• Home
  • Inhibitory Effect of Sea Cucamber, Holothuria parva Extract on Activity of Alpha -Amylase

Share To

Article Url


Manuscript ID : ASCIJ-2306-1501 (R1) Visit : 237 Page: 57 - 70

10.22034/ascij.2023.1989599.1501

Article Type: Original Research

Inhibitory Effect of Sea Cucamber, Holothuria parva Extract on Activity of Alpha -Amylase

Subject Areas : Journal of Animal Biology

Najibe Ghenaat Pishe 1 , Mousa Keshavarz 2 , Hamed Mir 3

1 - Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran
2 - Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran
3 - Jahrom University of Medical Sciences and Health Services, Jahrom, Iran

Received: 2023-06-23 Accepted : 2023-07-31 Published : 2024-02-20

Keywords: Persian Gulf, Diabetes, Acarbose, Sea Cucamber, Echinodermat,

Abstract :

Alpha-amylase enzyme inhibitors prevent the conversion of polysaccharides into monosaccharides. Inhibiting this enzyme from the absorption of simple sugars in the digestive system and thus preventing the increase in blood glucose levels has been effective. The purpose of this study is to investigate the effect of ethyl acetate, methanol and water-methanol extracts of Holothuria parva on alpha-amylase enzyme inhibition. In this research, first extraction was done from the body wall, intestines and viscera of Holothuria parva based on increasing polarity, and in the next step, alpha-amylase enzyme activity was determined in the presence of different extracts under enzyme activity measurement conditions by DNSA colorimetric method, and IC50 were obtained and compared with the amount of acarbose as a positive control. In this study, the results showed that the percentage of inhibition increased with the increase in the concentration of the extracts and among all the extracts, the highest inhibitory percentage related to acarbose at a concentration of 0.8 mg/ml equals 96.92% and the lowest amount related to the water-methanol extract of the body wall at a concentration of 0.2 mg/ml equals 61.79% and the inhibition percentage of acarbose in both body parts is higher than other extracts (IC50=88.18-91.74 mg/ml). Considering the inhibitory effect of extracts extracted from Holothuria parva species on alpha-amylase enzyme, in the future, it can be used in the field of producing antidiabetic drugs of marine origin with minimal adverse side effects.

References:


