ارزیابی اثر مهارکنندگی عصاره‌های خیار دریایی (Holothuria parva) بر آنزیم آلفا آمیلاز

محورهای موضوعی : فصلنامه زیست شناسی جانوری

نجیبه قناعت پیشه ¹ (گروه زیست‌شناسی دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران)
موسی کشاورز ² (گروه زیست‌شناسی دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران)
حامد میر ³ (دانشگاه علوم پزشکی و خدمات بهداشتی درمانی جهرم، جهرم، ایران)

تاریخ دریافت : 1402/04/02 تاریخ پذیرش : 1402/05/09 تاریخ انتشار : 1402/12/01

کلید واژه: خیار دریایی, دیابت, خلیج‌فارس, خارپوستان, آکاربوز,

چکیده مقاله :

مهارکننده‌های آنزیم آلفا آمیلاز از تبدیل پلی‌ساکاریدها به مونوساکاریدها پیشگیری می‌کند. مهار این آنزیم از جذب قندهای ساده دستگاه گوارش و در نتیجه پیشگیری از افزایش سطح گلوکز خون موثر بوده است. هدف از مطالعه حاضر، بررسی تاثیر عصارهای اتیل استات، متانول و آب- متانول از خیار دریایی Holothuria parva بر مهار آنزیم آلفا آمیلاز است. در این پژوهش از پوسته و امعاء و احشاء گونه Holothuria parva ابتدا عصاره‌گیری براساس افزایش قطبیت انجام گرفت و در مرحله‌ بعد فعالیت آنزیم آلفا آمیلاز در حضور عصاره‌های مختلف تحت شرایط سنجش فعالیت آنزیمی به روش رنگ‌سنجی DNSA تعیین شد و IC50 (غلظتی از عصاره‌ها که برای برای مهار 50 درصد فعالیت آنزیم مورد نظر بوده است) بدست آمده و با مقدار آکاربوز به ‌عنوان کنترل مثبت مقایسه شد. در این بررسی نتایج نشان داد که با افزایش غلظت عصاره‌ها درصد مهارکنندگی افزایش یافته و در بین تمام عصاره‌ها بیشترین درصد مهارکنندگی مربوط به آکاربوز در غلظت 8/0 میلی‌گرم/میلی‌لیتر برابر 92/96 درصد و کمترین مقدار آن مربوط به عصاره آب–متانول پوسته در غلظت 2/0 میلی‌گرم/میلی‌لیتر برابر با 79/61 درصد بوده و درصد بازدارندگی آکاربوز در هر دو اجزای بدنی نسبت به سایر عصاره‌ها بالاتر است (IC50 برابر با 18/88- 74/91 میلی‌گرم/میلی‌لیتر). با توجه به اثر مهاری عصاره‌های استخراج شده از گونه Holothuria parva بر آنزیم آلفا آمیلاز در آینده‌ می‌توان در زمینه تولید داروهای ضددیابتی با منشا دریایی با حداقل عوارض جانبی نامطلوب استفاده نمود.

چکیده انگلیسی:

Alpha-amylase enzyme inhibitors prevent the conversion of polysaccharides into monosaccharides. Inhibiting this enzyme from the absorption of simple sugars in the digestive system and thus preventing the increase in blood glucose levels has been effective. The purpose of this study is to investigate the effect of ethyl acetate, methanol and water-methanol extracts of Holothuria parva on alpha-amylase enzyme inhibition. In this research, first extraction was done from the body wall, intestines and viscera of Holothuria parva based on increasing polarity, and in the next step, alpha-amylase enzyme activity was determined in the presence of different extracts under enzyme activity measurement conditions by DNSA colorimetric method, and IC50 were obtained and compared with the amount of acarbose as a positive control. In this study, the results showed that the percentage of inhibition increased with the increase in the concentration of the extracts and among all the extracts, the highest inhibitory percentage related to acarbose at a concentration of 0.8 mg/ml equals 96.92% and the lowest amount related to the water-methanol extract of the body wall at a concentration of 0.2 mg/ml equals 61.79% and the inhibition percentage of acarbose in both body parts is higher than other extracts (IC50=88.18-91.74 mg/ml). Considering the inhibitory effect of extracts extracted from Holothuria parva species on alpha-amylase enzyme, in the future, it can be used in the field of producing antidiabetic drugs of marine origin with minimal adverse side effects.

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