ارزیابی ترکیبات فنولی موجود در اندام هوایی و ریشه کاسنی (.Cichorium intybus L) جمع آوری شده از مناطق مختلف کشور با استفاده از دستگاه کروماتوگرافی مایع با کارآیی بالا (HPLC)
محورهای موضوعی : فیتوشیمیمهشید رحیمی فرد 1 , فاطمه سفیدکن 2 , سمیه فکری قمی 3 , مریم مکی زاده تفتی 4
1 - عضو هیات علمی موسسه تحقیقات جنگلها و مراتع کشور
2 - عضو هیات علمی موسسه تحقیقات
3 - موسسه تحقیقات جنگلها و مراتع کشور
4 - موسسه تحقیقات جنگلها و مراتع ایران، بخش گیاهان دارویی
کلید واژه: اولتراسونیک, عصاره, شیکوریک اسید, کاسنی, کلروژنیک اسید,
چکیده مقاله :
در این مقاله بررسی فیتوشیمیایی شامل شناسایی و اندازه‌گیری برخی ترکیبات فنولی موجود در عصاره‌های هیدروالکلی گیاه کاسنی که از 6 منطقه از کشور برداشت شده بود، انجام شد. از بین ترکیبات مورد مطالعه شیکوریک اسید (با مقادیر 55/0 تا 04/6 میلی¬گرم به ازای هر گرم گیاه خشک)، کلروژنیک اسید (با مقادیر 50/0 تا 37/5 میلی¬گرم به ازای هر گرم گیاه خشک)، و روتین (با مقادیر 56/0 تا 25/2 میلی¬گرم به ازای هر گرم گیاه خشک) در عصاره¬های اندام هوایی و ترکیبات شیکوریک اسید (با مقادیر 03/0درصد تا 29/0 میلی¬گرم به ازای هر گرم گیاه خشک) و کلروژنیک اسید (با مقادیر 84/0درصد تا 88/2 میلی¬گرم به ازای هر گرم گیاه خشک) در ریشه¬ همه نمونه¬ها یافت شد. در بین جمعیت¬های مورد مطالعه بیشترین میزان ماده موثره شیکوریک اسید و کلروژنیک اسید در اندام‌های هوایی مربوط به نمونه جمع آوری شده از باغ ملی گیاه شناسی ایران (توده زراعی) با مقادیر 04/6 میلی¬گرم شیکوریک اسید و 37/5 میلی¬گرم کلروژنیک اسید به ازای هر گرم گیاه خشک مشاهده شد.
In this article, a phytochemical investigation including identification and measurement of some phenolic compounds in the hydroalcoholic extracts of the Cichorium intybus L. collected from 6 localities was carried out. Among the studied compounds, chicoric acid (0.55 to 6.04 mg per gram of dry plant), chlorogenic acid (0.50 to 5.37 mg per gram of dry plant), and rutin (0.56 to 2.25 mg per gram of dry plant) were found in extracts of aerial parts and chicoric acid (0.03% to 0.29 mg per gram of dry plant) and chlorogenic acid (0.84% to 2.88 mg per gram of dry plant) were found in the roots of all samples. Among the studied populations, the highest amount of major compounds (chicoric acid and chlorogenic acid) were observed in the aerial parts of the sample collected from the National Botanical Garden of Iran with 6.04 mg of chicoric acid and 5.37 mg chlorogenic acid per gram of dry plant.
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