  1. Abbasi H., Moein S., Ehsanpoor M. 2019. α-Glucosidase Inhibition and Antioxidant Activities of Respiratory Tree, Gonad, and Body Wall Extracts of Two Species of Sea Cucumbers (Holothuria leucospilota, Stichopus hermanni) from Persian Gulf: Bioactivities of respiratory tree extracts of two species of sea cucumbers. Trends in Peptide and Protein Sciences, 4:1-6 (e9).
    2.
  2. Abirami P., Arumugam M., Ajithkumar T.T., Balasubramanian T. 2011. Isolation and characterization of 37 kDa heparinase from the purple fluid of Dolabella auricularia. Indian Journal of Geo-Marine Sciences, 40(1):112-116.
    3.
  3. Althunibat O., Ridzwan B., Taher M., Daud J., Jauhari Arief Ichwan S., Qaralleh H. 2013. Antioxidant and cytotoxic properties of two sea cucumbers, Holothuria edulis Lesson and Stichopus horrensActa Biologica Hungarica, 64(1):10-20.
    4.
  4. Althunibat O.Y., Hashim R.B., Taher M., Daud J.M., Ikeda M.A., Zali B.I. 2009. In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species. European Journal of Scientific Research, 37 (3):376-387.
    5.
  5. Bruckner A.W., Johnson K.A., Field J.D. 2003. Conservation strategies for sea cucumbers: Can a CITES Appendix II listing promote sustainable international trade. SPC Bêche-de-mer Information Bulletin, 18(1):24-33.
    6.
  6. Burski K., Ueland T. Maciejewski R. 2004. Serum amylase activity disorders in the course of experimental diabetes in rabbits. Veterinarni Medicina, 49(6):197.
    7.
  7. Cao R.A., Surayot U. You S. 2017. Structural characterization of immunostimulating protein-sulfated fucan complex extracted from the body wall of a sea cucumber, Stichopus japonicus. International Journal of Biological Macromolecules, 99:539-548.
    8.
  8. Ceriello A., Davidson J., Hanefeld M., Leiter L., Monnier L., Owens D., Tajima N. Tuomilehto J. 2006. Postprandial hyperglycaemia and cardiovascular complications of diabetes: an update. Nutrition, Metabolism and Cardiovascular Diseases, 16(7):453-456.
    9.
  9. Coman C., Rugina O.D., Socaciu C. 2012. Plants and natural compounds with antidiabetic action. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1):314-325.
    10.
  10. Dabbagh A.R., Keshavarz M. 2011. three sea cucumbers from the Bandar-e Bosthaneh coast (Persian Gulf IRAN). World Applied Sciences Journal, 13(8):1933-1937.
    11.
  11. Debbab A., Aly A.H., Lin W.H., Proksch P. 2010. Bioactive compounds from marine bacteria and fungi. Microbial Biotechnology, 3(5):544-563.
    12.
  12. Duan J., Ishida M., Aida K., Tsuduki T., Zhang J., Manabe Y., Hirata T. Sugawara T. 2016. Dietary cerebroside from sea cucumber (Stichopus japonicus): absorption and effects on skin barrier and cecal short-chain fatty acids. Journal of Agricultural and Food Chemistry, 64(37):7014-7021.
    13.
  13. Edrispour Z., Homaei A. 2022. Exploring in vitro effect of silver nanoparticles and Holothuria parva extracts on kinetics and stability of α‐ Biotechnology and Applied Biochemistry, 70(2): 885-894.
    14.
  14. Eisapour M., Salari Aliabadi M.A., Salamat N., Nafisi Bahabadi M., Salati A.P. 2022. Identification and taxonomy of sea cucumbers (Holothuria) in Persian Gulf. Iranian Journal of Fisheries Sciences, 21(1):63-81.
    15.
  15. El Barky A.R., Ali E.M.M., Mohamed T.M. 2017. Marine sea cucumber saponins and diabetes. Austin Pancreat Disorders, 1(1):1-7.
    16.
  16. Fatemi S.M.R., Ghavam Mostafavi P., Hamiz Z. 2011. Identifcation of sea cucumbers of genus Holothuridae from intertidal zone of Gheshm, Persian Gulf, Iran. Iran Journal Oceanography, 7(2):57-65. (In Persian).
    17.
