Effect of Sulfur on Toxigenic Aspergillus flavus In Vitro

محورهای موضوعی : Plant Pathology

Arezou Kazemi ¹ (Plant Pathology M.Sc. graduated and Associate Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran)
Ebrahim Sedaghati ² (Plant Pathology M.Sc. graduated and Associate Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran)
Seyed Reza Fani ³ (Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran)
Mohammad Moradi ⁴ (Pistachio Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran)
Marieh Nadi ⁵ (Pistachio Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran)

تاریخ دریافت : 1401/03/17 تاریخ پذیرش : 1402/02/05 تاریخ انتشار : 1402/09/10

کلید واژه: sporulation, Mycotoxin, Food Safety, Fungal growth, Mycelium weight,

چکیده مقاله :

Application of sulfur to control the common pistachio psylla has been nowadays popular. The psylla is one of the main pistachios’ pests. Aspergillus flavus, the most important aflatoxin producer, has a strong impact in the mycoflora of pistachio orchards. The present study has been conducted to determine the effects of sulfur on A. flavus. Therefore, a toxigenic A. flavus strain was cultured on potato dextrose agar (PDA) medium, and the effects of mineral sulfur, refinery sulfur and sodium metabisulfite were closely monitored on fungal mycelial dry weight, germination of spores, germ tube and mycelium germination. The Fungal parameters measurement for sulfur vapor exposure and sulfur addition to the culture medium declared no significant effect on the growth of A. flavus mycelium in the direct addition. But sulfur in liquid medium as well as in high concentrations caused mycelial dry weight loss. Importantly, the sublimation of 0, 0.5, 1, 2, 5, 10, 25 and 50 gm-3 from the refinery sulfur, prevented completely the growth of germinated spores and mycelial. Significantly, the complete inhibition of spore germination was observed at a concentration level of 2 g m-3 and also sodium metabisulfite prevented the growth of A. flavus at a concentration of 15 g l-1 culture medium. The sulfur usage in culture medium declared no inhibition of A. flavus growth, but at high concentrations in liquid medium, it reduced mycelial dry weight. Therefore, it is found that the sulfur application in any kind to control of psylla would not probably effect significantly on the aflatoxigenic A. flavus.

منابع و مأخذ:

Aktas T (2015) Some applications to retain the quality of fruits and vegetables during drying and storage in Turkey.Japan Journal of Food Engineering. 16(1), 29-36. Doi: 10.11301/jsfe.16.29

FAO (2020) Pistachio production in 2018, Crops/Regions/World list/Production Quantity (pick lists). UN Food and Agriculture Organization, Corporate Statistical Database (FAOSTAT). Available: https: //www. fao.org/faostat/ en/#data/QCL. . 2019. [Accessed February 24 2020].

Gammon DW, Moore TB, O’Malley MA (2010) A toxicological assessment of sulfur as a pesticide. In Hayes' Handbook of Pesticide Toxicology. Academic Press. Doi: 10.1016/B978-0-12-374367-1.00088-4

Gunduz S, Akman S (2014) Sulfur determination in some nuts and dried fruits sold in Turkey by high resolution graphite furnace molecular absorption spectrometry. LWT-Food Science and Technology. 59(2), 718-723. Doi: 10.1016/j.lwt.2014.05.022

Hosseini N, Rezanejad F, ZamaniBahramabadi E (2022) Effects of soil texture, irrigation intervals, and cultivar on some nut qualities and different types of fruit blankness in pistachio (Pistacia vera L.). International Journal of Horticultural Science and Technology. 9(1), 41-53. Doi: 10.22059 /ijhst.2020.303725.374

Jiang C, Shi J, Chen X, Liu Y (2015) Effect of sulfur dioxide and ethanol concentration on fungal profile and ochratoxin a production by Aspergillus carbonarius during wine making. Food Control. 47, 656-663. Doi: 10.1016/j.foodcont.2014.08.011

Kim YH, Kim GH, Yoon KS, Shankar S, Rhim JW (2020) Comparative antibacterial and antifungal activities of sulfur nanoparticles capped with chitosan. Microbial Pathogenesis 144: 104178. Doi: 10.1016/j.micpath.2020.104178

Kolaei EA, Tweddell RJ, Avis TJ (2012) Antifungal activity of sulfur-containing salts against the development of carrot cavity spot and potato dry rot. Postharvest Biology and Technology. 63(1), 55-59. DOI: 10.1016/j.postharvbio.2011.09.006

Mahbobinejhad Z, Aminian H, Ebrahimi L, Vahdati K (2019) Reduction of aflatoxin production by exposing Aspergillus flavus to CO₂. Journal of Crop Protection. 8(4), 441-448.

Martínez-Blay V, Taberner V, Pérez-Gago MB, Palou L (2020) Control of major citrus postharvest diseases by sulfur- containing food additives. International Journal of Food Microbiology. 330: Doi: 108713. 10.1016/j.ijfoodmicro.2020.108713

Melanitou MA (1995) Sulphur dried figs in Greece: Technological aspects and aflatoxin contamination. University of Bath, UK.

Moradi M, Fani SR (2018) Review of aflatoxin in pistachio and its control strategies. Plant Pathology. 7(2), 22-33.  Doi: 10. 2982/ PPS.7.2.22

Moradi M, Hokmabadi H (2011) Control of mycotoxin bioactives in nuts: farm to fork. In Tokusoglu, Ö (ed) Nut and cereal bioactives sources. Chemistry, and applications. CRC Press. Turkey.

Nazoori F, ZamaniBahramabadi E, Mirdehghan H (2022) Effect of sulfur pesticide on the quality of fresh pistachios in cold storage. International Journal of Horticultural Science and Technology. 9(4), 453-462. Doi: 10.22059/ijhst.2021.329211.497

Nazoori F, ZamaniBahramabadi E, Mirdehghan H, Yousefi M (2022) Preharvest application of sulfur as pesticide on fresh hull and kernel of pistachio (Pistacia vera L.). International Journal of Horticultural Science and Technology. 9(1), 117-129. Doi: 10.22059/ijhst.2021.313206.408

Olkowski AA (1997) Neurotoxicity and secondary metabolic problems associated with low to moderate levels of exposure to excess dietary sulphur in ruminants: a review. Veterinary and Human Toxicology. 39(6), 355- 360.

Sharifkhah M, Bakhshi D, Pourghayoumi M, Abdi S, Hokmabadi H (2020) Effect of pollination time on yield and antioxidant properties of some pistachio cultivars. International Journal of Horticultural Science and Technology. 7(1), 51-58. Doi: 10.22059/ijhst.2020.285527.306.

Shamshiri MH, Hasani MR (2015) Synergistic accumulative effects between exogenous salicylic acid and arbuscular mycorrhizal fungus in pistachio (Pistacia Vera cv. Abareqi) seedlings under drought stress. International Journal of Horticultural Science and Technology. 2(2), 151-160. Doi: 10.22059/ijhst.2015.56432

Studdert VP, Labuc RH (1991) Thiamin deficiency in cats and dogs associated with feeding meat preserved with sulphur dioxide. Australian Veterinary Journal. 68(2), 54-57. Doi:10.1111/j.1751-0813. 1991. tb03129.x

Wang P, Chang PK, Kong Q, Shan S, Wei Q (2019) Comparison of aflatoxin production of Aspergillus flavus at different temperatures and media: Proteome analysis based on TMT. International Journal of Food Microbiology. 310, 108313.