Effects of in ovo Injection of Zinc Acetate on some Gene Expression Associated with Embryonic Growth and Development, and with Growth and Carcass Characteristics of the Resultant Chicks
الموضوعات :م. تقی پور شه بندی 1 , ز. انصاری پیرسرائی 2 , ح. دلدار 3 , م. کاظمی فرد 4
1 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
4 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
الکلمات المفتاحية: Zinc, chickens, growth and development, insulin-like growth factors, myogenic regulatory factors, <i>in ovo</i> injection,
ملخص المقالة :
This study was conducted in two steps to determine the effects of in ovo injection of zinc acetate (ZAC) on some gene expression associated with embryonic growth and development, and with growth and carcass characteristics of the resultant chicks. In the first step the effect of in ovo injectionofZAC on the expression of insulin-like growth factors (IGFs:IGF-I and IGF-I), myogenic regulatory factors (MRFs) and metallothionein (MT) genes was examined, and in the step two, the associations between genes expression with fertility, embryonic mortality, hatchability, body weight (BW) and relative organ weight on day of hatch, and body weight (BW) and feed conversion ratio (FCR) a week after hatching were investigated. Seven hundred twenty fertile eggs (n=720) were randomly divided into 4 treatment groups consisted of: 1) non-injected (NI) group, 2) sham group (group injected with deionized water (DW)), and 3) and 4) groups injected with 3.64 and 5.23 M ZAC, respectively. On day 3 of incubation the eggs were injected with 50 µL of each treatment into the albumen. On day 5 of the incubation and on hatch day, liver and breast muscle of the chicks were removed and immediately stored at -80 ˚C until RNA extraction and real time-PCR analysis. In ovo ZAC injection increased myogenic differentiation factor both in embryo and breast muscle. MT mRNA expression was also increased by ZAC injection. Hatchability was lower in ZAC groups and early embryonic mortality was higher in sham control (SHC) and ZAC groups. Injection of ZAC improved both BW and FCR during the first week of rearing, and increased the relative weights of liver and breast muscle. In conclusion, in ovo injection of ZAC increased some of gene expressions of tissues and BW7 during the first week, but decreased hatchability. Hence, lower doses of ZAC are recommended to use in further experiments.
Akshat G., Bhanja S.K., Mehra M. and Pande V. (2012). Does in ovo administration of zinc or iodine modulate differential of growth and immune related genes in broiler chickens. World,s Poult. Sci. J. 17, 1030-1034.
Andrews G.K. and Fernando L.P. (1991). Molecular biology of the chicken metallothionein gene. Pp. 103-120 in Metallothionein in Biology and Medicine. C.D. Klaassen and K.T. Suzuki, Eds., CRC Press, Boston, Massachusetts.
Batal A.B., Parr T.M. and Baker D.H. (2001). Zinc bioavailability in tetrabasic zinc chloride and dietary zinc requirement of young chicks fed a soy concentrate diet. Poult. Sci. 80, 87-90.
Blamberg D.L., Blackwood U.B., Supplee W.C. and Combs G.F. (1960). Effect of zinc deficiency in hens in hatchability and embryonic development. Proc. Soc. Exp. Biol. Med. 104, 217-220.
Caleb O., Molokwu B.S., Young V. and Li M. (2006). Zinc homeostasis and bone mineral density. Ohio Res. Clin. Rev. 15, 7-15.
Cao J., Henry P.R., Davis S.R., Cousins R.J., Miles R.D., Littell R.C. and Ammerman C.B. (2002). Relative bioavailability of organic zinc sources based on tissue zinc and metallothionein in chicks fed conventional dietary zinc concentrations. Anim. Feed Sci. Technol. 101, 161-170.
Chakraborty T., Maiti I.B. and Biswas B.B. (1987). A single form of metallothionein is present in both heavy metal induced and neonatal chicken liver. J. Biosci. 11, 379-390.
Christ B., Jacob H.J. and Jacob M. (1977). Experimental analysis of the origin of the wing musculature in avian embryos. Italian J. Anat. Embryol. 150, 171-186.
Cobb. (2001). Cobb Breeder Management Guide. WebMD. www.cobb-vantress.com/docs/. Accessed Jan. 2018.