  17. Goldenberg R., Punthakee Z. 2013. Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome. Canadian Journal of Diabetes, 37:8-11.
    18.
  18. Gulçin İ., Taslimi P., Aygün A., Sadeghian N., Bastem E., Kufrevioglu O.I., Turkan F., Şen F. 2018. Antidiabetic and antiparasitic potentials: Inhibition effects of some natural antioxidant compounds on α-glycosidase, α-amylase and human glutathione S-transferase enzymes. International Journal of Biological Macromolecules, 119:741-746.
    19.
  19. Gupta R., Gigras P., Mohapatra H., Goswami V.K., Chauhan B. 2003. Microbial α-amylases: a biotechnological perspective. Process Biochemistry, 38(11):1599-1616.
    20.
  20. Harnedy P.A., FitzGerald R.J. 2012. Bioactive peptides from marine processing waste and shellfish: A review. Journal of Functional Foods, 4(1):6-24.
    21.
  21. Jimeno J., Faircloth G., Sousa-Faro J.F., Scheuer P., Rinehart K. 2004. New marine derived anticancer therapeutics- a journey from the sea to clinical trials. Marine Drugs, 2(1):14-29.
    22.
  22. Jo S.H., Ka E.H., Lee H.S. 2009. Comparison of antioxidant potential and rat intestinal α-glucosidases inhibitory activities of quercetin, rutin, and isoquercetin. International Journal of Applied Research in Natural Products, 2:52-60.
    23.
  23. Kariya Y., Watabe S., Hashimoto K., Yoshida K. 1990. Occurrence of chondroitin sulfate E in glycosaminoglycan isolated from the body wall of sea cucumber Stichopus japonicus. Journal of Biological Chemistry, 265(9):5081-5085.
    24.
  24. Kaur N., Kumar V., Nayak S.K., Wadhwa P., Kaur P., Sahu S.K. 2021. Alpha‐amylase as molecular target for treatment of diabetes mellitus: A comprehensive review. Chemical Biology & Drug Design, 98(4):539-560.
    25.
  25. Kermasha S. 2011. Antioxidants in digestive tracts and gonads of green urchin (Strongylocentrotus droebachiensis). Journal of Food Composition and Analysis, 24(2):179-183.
    26.
  26. Krish S., Das A. 2014. In-vitro bioactivity of marine seaweed, Cladophora rupestris. International Journal of Pharmacy and Biological Sciences, 5:898-908.
    27.
  27. Kumar S., Khare S.K. 2015. Chloride activated halophilic α-amylase from Marinobacter EMB8: Production optimization and nanoimmobilization for efficient starch hydrolysis. Enzyme Research, 2015:1-9.
    28.
  28. Li W., Dai R.J., Yu Y.H., Li L., Wu C.M., Luan W.W., Meng W.W., Zhang X.S., Deng Y.L. 2007. Antihyperglycemic effect of Cephalotaxus sinensis leaves and GLUT-4 translocation facilitating activity of its flavonoid constituents. Biological and Pharmaceutical Bulletin, 30(6):1123-1129.
    29.
  29. Mac Tavish T., Stenton-Dozey J., Vopel K., Savage C. 2012. Deposit-feeding Sea cucumbers enhance mineralization and nutrient cycling in organically-enriched coastal sediments. PloS one, 7(11): e50031.
    30.
  30. Mamlona J., Pelletier É., Girard-Lalancette K., Legault J., Karboune S. Kermasha S. 2011. Antioxidants in digestive tracts and gonads of green urchin (Strongylocentrotus droebachiensis). Journal of Food Composition and Analysis, 24(2):179-183.
    31.
  31. Marmouzi I., Tamsouri N., El Hamdani M., Attar A., Kharbach M., Alami R., EI Jemli M., Cherrah Y., Ebada S.S., Faouzi M.E.A. 2018. Pharmacological and chemical properties of some marine echinoderms. Revista Brasileira de Farmacognosia, 28:575-581.
    32.
  32. Meydi, Suwandi R., Suptijah P. 2016. Isolation of Compounds of Steroids Teripang Gamat (Stichopus variegatus) with Various Types of Solvents. Jurnal Pengolahan Hasil Perikanan Indonesia, 19(3):362-369.  
    33.
  33. Miller G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3):426-428.
