بررسی سرولوژیکی توکسوپلاسما گوندی در بزهای مرغز ماده سقز، کردستان

محورهای موضوعی : پژوهش های بالینی دام های بزرگ

غزاله ادهمی ¹ (Iran-kurdistan-sanandai-shebli bulevard-danesh 1 avenue-22 plaque-)
آرام پرویزی ² (دانش آموخته دکترای حرفه ای، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران)

تاریخ دریافت : 1399/12/14 تاریخ پذیرش : 1400/08/25 تاریخ انتشار : 1400/09/01

کلید واژه: الایزا, سقز, شیوع, کردستان, توکسوپلاسما گوندی, بز مرغز,

چکیده مقاله :

زمینه و هدف: توکسوپلاسما گوندی یکی از انگل‌های تک یاخته ای مشترک منتقله از گوشت می‌باشد که موجب عفونت مادرزادی، سقط جنین ومرده زائی در انسان و حیوانات می‌شود. بز یکی از مهمترین منابع تولیدات گوشتی در استان کردستان می‌باشد و مصرف گوشت خام یا نیم پخته یکی از مهمترین ریسک فاکتورهای ابتلا به عفونت توکسوپلاسمایی می‌باشد. هدف از مطالعه حاضر، بررسی شیوع سرمی آلودگی به توکسوپلاسما گوندی در بزهای مرغز ماده سقز در استان کردستان در غرب ایران می‌باشد.مواد و روش ها: دریک مطالعه مقطعی 188 نمونه سرمی (144 نمونه سرم متعلق به بزهای با سابقه سقط جنین و 44 نمونه متعلق به بزهای بدون سابقه سقط جنین ) جهت ارزیابی آنتی بادی‌ های توکسوپلاسما گوندی به روش الایزا به کار گرفته شد.نتایج: از مجموع 188 سرم بررسی شده در این مطالعه 38 سرم با استفاده از تکنیک الایزا از نظرآلودگی به توکسوپلاسما گوندی مثبت تشخیص داده شدند که در مجموع میزان آلودگی بزهای مورد مطالعه 2/20 درصد بود. از مجموع 56 بز مرغز زیر 2 سال، 2 مورد (57/3%) ازنظر آلودگی به توکسوپلاسما وازمجموع 132 بز مرغز بالای 2 سال، 36 مورد (27/27%) مثبت بودند. دربررسی‌های آماری ارتباط معنی‌داری بین میزان آلودگی وگروه‌های سنی دیده شد (05/0p<). از مجموع 144 بز مرغز باسابقه سقط جنین، 36 مورد (25%) از نظر آلودگی به توکسوپلاسما و از مجموع 44 بز مرغز بدون سابقه سقط جنین، 2 مورد (54/4%) مثبت بودند. در بررسی‌های آماری همچنین ارتباط معنی‌داری بین میزان آلودگی و سقط جنین دیده شد(05/0p<).بحث و نتیجه گیری: باتوجه به شیوع توکسوپلاسما در بزهای مرغز اطلاع‌رسانی در راستای پیشگیری و کنترل و نیز خودداری از مصرف گوشت نپخته یا کم پخته شده درکنار مطالعات مولکولی توصیه می‌شود.

چکیده انگلیسی:

Background & Purpose: Toxoplasma gondii is a meat-borne zoonotic parasitic protozoan that causes congenital infection, miscarriage, and stillbirth in the human and animals. Goat is an important resource of meat production in Kurdistan Province, and the consumption of its raw or half-cooked meat is a major risk factor for infection with Toxoplasma. The present study aimed to investigate the seroprevalence of Toxoplasma gondii infection in Markhoz goats of Saqqez City of Kurdistan province in western Iran.Materials & Methods: In a cross-sectional study, we utilized 188 serum specimens ( 144 serum samples belonging to goats with a history of abortion and 44 samples belonging to goats without a history of abortion ) for the evaluation of Toxoplasma gondii antibodies by ELISA. 38 samples out of 188 serum specimens in the study were positive for Toxoplasma gondii using ELISA, and the infection rate of the goats was 20.2%.Results: Two (3.57%) out of 56 Markhoz goats under 2 years of age were positive for Toxoplasma infection and 36 (27.27%) out of 132 Markhoz goats over two years of age were positive for the infection. The statistical examination indicated significant relationships between the pollution and age groups (p<0.05). 36 (25%) out of 144 Markhoz goats with a history of abortion were positive for Toxoplasma infection, and 2 (4.54%) out of 44 goats without a history of abortion were positive for the infection. The statistical studies indicated a significant correlation between the rates of infection and abortion (p<0.05).Conclusion: Given the prevalence of Toxoplasma gondii in Markhoz goats, we suggest providing information about prevention and control, refraining from eating uncooked or undercooked meat, and performing molecular studies.

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