Coolican S.A., Samuel D.S., Ewton D.Z., Mcwade F.J. and Florini J.R. (1997). The mitogenic and myogenic actions of insulin-like growth factors utilize distinct signaling pathways. J. Cell Biol. 272, 6653-6662.
Engert J.C., Berglund E.B. and Rosenthal N. (1996). Proliferation precedes differentiation in IGF-1-stimulated myogenesis. J. Cell Biol. 135, 431-440.
Ezzati M.S., Bozorgmehrifard M.H., Bijanzad P., Rasoulinezhad S., Moomivand H., Faramarzi S., Ghaedi A., Ghabel H. and Stabraghi E. (2013). Effect of different levels of zinc supplementation on broilers performance and immunity response to newcastle disease vaccine. European J. Exp. Biol. 3, 497-501.
Favero A., Vieira L., Angel C.R., Bess F., Cemin H.S. and Ward T.L. (2013). Reproductive performance of cobb 500 breeder hens fed diets supplemented with zinc, manganese, and copper from inorganic and amino acid- complexed sources. J. Appl. Poult. Res. 22, 80-91.
Fernandez A.M., Dupont J., Farrar R.P., Lee S., Stannard B. and Roith D. (2002). Muscle-specific inactivation of the IGF-1 receptor induces compensatory hyperplasia in skeletal muscle. J. Clin. Invest. 109, 347-355.
Fernando L.P., Wei D. and Andrews G.K. (1989). Structure and expression of chicken metallothionein. J. Nutr. 119, 309-318.
Fleet J.C. and Mccormick C.C. (1988). The ontogeny and induction by zinc of hepatic chick embryo metallothionein. Exp. Biol. Med. 188, 52-58.
Florini J.R., Ewton D.Z. and Coolican S.A. (1996). Growth hormone and the insulin-like growth factor system in myogenesis. Endocr. Rev. 17, 481-517.
Florini J.R., Magri K.A. and Ewton D.Z. (1991). Spontaneous differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. J. Biol. Chem. 266, 15917-15923.
Hamburger V. and Hamilton H.L. (1951). A series of normal stages in the development of the chick embryo. J. Morphol. 88, 49-92.
Hamza R.T., Hamed A.I. and Sallam M.T. (2012). Effect of zinc supplementation on growth hormone insulin growth factor axis in short Egyptian children with zinc deficiency. Italian J. Pediatr. 38, 8-21.
Hubbard S.R., Bishop W.R., Kirschmeier P., George S.J., Cramer S.P. and Hendrickson W.A. (1991). Identification and characterization of zinc binding sites in protein kinase C. Science. 254, 1776-1779.
Hudson B.P., Dozier W.A., Wilson J.L., Sander J.E. and Ward T.L. (2004). Reproductive performance and immune status of caged broiler breeder hens provided diets supplemented with either inorganic or organic source of zinc from hatching to 65 wk of age. J. Appl. Poult. Res. 13, 349-359.
Imamoglu S., Bereket A., Turan S., Taga Y. and Haklar G. (2005). Effect of zinc supplementation on growth hormone secretion, IGF-1, IGFBP3, somatomedian generation, alkalin phosphatase, osteocalcin and growth in prepubertal children with idiopathic short stature. J. Pediatr. Endocrinol. Metabol. 18, 69-74.
Joshua P.P., Valli C. and Balakrshnan V. (2016). Effect of in ovo supplementation of nano forms of zinc, copper, and selenium on post-hatch performance of broiler chicken. Vet. World. 9(3), 287-294.
Kielbowna L. and Jedrzejowska I. (2012). How is myogenesis initiated in chordates? Folia Biol. 60, 107-119.
Kienholz E.W., Turk D.E., Sybde Abdw M.L. and Hoekstra G. (1961). Effects of zinc deficiency in diets of hens. J. Nutr. 75, 211-221.
Kocamis H. and Killefer J. (2003). Expression profiles of IGF-I, IGF-II, bFGF and TGF-β2 growth factors during chicken embryonic development. Turkish J. Vet. Anim. 27, 367-372.