    34.
  34. Mohebbi G.H., Nabipour I., Vazirizadeh A. 2014. The Sea, the future pharmacy. Iranian South Medical Journal, 17(4):748-788. (In Persian)
    35.
  35. Mojica E.R.E., Merca F. E. 2005. Biological properties of lectin from sea cucumber (Holothuria scabra Jäger). Journal of Biological Sciences, 5(4):472-477.
    36.
  36. Nagy M.A., Ewais M.M. 2014. Antidiabetic and antioxidative potential of Cystoseira myrica. Amrican Journal Biochemistery, 4:59-67.
    37.
  37. Nguyen T.H., Wang S.L., Nguyen A.D., Doan M.D., Tran T. N., Doan C.T., Nguyen V.B. 2022. Novel α-amylase inhibitor hemi-pyocyanin produced by microbial conversion of chitinous discards. Marine Drugs, 20(5): 283.  
    38.
  38. Nwosu F., Morris J., Lund V.A., Stewart D., Ross H.A. McDougall G.J. 2011. Anti-proliferative and potential anti-diabetic effects of phenolic-rich extracts from edible marine algae. Food Chemistry, 126(3):1006-1012.
    39.
  39. Ogunyemi O.M., Gyebi G.A., Ibrahim I.M., Esan A.M., Olaiya C.O., Soliman M.M., Batiha G.E.S. 2022. Identification of promising multi-targeting inhibitors of obesity from Vernonia amygdalina through computational analysis. Molecular Diversity, 27(1):1-25.
    40.
  40. Ojewole J.A. 2002. Hypoglycaemic effect of Clausena anisata (Willd) Hook methanolic root extract in rats. Journal of Ethnopharmacology, 81(2):231-237.
    41.
  41. Padauleng N., Nurhidayati N. 2016. Holothuria scabra memperbaiki fibrosis hepar pada tikus yang diinduksi karbon tetraklorida. Jurnal Kedokteran Brawijaya, 29: 139-142.
    42.
  42. Pandey S., Sree A., Dash S.S., Sethi D.P., Chowdhury L. 2013. Diversity of marine bacteria producing beta-glucosidase inhibitors. Microbial Cell Factories, 12:1-7.
    43.
  43. Paulay G. 2014. Holothuroidea. World Register of Marine Species. Retrieved on, 2
    44.
  44. Pawson D.L. 2007. Phylum echinodermata. Zootaxa, 1668: 749-764.
    45.
  45. Peyrot des Gachons C., Breslin P.A. 2016. Salivary amylase: digestion and metabolic syndrome. Current Diabetes Reports, 16:1-7.
    46.
  46. Ponnusamy S., Ravindran R., Zinjarde S., Bhargava S., Ravi Kumar, A. 2010. Evaluation of traditional Indian antidiabetic medicinal plants for human pancreatic amylase inhibitory effect in Vitro. Evidence-Based Complementary and Alternative Medicine, 2011:1-10.
    47.
  47. Prabhu A.S., Ananthan G. 2014. Alpha-amylase inhibitory activities of ascidians in the reatment of diabetes mellitus. Bangladesh Pharmacological Society, 9(4):498-500.
    48.
  48. Rasyid A., Yasman Y., Putra M.Y. 2021. Current prospects of nutraceutical and pharmaceutical use of sea cucumbers. Pharmacia, 68(3):561-572.
    49.
  49. Ravi C., Karthiga A. Venkatesan V. 2012. Isolation and biomedical screening of the tissue extracts of two marine gastropods Hemifusus pugilinus (Born, 1778) and Natica didyma (Roding, 1798). Asian Fisheries Science, 25:158-169.
    50.
  50. Rodeiro I., Olguín S., Santes R., Herrera J.A., Pérez C.L., Mangas R., Hernández Y., Fernández G., Hernández I., Hernández-Ojeda S., Camacho-Carranza R., Valencia-Olvera A., Espinosa-Aguirre J.J. Gas chromatography-mass spectrometry analysis of Ulva fasciata (green seaweed) extract and evaluation of its cytoprotective and antigenotoxic effects. Evidence - Based Complementary and Alternative Medicine, 2015:1-11.
    51.
  51. Samrot A., Vijay A. 2008. a-Amylase activity of wild and mutant strains of Bacillus The Internet Journal of Microbiology, 6(2):1-4.
    52.
  52. Sigwart J.D., Blasiak R., Jaspars M., Jouffray J.B., Tasdemir D. 2021. Natural Product Reports, 38:1235-1242.
