The Effects of Various Concentrations of IBA Hormone on Rooting of ‘TF92’; New Peach-Almond Hybrid under Ex- vivo and In - vitro Conditions

محورهای موضوعی : Almond

Ruhollah Haghi ¹ , Ali Imani ² , Ali Torkashvand ³ , Marzieh Ghanbari Jahromi ⁴

1 - Department of Horticulture, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Temperate Fruits Research Center, Institute of Horticultural Sciences, Agricultural Research, Education and Extension Organization, Karaj, Iran
3 - Department of Horticulture, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Horticulture, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

تاریخ دریافت : 1402/01/31 تاریخ پذیرش : 1402/06/26 تاریخ انتشار : 1403/03/19

کلید واژه: Auxin, Explant, Hardwood cuttings, ‘TF92’ rootstock,

چکیده مقاله :

One of the main problems of fruit cultivation, especially peaches and almonds, is the lack of the application of the existing clone rootstocks such as the promising hybrid of peaches and almonds and its unavailability due to its propagation in Iran. So the seedling rootstocks are inevitably used. However these rootstocks cause many problems for the growers due to the lack of uniformity. In order to solve this problem, the effect of different concentrations of indole butyric acid on the rooting of hardwood cuttings ‘TF92’ (promising hybrid of peach and almond) was tested in a private sector greenhouse in Karaj equipped with a heating pad during 2019. In this paper, the effect of 4 different levels of indole butyric acid hormone (IBA) 0, 1500, 2500 and 3500 parts per million in solution on hardwood cuttings was studied in a completely randomized design in three replications. The results showed that there was a significant difference between various concentrations of indole butyric acid in terms of the effect on the characteristics of the mode of investigation such as the percentage of rooting, the number of roots formed, the dry weight of the roots, the length of the roots and the length of the resulting branches in the cuttings. Thus the highest rooting percentage (78.45) was found in the indole butyric acid (IBA) hormone treatment with a concentration of 2500 mg L-1 compared to other treatments. Therefore, this method can be recommended as a suitable treatment for the rooting of wood cuttings of this promising emerging rootstock. Also, the highest percentage of rooted plantlets was obtained on Murashige and Skoog medium (MS) supplemented with 1 mg L-1 IBA. According to this study, the concentrations of 1 mg L-1 IBA results in the maximum rooting, and it has been suggested as a suitable hormone for in vitro rooting of ‘TF92’.

چکیده انگلیسی:

One of the main problems of fruit cultivation, especially peaches and almonds, is the lack of the application of the existing clone rootstocks such as the promising hybrid of peaches and almonds and its unavailability due to its propagation in Iran. So the seedling rootstocks are inevitably used. However these rootstocks cause many problems for the growers due to the lack of uniformity. In order to solve this problem, the effect of different concentrations of indole butyric acid on the rooting of hardwood cuttings ‘TF92’ (promising hybrid of peach and almond) was tested in a private sector greenhouse in Karaj equipped with a heating pad during 2019. In this paper, the effect of 4 different levels of indole butyric acid hormone (IBA) 0, 1500, 2500 and 3500 parts per million in solution on hardwood cuttings was studied in a completely randomized design in three replications. The results showed that there was a significant difference between various concentrations of indole butyric acid in terms of the effect on the characteristics of the mode of investigation such as the percentage of rooting, the number of roots formed, the dry weight of the roots, the length of the roots and the length of the resulting branches in the cuttings. Thus the highest rooting percentage (78.45) was found in the indole butyric acid (IBA) hormone treatment with a concentration of 2500 mg L-1 compared to other treatments. Therefore, this method can be recommended as a suitable treatment for the rooting of wood cuttings of this promising emerging rootstock. Also, the highest percentage of rooted plantlets was obtained on Murashige and Skoog medium (MS) supplemented with 1 mg L-1 IBA. According to this study, the concentrations of 1 mg L-1 IBA results in the maximum rooting, and it has been suggested as a suitable hormone for in vitro rooting of ‘TF92’.

منابع و مأخذ:

Ak BE, Karahan Kiyar l, Hatipoglu P, Dikmetas HB (2021) Effects of different BA and IBA concentrations on proliferation and rooting of ‘GARNEM’ rootstock in vitro propagation. International Journal of Agriculture, Environment and Food Sciences. 5(4), 470-476. Doi: https://doi.org/10.31015/jaefs.2021.4.6

Antonopoulou C, Dimassi K, Therios I, Chatzissavvidis C, Tsirakoglou V (2005) Inhibitory effects of riboflavin (Vitamin B2) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (Prunus amygdalus × P. persica). Scientia Horticulturae. 106, 268–272.

Bertoni G (2011) Indolebutyric Acid–Derived Auxin and Plant Development. Plant Cell. 23(3):845. doi: 10.1105/tpc.111.230312. PMCID: PMC3082264.

