Effects of Using a Multi-Strain Probiotic on Performance, Immune Responses and Cecal Microflora Composition in Broiler Chickens Reared Under Cyclic Heat Stress Condition
محورهای موضوعی : Camel
1 - Young Researchers and Elite Club, Khorasgan Branch, Islamic Azad University, Isfahan, Iran
2 - Young Researchers and Elite Club, Khorasgan Branch, Islamic Azad University, Isfahan, Iran
کلید واژه: Performance, Broiler, immunity, cecal microflora, cyclic heat stress, Probiotic,
چکیده مقاله :
This experiment was conducted to examine the effect of multi-strain probiotic (Primalac) on performance, immune responses and cecal microflora in broiler chickens under cyclic heat stress condition. A total of 96 one-d-old mixed sex broiler chicks (Ross 308) were weighed and randomly allocated to two treatment groups, each with 4 replicate pens of 12 chicks. The dietary treatments were basal diet (control) andcontrol +0.9 g Primalac/kg diet. Body weights of broilers were determined at d 1, 21 and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. The populations of Lactobacilli spp and coliforms were enumerated in the cecum. Antibody titers against Newcastle, Bronchitis, and Gumboro were measured as immune responses at 28 d of age. As a result of this study, use of probiotic significantly(P<0.05) increased broiler performance by enhancing body weight, daily feed intake and decreasing the feed conversion ratio.The Lactobacilli spp. population in birds supplemented with probiotic significantly was higher and coliforms population was lower than control groups at 42 d of age (P<0.05).Also administration of the probiotic appeared to improve the antibody responses to Newcastle diseasevirus and Bronchitis, Gumboro disease vaccination. In conclusion, the results indicate that supplementing broiler reared under heat stress condition with 0.9 g Primalac/kg diet could induce favorable influences on performance, immune responses and cecal microflora.
این پژوهش جهت بررسی اثرات استفاده از پروبیوتیک چند سویه‌ای (پریمالاک) در عملکرد، پاسخ‌های ایمنی و جمعیت میکروبی سکوم در جوجه‌های گوشتی پرورش یافته در استرس گرمایی انجام شد. در این آزمایش 96 قطعه جوجه گوشتی یکروزه (سویه راس 308) به‌طور تصادفی به 8 گروه 12 قطعه‌ای تقسیم و سپس با وجود 2 تیمار آزمایشی هر 4 گروه به‌طور تصادفی به یکی از تیمارهای آزمایشی اختصاص داده شدند. در طول دوره آزمایش (42-0 روزگی)، جوجه‌های گوشتی جیره پایه (کنترل) و جیره پایه مکمل شده با 9/0 گرم پریمالاک در کیلوگرم خوراک را دریافت کردند. میانگین مصرف خوراک روزانه، ضریب تبدیل غذایی و افزایش وزن روزانه در سنین 14، 28 و 42 روزگی تعیین شدند. جمعیت میکروبی لاکتوباسیلوس‌ها و کلیفرمها در سکوم اندازه‌گیری و محاسبه شدند. در سن 28 روزگی نمونه خون از ورید بالی برای اندازه‌گیری عیار پادتن بر علیه نیوکاسل، برونشیت، و گامبرو گرفته شد. نتایج حاصل از این تحقیق نشان داد که استفاده از پروبیوتیک به‌طور معنی‌داری (05/0P<) باعث افزایش عملکرد در جوجه‌های گوشتی گردید، به‌طوریکه وزن‌بدن و متوسط افزایش وزن روزانه افزایش و ضریب تبدیل خوراکی کاهش یافت. جمعیت میکروبی لاکتوباسیلوس‌ها در جوجه‌های مکمل شده با پروبیوتیک به‌طور معنی‌داری افزایش یافت و جمعیت کلیفرمها به‌طور معنی‌داری کاهش یافت (05/0P<). همچنین استفاده از پروبیوتیک عیار پادتن تولیدی بر علیه ویروس نیوکاسل، برونشیت و گامبرو را افزایش داد. از جمع‌بندی نتایج این تحقیق چنین به‌نظر می‌رسد که مکمل کردن جیره‌های جوجه‌های گوشتی پرورش یافته در شرایط استرس گرمایی با 9/0 گرم پریمالاک در کیلوگرم خوراک اثرات مطلوبی را در عملکرد، پاسخ‌های ایمنی و جمعیت میکروبی سکوم القا نمود.
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