Isolation and Identification of Coagulase Positive Staphylococcus aureus in Cow Row Milk in Tehran by Culture and Real-Time PCR Methods
محورهای موضوعی : Food and Health
Jalal Shayegh
1
,
Sahba Mohamadzadeh
2
1 - Veterinary Medicine College, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - Veterinary Medicine College, Shabestar Branch, Islamic Azad University, Shabestar, Iran
کلید واژه: Coagulase positive, Staphylococcus aureus, raw milk, Tehran, real-time PCR,
چکیده مقاله :
Coagulase-positive Staphylococcus aureus (S. aureus) is one of the main causes of contamination of raw milk and dairy products, which can lead to food poisoning in humans. The rapid and accurate identification of this bacterium is of great importance. The aim of this study was to investigate the contamination of raw milk from Tehran cattle farms with S. aureus using culture and real-time PCR methods. For this study, 60 raw milk samples were collected from different cattle farms in Tehran. The samples were first analyzed using the culture method on Baired-Parker agar medium. The colonies with black centers and light areas were used for the coagulase test with rabbit plasma. The real-time PCR technique and the transcriptional regulator gene (Sa0836) specifically developed for S. aureus were then used to confirm the results. Of the 60 raw milk samples, 40 samples (66.66%) were identified as Staphylococcus in the culture medium. Of these, 32 samples (53.33%) were positive in the coagulase test. The results of the molecular tests also showed that S. aureus infection was confirmed in 27 samples (45%). The results of this study show that the contamination of raw milk in Tehran is significant and that the use of culture methods as a gold standard together with molecular techniques such as real-time PCR can help to increase the speed and accuracy of detection of S. aureus in raw milk. This approach can help to improve the quality and safety of dairy products and maintain public health.
Coagulase-positive Staphylococcus aureus (S. aureus) is one of the main causes of contamination of raw milk and dairy products, which can lead to food poisoning in humans. The rapid and accurate identification of this bacterium is of great importance. The aim of this study was to investigate the contamination of raw milk from Tehran cattle farms with S. aureus using culture and real-time PCR methods. For this study, 60 raw milk samples were collected from different cattle farms in Tehran. The samples were first analyzed using the culture method on Baired-Parker agar medium. The colonies with black centers and light areas were used for the coagulase test with rabbit plasma. The real-time PCR technique and the transcriptional regulator gene (Sa0836) specifically developed for S. aureus were then used to confirm the results. Of the 60 raw milk samples, 40 samples (66.66%) were identified as Staphylococcus in the culture medium. Of these, 32 samples (53.33%) were positive in the coagulase test. The results of the molecular tests also showed that S. aureus infection was confirmed in 27 samples (45%). The results of this study show that the contamination of raw milk in Tehran is significant and that the use of culture methods as a gold standard together with molecular techniques such as real-time PCR can help to increase the speed and accuracy of detection of S. aureus in raw milk. This approach can help to improve the quality and safety of dairy products and maintain public health.
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