Bielsa B, Sanz M, Rubio-Cabetas M (2019) Uncovering early response to drought by proteomic, physiological and biochemical changes in the almond × peach rootstock ‘Garnem’. Functional Plant Biology. 46, 994-1008.

Coli ́c S, Zec G, Baki ́c I, Jankovi ́c Z, Rahovi ́c D, Fotiri ́c A, kši ́c M(2018) Rootstock effect on some quality characteristics of almond cultivars ‘Troito’, ‘Marcona’ and ‘Texas’. Acta Horticulturea. 1219, 19–24.

Day LH (1953) Rootstock for stone fruits: Observations and Experiment with Plum, Peach, Apricot and Almond Roots for Stone Fruits. California Agricultural Experiment Station Bulletin. pp.736.

Duncan R, Connell J, Jarvis-Shean K, Brar G, Yaghmour M (2019) Field Evaluation of Almond Rootstock. In Annual Research Report 2019–2020; Almond Board of California: Sacramento, CA, USA. pp. 24.

Ersoy N, Aydin M (2008) The effect of some hormone and humidity levels on rooting of Mahaleb (Prunus Mahaleb) soft wood top cutting. Suleyman Demirel Universitiesi Ziraat Fakultesi Degisi. 3(1), 32-41.

Felipe AJ, Gómez-Aparisi J, Socías R, Carrera, M, (1997) The almond × peach hybrid rootstocks breeding program at Zaragoza (Spain). Acta Horticulturea. 451, 259-262. DOI:10.17660/ActaHortic.1997.451.28

Felipe AJ, Socias i Company R, Gomez J (1984) The Almond rootstock ideotype: II international symposium on pistachios and almond. Acta Hortculrea. 470,187-187.

Gudarowska E, Malaczukm L (2006) The quality of root system of dwarf rootstock, Pumi select, for Peach tree. Latvin Journal of Agronomy No.9.LLU.24-27

Guerrero JR, Garrido G, Acosta M, Sanchez-Bravo J (1999) Influence of 2, 3, 5-triiodobenzoic acid and 1-N-naphthylphthalamic acid on indole acetic acid transport in carnation cuttings: relationship with rooting. Journal of Plant Growth Regulation. 18, 183–190.

Hartmann HT, Kester DE (1983) Plant propagation, principles and practices Fourth Ed. Prentice-Hill, INC Engleood Cliffs, New Jersy, USA.

Howard RS, Harrison M, Fenlon CA (1983) Effective auxin treatment of leafless winter cutting. pp. 73-85 in: Monograph 10, Growth regulators in root development. Jackson M B; Stead A D ed. British Plant Growth Regulator Group.

Johnson E, Preece J, Aradhya M, Gradziel T (2020) Rooting response of Prunus wild relative semi-hardwood cuttings to indole-3-butyric acid potassium salt (KIBA). Scientia Horticulturea. 263, 109 - 144.

Kasim NE, Abou Rayya MS, Shaheen MA, Yehia TA, Ali EL (2009) Effect of different collection times and some treatments on rooting and chemical internal constituents of bitter almond hardwood cuttings. Research Journal of Agriculture and Biological Sciences. 5(2), 116-122.

Kaur S (2017). Evaluation of different doses of indole-3-butyric acid (IBA) on the rooting, survival and vegetative growth performance of hardwood cuttings of Flordaguard peach (Prunus persica L. Batch). Journal of Applied and Natural Science. 9(1), 173 - 180 .

Kreen S, Svenssonb M, Rumpunen K (2002) Rooting of clematis microshoots and stem cuttings in different substrates. Scientia Horticulturae. 351-357. 4238(2), 126-127

Kester DE, Sartori E (1966) Rooting of cuttings in populations of peach (P. persica L.), almond (P. amygdalus Batsch) and their F1 hybrids. Proceeding of American Society of Horticultural Science. 88, 219-23.

Kester DE, Grasselly C (1987) Almond rootstocks. In Rootstocks for Fruit Crops; Rom, R.C., Carlson, R.F., Eds.; John Wiley: New York, NY, USA. pp. 265–293.

Kester DE, Gradzeil TM, Grasselly C (1991) Almond in Genetic resources of temperate fruit and crops. Acta Hortculrea. 290, 123-149.

Kodad S, Melhaoui R, Hano C, Addi M, Sahib N, Elamrani A, Abid M, Mihamou A (2021) Effect of Culture Media and Plant Growth Regulators on Shoot Proliferation and Rooting of Internode Explants from Moroccan Native Almond (Prunus dulcis Mill.) Genotypes. Hindawi International Journal of Agronomy. Article ID 9931574, 10 pages.

