Antibacterial effects of margatoxin on ciprofloxacin resistant clinical isolates of Acinetobacter baumannii

Subject Areas : Biotechnological Journal of Environmental Microbiology

Sahar Pourasgar ¹ , Najmeh Ranji ² , Leila Asadpour ³ , Mahdi Shahriarinour ⁴

1 - Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran.
2 - Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
3 - Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
4 - Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

Received: 2023-09-18 Accepted : 2023-10-17 Published : 2023-10-21

Keywords: Acinetobacter baumannii, ciprofloxacin, MgTX, Synergistic effect ,

Abstract :

Introduction: Acinetobacter baumannii, a gram-negative pathogen, is recognized as a healthcare-associated opportunistic pathogens. In this study, the in vitro antibacterial properties of margatoxin (MgTX) were investigated against clinical isolates.

Method: The drug resistance profile of five antibiotics: piperacillin (100μg), imipenem (10μg), ceftazidime (30μg), amikacin (30μg), and ciprofloxacin (5μg) were evaluated with disc diffusion (Kirby-Bauer) method in 20 clinical isolates. Biofilm formation was assessed using the microtiter plate assay. Synergistic effects of MgTX in combination with ciprofloxacin was evaluated.

Results: Our results showed that the resistance to all antibiotics was ≥90%. All isolates had ability of biofilm formation (moderate to strong). Checker board method confirmed synergistic effect between MgTX and ciprofloxacin. indicated that a dose-dependent suppression of bacterial growth was achieved with the combined use of MgTX and ciprofloxacin.

ِِDiscussion: It seems that MgTX, with antibacterial properties, can use in therapeutic strategies in future against antibiotic resistant isolates.

References:

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