Effects of in ovo Injection of Zinc Acetate on some Gene Expression Associated with Embryonic Growth and Development, and with Growth and Carcass Characteristics of the Resultant Chicks
محورهای موضوعی : Camelم. تقی پور شه بندی 1 , ز. انصاری پیرسرائی 2 , ح. دلدار 3 , م. کاظمی فرد 4
1 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
4 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
کلید واژه: Zinc, chickens, growth and development, insulin-like growth factors, myogenic regulatory factors, <i>in ovo</i> injection,
چکیده مقاله :
This study was conducted in two steps to determine the effects of in ovo injection of zinc acetate (ZAC) on some gene expression associated with embryonic growth and development, and with growth and carcass characteristics of the resultant chicks. In the first step the effect of in ovo injectionofZAC on the expression of insulin-like growth factors (IGFs:IGF-I and IGF-I), myogenic regulatory factors (MRFs) and metallothionein (MT) genes was examined, and in the step two, the associations between genes expression with fertility, embryonic mortality, hatchability, body weight (BW) and relative organ weight on day of hatch, and body weight (BW) and feed conversion ratio (FCR) a week after hatching were investigated. Seven hundred twenty fertile eggs (n=720) were randomly divided into 4 treatment groups consisted of: 1) non-injected (NI) group, 2) sham group (group injected with deionized water (DW)), and 3) and 4) groups injected with 3.64 and 5.23 M ZAC, respectively. On day 3 of incubation the eggs were injected with 50 µL of each treatment into the albumen. On day 5 of the incubation and on hatch day, liver and breast muscle of the chicks were removed and immediately stored at -80 ˚C until RNA extraction and real time-PCR analysis. In ovo ZAC injection increased myogenic differentiation factor both in embryo and breast muscle. MT mRNA expression was also increased by ZAC injection. Hatchability was lower in ZAC groups and early embryonic mortality was higher in sham control (SHC) and ZAC groups. Injection of ZAC improved both BW and FCR during the first week of rearing, and increased the relative weights of liver and breast muscle. In conclusion, in ovo injection of ZAC increased some of gene expressions of tissues and BW7 during the first week, but decreased hatchability. Hence, lower doses of ZAC are recommended to use in further experiments.
در این پژوهش تأثیر تزریق درون تخم مرغی استات روی (ZAC)، بر بیان برخی از ژن های مرتبط با رشد و نمو جنینی و برخی از ویژگی های لاشه جوجه ها طی دو گامه بررسی شد. درگامه نخست تأثیر تزریق درون تخم مرغی ZAC، بر بیان نسبی ژن های فاکتورهای رشد انسولین مانند (IGF-I، IGF-II و IGF-IR)، فاکتورهای تنظیمی ماهیچه (MyoD و MYf-5) و متالوتیونین (MT) بررسی، و در گامه دوم ارتباط بین بیان ژن ها با باروری، مرگ و میر جنینی، جوجه دهی، وزن بدن (BW)، درصد وزن اجزای لاشه در روز تولد، وزن بدن (BW) و ضریب تبدیل غذایی (FCR) هفت روز پس از تولد بررسی شد. هفتصد و بیست تخم مرغ بارور (n=720) به طور تصادفی به چهار تیمار تقسیم شدند که در برگیرنده: 1) بدون تزریق(NI)، 2) تزریق آب بدون یون (DW)، 3) و 4) به ترتیب تزریق 3.64 و 5.23 مولار استات روی (به ترتیب ZAC 3.64 و ZAC 5.23) بود. در روز سه جوجه کشی، مقدار 50 میکرولیتر از هر تیمار در آلبومن تزریق شد. در روز پنج جوجه کشی و در روز تولد بافت جگر و ماهیچه سینه جوجه ها جدا شدند و فوری در 80- درجه سانتیگراد تا روز استخراج RNA و آنالیز real time-PCR ذخیره شدند. روی باعث افزایش بیان فاکتور تمایز مایوژنیک (MyoD) در بافت جنینی و ماهیچه شد. بیان mRNAی MT با تزریق ZAC افزایش یافت. یا درصد جوجه دهی در تیمارهای تزریق استات روی کمترین و مرگ و میر در گامه نخست جوجه کشی در تیمارهای ZAC و SHC (sham control) بیشترین بود. تزریق ZAC باعث بهبود BW و FCR در هفته نخست پرورش و افزایش درصد سینه و جگر شد.در نتیجه تزریق ZAC سبب افزایش بیان نسبی برخی از ژن های در برخی از بافت ها و BW7 طی هفته نخست شد اما درصد جوجه دهی کاهش یافت. بنابراین پیشنهاد می شود که در پژوهش های آینده از دوزهای کمتر استات روی استفاده شود.
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