Effects of Using a Multi-Strain Probiotic on Performance, Immune Responses and Cecal Microflora Composition in Broiler Chickens Reared Under Cyclic Heat Stress Condition
الموضوعات :
1 - Young Researchers and Elite Club, Khorasgan Branch, Islamic Azad University, Isfahan, Iran
2 - Young Researchers and Elite Club, Khorasgan Branch, Islamic Azad University, Isfahan, Iran
الکلمات المفتاحية: Performance, Broiler, immunity, cecal microflora, cyclic heat stress, Probiotic,
ملخص المقالة :
This experiment was conducted to examine the effect of multi-strain probiotic (Primalac) on performance, immune responses and cecal microflora in broiler chickens under cyclic heat stress condition. A total of 96 one-d-old mixed sex broiler chicks (Ross 308) were weighed and randomly allocated to two treatment groups, each with 4 replicate pens of 12 chicks. The dietary treatments were basal diet (control) andcontrol +0.9 g Primalac/kg diet. Body weights of broilers were determined at d 1, 21 and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. The populations of Lactobacilli spp and coliforms were enumerated in the cecum. Antibody titers against Newcastle, Bronchitis, and Gumboro were measured as immune responses at 28 d of age. As a result of this study, use of probiotic significantly(P<0.05) increased broiler performance by enhancing body weight, daily feed intake and decreasing the feed conversion ratio.The Lactobacilli spp. population in birds supplemented with probiotic significantly was higher and coliforms population was lower than control groups at 42 d of age (P<0.05).Also administration of the probiotic appeared to improve the antibody responses to Newcastle diseasevirus and Bronchitis, Gumboro disease vaccination. In conclusion, the results indicate that supplementing broiler reared under heat stress condition with 0.9 g Primalac/kg diet could induce favorable influences on performance, immune responses and cecal microflora.
Abdel Fattah S.A., El Sanhoury M.H., El Mednay N.M. and Abdel Azeem F. (2008). Thyroid activity, some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. Int. J. Poult. Sci. 7, 215-222.
Allan W.H. and Gough R.E. (1974). A standard haemagglutination inhibition test for Newcastle disease 1. Comparison of macro and micro method. Vet. Rec. 95, 120-123.
Apata D.F. (2008). Growth performance, nutrient digestibility and immune response of broiler chicks fed diets supplemented with a culture of Lactobacillus bulgaricus. J. Sci. Food Agric. 88, 1253-1258.
Ashayerizadeh A., Dabiri N., Mirzadeh K.H. and Ghorbani M.R. (2011). Effects of dietary inclusion of several biological feed additives on growth response of broiler chickens. J. Cell. Anim Biol. 5, 61-65.
Ayasan T., Ozcan B.D., Baylan M. and Canogullari S. (2006). The effects of dietary inclusion of probiotic protexin on egg yield parameters of Japanese quails (Coturnix coturnix Japonica). Int. J. Poult. Sci. 5, 776-779.
Bal A.P., Ouyang Q., Zhang W., Wang C.H. and Li S.F. (2004). Probiotics inhibit TNF-a-induced interleukin-8-secreation of HT29 cells. World J. Gastroenterol. 10, 455-457.
Cebra J.J. (1999). Influences of microbiota on intestinal immune system development. Am. J. Clin. Nutr. 69, 1051-1064.
Chichlowski M., Croom J., McBride B.W., Daniel L., Davis G. and Koci M.D. (2007). Direct fed microbial Primalac and Salinomycin modulate whole-body and intestinal oxygen consumption and intestinal mucosal cytokine production in the broiler chick. Poult. Sci. 86, 1100-1106.
Christensen H.R., Frokiaer H. and Pestka J.J. (2002). Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. J. Immunol. 168, 171-178.
Dallout R.A., Lillehoj H.S., Shellem T.A. and Doerr J.A. (2003). Enhanced mucosal immunity against Eimeria acervulina in broilers fed a Lactobacillus based probiotic. Poult. Sci. 82, 62-66.
Falaki M.,Shams Shargh M., Dastar B. and Zerehdaran S. (2011). Effects of different levels of probiotic and prebiotic on performance and carcass characteristics. J. Anim. Vet. Adv. 10, 378-384.
Farnell M.B., Donoghue A.M., De Los Santos F.S., Blore P.J., Hargis B.M., Tellez G. and Donoghue D.J. (2006). Upregulation of oxidative burst and degranulation in chicken heterophils stimulated with probiotic bacteria. Poult. Sci. 85, 1900-1906.
Gunal M., Yayli G., Kaya O., Karahan N. and Sulak O. (2006). The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int. J. Poult. Sci. 5, 149-155.
Haghighi H.R., Gong J., Gyles C.L., Hayes M.A., Zhou H., Sanei B., Parvizi P., Gisavi H., Chambers J.R. and Sharif S.H. (2005). Modulation of antibody mediated immune response by probiotics. Clin. Diagn. Lab. Immunol. 12, 1387-1392.
Haung M.K., Choi Y.J., Houde R., Lee J.W. and Zhao X. (2004). Effects ofLactobacilli and an acidophilus fungus on the production performance and immune responses in broiler chickens. Poult. Sci. 83, 788-795.
Higgins S.E., Higgins J.P., Wolfenden A.D., Henderson S.N., Torres Rodriguez A., Tellez G. and Hargis B. (2008). Evaluation of a Lactobacillus based probiotic culture for the reduction of Salmonella enteritidis in neonatal broiler chicks. Poult. Sci. 87, 27-31.