    53.
  53. Silchenko A.S., Kalinovsky A.I., Avilov S.A., Andryjaschenko P.V., Dmitrenok P.S., Kalinin V.I., Chingizova E.A., Minin K.V., Stonik, V.A. 2016. Structures and biogenesis of fallaxosides D4, D5, D6 and D7, trisulfated non-holostane triterpene glycosides from the sea cucumber Cucumaria fallax. Molecules, 21:939.
    54.
  54. Soleimani S., Moein S., Yousefzadi M., Amrollahi Bioki N. 2017. Determination of in vitro antioxidant properties, anti-inflammatory effects and A-amylase inhibition of purple sea urchin extract of Echinometra mathaei from the Persian Gulf. Jundishapur Journal of Natural Pharmaceutical Products, 12:
    55.
  55. Stonik V.A. 1986. Some terpenoid and steroid derivatives from echinoderms and sponges. Pure and Applied Chemistry, 58:423-436.
    56.
  56. Sun Z., Chen F. 2012. Evaluation of the Green Alga Chlorella pyrenoidosa for management of diabetes. Journal of Food and Drug Analysis, 20(1):246-249.
    57.
  57. Tajik A., Keshavarz M., Homaei A. 2022. Inhibitory effect of Phallusia nigra (Savigny, 1816) extract on activity of Alpha –Amylase. Journal of Animal Biology, 14:1-15 (In Persian)
    58.
  58. Taouis M., Dagou C., Ster C., Durand G., Pinault M., Delarue J. 2002. N-3 polyunsaturated fatty acids prevent the defect of insulin receptor signaling in muscle. American Journalof Physiology: Endocrinology and Metabolism, 282:664-671.
    59.
  59. Tiwari P., Rahuja N., Kumar R., Lakshmi V., Srivastava M.N., Agarwal S.C., Raghubir R., Srivastava A.K. 2008. Search for antihyperglycemic activity in few marine flora and fauna. Indian Journal of Science and Technology, 1:1-5.
    60.
  60. Uthicke S., Schaffelke B., Byrne M. A boom-bust phylum? Ecological and evolutionary consequences of density variations in echinoderms. Ecological Monographs, 79(1):3-24.
    61.
  61. Vinoth Kumar R., Murugesan S., Shettu N. 2017. Anti-diabetic potential of marine red alga Champia parvula ( agardh) by inhibiting key metabolic enzymes. World Journal of Pharmaceutical Research, 6(10):1466-1474.
    62.
  62. Wei C.Y., Liao N.B., Zhang Y., Ye X.Q., Li S., Hu Y.Q., Liu D.H., Linhardt R.J., Wang X., Chen S.G. 2017. In vitro fermentation behaviors of fucosylated chondroitin sulfate from Pearsonothuria graeffei by human gut microflora. International Journal of Biological Macromolecules, 102:1195-1201.
    63.
  63. Yamazaki H., Sumilat D.A., Kanno S., Ukai K., Rotinsulu H., Wewengkang D.S., Ishikawa M., Mangindaan R.E., Namikoshi M. 2013. A polybromodiphenyl ether from an Indonesian marine sponge Lamellodysidea herbacea and its chemical derivatives inhibit protein tyrosine phosphatase 1B, an important target for diabetes treatment. Journal of Natural Medicines, 67:730-735.
    64.
  64. Zarei M.A., Poursharifi M. 2015. Searching for alpha-glucosidase inhibitory activity in hexane extracts by some plants from Kurdistan province. International Journal of Advanced Biological and Biomedical Research, 3(3):291-296.
    65.
  65. Zhu C.F., Li G.Z., Peng H.B., Zhang F., Chen Y., Li Y. 2010. Effect of marine collagen peptides on markers of metabolic nuclear receptors in type 2 diabetic patients with/without hypertension. Biomedical and Environmental Sciences, 23(2):113-120.
    66.
  66. Zinjarde S.S., Bhargava S.Y., Kumar A. R. 2011. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complementary and Alternative Medicine, 11:1-10.
    67.
_||_

Sanad

Sanad is a platform for managing Azad University publications

Links

Related Centers

Technical Support

Official pages

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]