Kordzadeh S, Sarikhani H (2021) Effect of different concentrations of indole butyric acid, putrescine and hydrogen peroxide on stem cuttings of the rootstock GF677 (Prunus amygdalus × Prunus persica) according to the cutting season. Revista Facultad Nacional de Agronomía Medellin. 74, 9571–9582.

Loreti F, Morini S (2008) Propagation techniques. Pp.221-243. Edit Layne, R. and D., Bassi. The peachBotany, Production and Uses. CAB International. pp. 516

Magyar-Tábori K, Dobránszki J, Bulley SM, Teixeira da Silva JA, Hudák I (2010) The role of cytokinins in shoot organogenesis in apple. Plant Cell, Tissue and Organ Culture. 101, 251–267. 

McLeod A, Vining K, Hoskins T, Contreras R (2022) Impact of indole-3-butyric acid concentration and formulation and propagation environment on rooting success of ‘I3’ hemp by stem cuttings. HortTechnology. 32(3), 321-324.

Mirsolimani A, Rahmi M (2016) The effects of two types of artificial auxin on the rooting of peach x almond hybrid hardwood cuttings in open space conditions. Agriculture and horticulture. 76, 96-89.

Moreno MA, Cambra R (1994) Adarcias: an almond × peach hybrid rootstock. HortScience 29, 925-929.

Moreno MA, Tabuenca MC, Cambra R(1994)Performance of Adafuel and Adarcias as peach rootstocks. HortScience. 29, 1271–1273.

Pallardy SG (2008) Physiology of Woody Plants (Third Edition) 469Publisher: Elsevier, E-ISBN: 9780120887651

Pinto KGD, Albertino SMF, Leite BN, Pereira Soares DO, Castro FM (2023) d, Gama LA. d., Clivati, D., & Atroch, A. L. (2020). Indole-3-butyric Acid Improves Root System Quality in Guarana Cuttings, HortScience 55(10), 1670-1675. Retrieved Mar 9, 2023, from https://doi.org/10.21273/HORTSCI14984-20

Sarikhani H, Sarikhani-khorami H (2020) Effect of Light Quality on Micropropagation and Some Morphological Properties of Cadaman Avimag (Prunus persica × P. davidiana) Rootstock. International Journal of Horticultural Science and Technology. 8(1), 51-65

Shamshad M, Abbasi A(2003) Effect of IBA on hardwood cutting of peach rootstocks under greenhouse conditions. Asian Journal of Plant Sciences. 2(3), 265-269.

Socias i Company R, Go´mez Aparisi J, Felipe A (1995) A genetical approach to iron chlorosis in deciduous fruit trees. In: Abadia, J. (Ed.), iron nutrition in soil and plants. Kluwer Academic Publishers, Dordrecht, the Netherlands. pp. 167–174.

Socias i Company R, Gradziel TM (2017) Almonds: Botany, Production and Uses. Boston, MA: CABI.

Stefanic M, Vodnik D (2007) The effect of fogging system on the physiological status and rooting capacity of leafy of wood species. Tree – Structre and Function. 27, 441- 496.

Swedan AA, Edriss MH, Abd-Alhamed MF, Yusre A (1993) Root initiation in the plum rootstock Marianna and the promotive effects of co-factors. Egyptian Journal of Horticultural Science. 20(1), 43-55.

Tewfic AA(2002) Effect of IBA, planting media and type of cutting on rooting of new a guard peach rootstock under Egyptian conditions. Acta Horticulturea. Article number 592_24. 169-175.

Vahdati K, Sadeghi-Majd R, Sestras AF, Licea-Moreno RJ, Peixe A, Sestras RE (2022) Clonal propagation of walnuts (Juglans spp.): A review on evolution from traditional techniques to application of biotechnology. Plants. 11, 3040.

Vahdati K, Sarikhani S, Arab, MM, Leslie CA, Dandekar AM, Aletà N,  Bielsa B, Gradziel TM, Montesinos Á, Rubio-Cabetas MJ et al (2021) Advances in Rootstock Breeding of Nut Trees: Objectives and Strategies. Plants. 10, 2234.

Webster AD (1995) Temperate fruit tree rootstock propagation. New Zealand Journal of Crop and Horticultural Science. 23, 355-372.

Wojtania A, Markiewicz M, Waligórski P (2022) Regulation of the Bud Dormancy Development and Release in Micropropagated Rhubarb 'Malinowy'. International Journal of Molecular Sciences. 23(3), 1480. doi: 10.3390/ijms23031480. PMID: 35163404; PMCID: PMC8835828.

Yang Y, Liu X, Jiang Y, Xiang Z, Xu Q, Zhao N (2015) Root growth, free amino acids, and carbohydrates of tall fescue in response to soil salinity. HortScience. 50(4), 609-614.