Liquid Metabolite of Lactobacillus plantarum and Putrescine Effects on Growth, Tissue Polyamine, Blood Lipids and Intestine Morphology of Broiler Chickens
محورهای موضوعی : Camelس.م. هاشمی 1 , تی.سی. لوه 2 , اچ.ال. فوو 3
1 - Department of Animal Science, Qom Agriculture and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Qom, Iran
2 - Department of Animal Science, University of Putra Malaysia, Malaysia
3 - Department of Bioprocess Technology, University of Putra Malaysia, Malaysia
کلید واژه: intestinal morphology, blood lipids, luminal polyamines, microbial metabolite, putre-scine,
چکیده مقاله :
This experiment aimed to investigate the effects of liquid metabolite (LM) produced by Lactobacillus plantarum and dietary putrescine (PUT) on growth, blood lipids, villus height (VH), crypt depth (CD) and polyamines (PAs) content of intestinal tissue and ileal digesta in chickens. Six treatments, replicated six time each, were factorial arrangements of two levels of LM (0 and 0.3%) and three levels of dietary putrescine (0, 0.03 and 0.05%). Growth performance and PAs content of digesta and excreta (at 21 d) were measured, as well as small intestine VH and CD. Blood cholesterol, triglyceride and glucose were measured at 24, 33 and 40th d. Putrescine (0.05%) negatively affected body weight, feed conversion ratio and protein and energy efficiency ratio while increased duodenal VH significantly (P<0.05) as compared to the 0.03% putrescine. Aging was effective on blood cholesterol, triglyceride and glucose. Blood triglyceride decreased by 0.03% putrescine (P<0.05). Faecal spermidine was increased significantly (P<0.05) by 0.05% putrescine. Duodenal PAs declined in the chickens fed LM, whereas dietary putrescine had no effects on intestinal tissue polyamine. In conclusion, luminal PAs content was not affected by treatments. LM influenced intestinal tissue PAs but had no effects on growth and ileal digesta polyamine content. However, putrescine (0.05%) was harmful to the growth but increased duodenal VH.
این آزمایش با هدف بررسی اثرات متابولیت مایع تولید‌شده توسط لاکتوباسیلوس پلانتاروم و پوترسین موجود در جیره غذایی بر عملکرد رشد، لیپیدهای خون، ارتفاع ویلی، عمق کریپت و پلی‌آمین‌های بافت روده و شیرابه گوارشی در جوجه‌های گوشتی انجام شد. شش تیمار غذایی با شش تکرار و با چینش فاکتوریل عبارت بودند از دو سطح متابولیت مایع (0 و 3/0 درصد) و سه سطح پوترسین (0، 03/0 و 05/0 درصد). عملکرد رشد و غلظت پلی‌آمین‌های شیرابه گوارشی روده و مدفوع به همراه ارتفاع ویلی و عمق کریپت در سن 21 روزگی اندازه‌گیری شد. کلسترول ، تری‌گلیسیرید و گلوکز خون در سنین 24، 33 و 40 روزگی اندازه‌گیری شدند. پوترسین (05/0 درصد) بر وزن بدن، ضریب تبدیل غذایی و راندمان مصرف انرژی و پروتئین تأثیر منفی داشت و در مقایسه با 03/0 درصد پوترسین باعث افزایش معنی‌دار ارتفاع ویلی در دئودنوم گردید (05/0P<). افزایش سن به طور معنی‌داری بر کلسترول، تری‌گلیسیرید و گلوکز خون اثر می‌گذارد. تری‌گلیسیرید خون تحت تأثیر 03/0 درصد پوترسین کاهش معنی‌دار یافت (05/0P<) و اسپرمیدین مدفوع به طور معنی‌داری در اثر پوترسین (05/0 درصد) افزایش یافت. پلی‌آمین‌های بافت دئودنوم روده در جوجه‌های تغذیه‌شده با متابولیت مایع کاهش یافتند در حالیکه میزان پوترسین جیره خوراکی تأثیری بر روی پلی‌آمین‌های بافت روده نداشت. به عنوان یک نتیجه کلی محتوای پلی‌آمین‌های شیرابه‌های گوارشی روده تحت تأثیر تیمار‌های آزمایش واقع نشدند. متابولیت مایع میکروبی با اینکه پلی‌آمین‌های بافت روده را تحت تأثیر قرار داد اما هیچگونه تأثیری بر رشد و غلظت پلی‌آمین‌های شیرابه روده نداشت. در همین حال پوترسین در سطح 05/0 درصد بر رشد جوجه‌ها تأثیر منفی داشت ولیکن باعث افزایش ارتفاع ویلی در منطقه دئودنوم گردید.
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