Howlinder M.A.R. and Rose S.P. (1989). Rearing temperature and meat yield of broilers. Br. Poult. Sci. 30, 61-67.
Lan P.T., Sakamoto M. and Benno Y. (2004). Effects of two probiotic Lactobacillus strains on jejunal and cecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16S rRNA genes. Microbiol. Immunol. 48, 917-929.
Leblanc J., Fliss I. and Matar C. (2004). Induction of a humoral immune response following an Escherichia coli O157:H7 infection with an immunomodulatory peptidic fraction derived from Lactobacillus helveticus-fermented milk. Clin. Diagn. Lab. Immunol. 11, 1171-1181.
LeesonS. (1986). Nutritional considerations of poultry during heat stress. World’ s Poult. Sci. J. 42, 69-81.
Li L.L., Hou Z.P., Li T.J., Wu G.Y., Huang R.L., Tang Z.R., Yang C.B., Gong J., Yu H. and Kong X.F. (2008). Effects of dietary probiotic supplementation on ileal digestibility of nutrients and growth performance in 1 to 42-day-old broilers. J. Sci. Food Agric. 88, 35-42.
Maassen C.B., Van Holten Neelen C., Balk F., Den Bak Glashouwer M.J., Leer R.J., Laman J.D., Boersma W.J. and Claassen E. (2000). Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains. Vaccine. 18, 2613-2623.
Mountzouris K.C., Tsirtsikos P., Kalamara E., Nitsch S., Schatzmayr G. and Fegeros K. (2007). Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcusand Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult. Sci. 86, 309-317.
Mountzouris K.C., Balaskas C., Xanthakos I., Tzivinikou A. and Fegeros K. (2009). Effects of a multi-species probiotic on biomarkers of competitive exclusion efficacy in broilers challenged with Salmonella enteritidis. Br. Poult. Sci. 50, 467-478.
Mountzouris K.C., Tsitrsikos P., Palamidi I., Arvaniti A., Mohnl M., Schatzmayr G. and Fegeros K. (2010). Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins and cecal microflora composition. Poult. Sci. 89, 58-67.
Nayebpor M., Farhomand P. and Hashemi A. (2007).Effects of different levels of direct fed microbial (Primalac) on growth performance and humoral immune response in broiler chickens. J. Anim. Vet. Adv. 6, 1308-1013.
Netherwood T., Gilbert H.J., Parker D.S. and O’Donnell A.G. (1999). Probiotics shown to change bacterial community structure in the avian gastrointestinal tract. Appl. Environ. Microbiol. 65, 5138-5143.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Academy Press, Washington, DC.
Panda A.K., Ramarao S.V., Raju M.V.L.N. and Sharma S.R. (2006). Dietary supplementation of probiotic Lactobacillus sporogenes on performance and serum biochemico-lipid profile of broiler chickens. J. Poult. Sci. 43, 235-240.
Racedo S., Villena J., Medina M., Aguero G., Rodrıguez V. and Alvarez S. (2006). Lactobacillus casei administration reduces lung injuries in a Streptococcus pneumoniae infection in mice. Microb. Infect. 8, 2359-2366.
Rahimi S.H. and Khaksefidi A. (2006). A comparison between the effects of a probiotic (Bioplus 2B) and an antibiotic (Virgenia virgeniamycin) on the performance of broiler chicken under heat stress. Iranian J. Vet. Res. 7, 48-56.
Rehman H., Vahjen W., Awad W.A. and Zentek J. (2007). Indigenous bacteria and bacterial metabolic products in the gastrointestinal tract of broilers.Arch. Anim. Nutr. 61, 319-335.
SAS Institute. (1996). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC.
Smirnov A., Perez E., Amit Romach E., Sklan D. and Uni Z. (2005). Mucin dynamics and microbial populations in chicken small intestine are changed by dietary probiotic and antibiotic growth promoter supplementation. J. Nutr. 135, 187-192.
Talebi A., Amirzadeh B., Mokhtari B. and Gahri H. (2008). Effects of a multi-strain probiotic (Primalac) on performance and antibody responses to Newcastle disease virus and infectious bursal disease virus vaccination in broiler chickens. Avian Pathol. 37, 509-512.
Teeter R.G. and Belay T. (1996). Broiler management during heat stress. Anim. Feed Sci. Technol. 58, 127-142.
Teo A.Y. and Tan H.M. (2007). Evaluation of the performance and intestinal gut microflora of broilers fed on corn-soy diets supplemented with Bacillus subtilis PB6 (CloSTAT). J. Appl. Poult. Res. 16, 296-303.
Vispo C. and Karasov W.H. (1997). Interaction of avian gut microbes and their host. Pp. 116-155 in Gastrointestinal Microbes and Host Interactions. R.J. Mackie, B.A. White and R.E. Issacson Eds. New York.
Yahav S., Straschnow A., Plavnik I. and Hurwitz S. (1996). Effect of diurnal cyclic versus constant temperatures on chicken growth and food intake. Br. Poult. Sci. 37, 43-54.
Yu B., Liu J.R., Hsiao F.S. and Chiou P.W.S. (2008). Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous β-glucanase as a probiotic in poultry diets based on barley. Anim. Feed Sci. Technol. 141, 82-91.
Zulkifli I., Abdullah N., Azrin N.M. and Ho Y.W. (2000). Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. Br. Poult. Sci. 41, 593-597.