Effects of Zinc Oxide Nanoparticles Supplementation on Mortality due to Ascites and Performance Growth in Broiler Chickens
محورهای موضوعی : Camel
1 - Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran
کلید واژه: growth performance, ascites, blood parameters, broilers, Zinc Oxide Nanoparticles,
چکیده مقاله :
Six hundred 1-d old male broilers (Ross 308) were assigned into four experimental groups; each was composed of 5replications of 30 birds including control (0), 10, 20 and 40 mg/kg nano-ZnO in the basal diets. Birds were exposed to low ambient temperature (15 to 18 ˚C) to induce ascites. Blood parameters including; activity of aspartate transferase (AST), alanine transferase (ALT), lactate dehydrogenase (LDH) and levels of protein, glucose, hemoglobin, hematocrit, white blood cell (WBC), red blood cell (RBC) and malondialdehyde (MDA) were determined. Mortalities were inspected to determine cause of death and diagnose of ascites. At the end of the experiment (day 42), 2 chickens from each replicate were randomly selected, slaughtered and ascites index calculated. Average body weight gain (ABWG) and average feed intake (AFI) were measured weekly and weekly average feed conversion ratio (AFCR) was calculated. Results showed that 40 mg/kg nano-ZnO significantly reduced MDA level in plasma and liver. Moreover, 20 and 40 mg/kg nano-ZnO had decreased significantly mortality due to ascites and ascites index. It is also, nano-ZnO in all levels had significantly increased ABWG. Birds in the 40 mg/kg nano-ZnO group had lower AFCR. It was concluded that nano-ZnO improved performance and reduced mortality due to ascites, and 40 mg/kg nano-ZnO is the optimal level in diets.
تعداد 600 قطعه جوجه گوشتی نر (راس 308) به چهار گروه آزمایشی ( 5 تکرار و 30 پرنده در هر تکرار) شامل 0، 10، 20 و 40 میلیگرم نانوذرات اکسید روی در کیلوگرم جیره پایه تقسیم شدند. برای القاء آسیت، همه پرندگان تحت برنامه دمایی سرد (15 تا 18 درجه سانتیگراد) قرار گرفتند. نمونه خونی شامل؛ فعالیت آنزیم آسپارتات آمینوترانسفراز، آلانین آمینوترانسفراز، لاکتات دهیدروژناز، پروتئین، هموگلوبین، هماتوکریت، گلبول قرمز، گلبول سفید و سطح مالون دیآلدئید اندازهگیری شدند. تلفات نیز جهت تعیین دلیل مرگ آسیتی، بررسی شد. روز آخر آزمایش (روز 42) از هر تکرار 2 قطعه پرنده به طور تصادفی انتخاب شده و بعد از کشتار، شاخص آسیتی محاسبه گردید. میانگین افزایش وزن بدن، میانگین خوراک مصرفی و ضریب تبدیل خوراک نیز ب طور هفتگی محاسبه گردید. نتایج نشان داد، سطح 40 میلیگرم نانو ذرات اکسید روی به طور معنیداری سبب کاهش سطح مالوندیآلدئید در پلاسما و کبد پرندگان شد. علاوه بر این، سطوح 20 و 40 میلیگرم سبب کاهش معنیدار شاخصی آسیتی و تلفات ناشی از آسیت شدند. همچنین، در حالیکه نانو ذرات اکسید روی در همه سطوح سبب افزایش وزن حاصله شد، سطح 40 میلیگرم سبب کاهش معنیدار ضریب تبدیل خوراک شد. نتیجه این تحقیق نشان داد که استفاده از نانو ذرات اکسید روی سبب بهبود عملکرد و کاهش تلفات آسیتی در جوجههای گوشتی شد و مناسبترین سطح آن در این 40 میلیگرم بود.
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