method in 20 clinical isolates. Biofilm formation was assessed using the microtiter plate assay. Synergistic
effects of MgTX in combination with ciprofloxacin was evaluated. Our results showed that the resistance to all
antibiotics was ≥90%. All isolates were resistant to ciprofloxacin (128-1024 μg/mL). Additionally, all isolates
had ability of biofilm formation (moderate to strong). Checkerboard method confirmed synergistic effect between
MgTX and ciprofloxacin. indicated that a dose-dependent suppression of bacterial growth was achieved
with the combined use of MgTX and ciprofloxacin. It seems that MgTX, with antibacterial properties, can use
in therapeutic strategies in future against antibiotic resistant isolates.
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