Kocamis H., Kirkpatrick-Keller D.C., Klandorf H. and Killefer J. (1998). In ovo administration of recombinant human insulin-like growth factor-1 alters postnatal growth and development of the broiler chicken. Poult. Sci. 77, 1913-1919.
Kucuk O., Sahin N. and Sahin K. (2003). Supplemental zinc and vitamin A can alleviate negative effects of heat stress in broiler chicken. Biol. Trase Elem. Res. 94, 225-236.
Lin-Jones J. and Hauschka S. (1996). Myogenic determination factor expression in the developing avian limb bud: an RT-PCR analysis. Dev. Biol. 174, 407-422.
Livak K.J. and Schmittgen T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25, 402-408.
Lu J.W., Mcmurtry J.P. and Coon C.N. (2007). Developmental changes of plasma insulin, glucagon, insulin-like growth factors, thyroid hormones, and glucose concentrations in chick embryos and hatched chicks. Poult. Sci. 86, 673-683.
MacDonald R.S. (2000). The role of zinc in growth and cell proliferation. J. Nutr. 130, 1500-1508.
Maret W. and Vallee B.L. (1998). Thiolate ligands in metallothionein confer redox activity on zinc clusters. Proc. Natl. Acad. Sci. USA. 95, 3478-3482.
Mccormick C.C. (1984). Induction and accumulation of metallothionein in liver and pancreas of chicks given oral zinc: A tissue comparison. J. Nutr. 114, 191-203.
Mccusker R.H. and Novakofski J. (2004). Zinc partitions IGFs from soluble IGF binding proteins (IGFBP)-5, but not soluble IGFBP-4, to myoblast IGF type 1 receptors. J. Endocrinol. 180, 227-246.
Musawi S.L., Lock F., Simbi B.S., Bayol S.A.M. and Stickland N.C. (2011). Muscle specific differences in the regulation of myogenic differentiation in chickens genetically selected for divergent growth rates. Differentiation. 82(3), 127-135.
Norouzi E., Daneshyar M. and Farhoomand P. (2013). Dietary supplementation effects of zinc acetate and magnesium sulfate on performance and antioxidant status of broilers under continuous heat stress. Spanish J. Agric. Res. 11, 127-131.
O’neill J. (1987). Growth and differentiation during myogenesis in the chick embryo. Dev. Biol. 120, 465-480.
Oh S.H., Nakaue H., Deagen J.T., Whanger P.D. and Arscott G.H. (1979). Accumulation and depletion of zinc in chick tissue metallothioneins. J. Nutr. 109, 1720-1731.
Olson E.N. (1992). Interplay between proliferation and differentiation within the myogenic lineage. Dev. Biol. 154, 261-272.
Parker M.H., Seale P. and Rudnicki M.A. (2003). Looking back to the embryo: Defining transcriptional networks in adult myogenesis. Nat. Rev. Genet. 4, 497-507.
Paulk C.B., Tokach M.D., Nelssen J.L., Burnett D.D., Vaughn M.A., Phelps K.J., Dritz S.S., Derouchey J.M., Goodband R.D., Houser T.A., Haydon K.D. and Gonzalez J.M. (2014). Effects of source and level of added zinc on muscle fiber diameter and skeletal muscle protein turnover of finishing pigs fed ractopamine HCl. J. Anim. Sci. 92(2), 132-141.
Petrie L., Buskin J.N. and Chesters J.K. (1996). Zinc and the initiation of myoblast differentiation. Nutr. Biochem. 7, 670-676.
Pownall M.E., Gustafsson M.K. and Emerson C.P. (2002). Myogenic regulatoryfactorsand the specification of muscle progenitors in vertebrate embryos. Ann. Rev. Cell. Dev. Biol. 18, 747-783.
Quinn L.S., Steinmetz B., Maas A., Ong A. and Kaleko M. (1994). Type-1 insulin-like growth factor receptor overexpression produces dual effects on myoblast proliferation and differentiation. J. Cell. Physiol. 159, 387-398.
Richards M.P. and Steele N.C. (1987). Trace element metabolism in the developing avian embryo. J. Exp. Zool. 1, 39-51.
Richter J.J., Killefer J., Kaczmarczyk W., Klandorf H., Kenney P.B. and Panaccione D. (2002). Gene expression during skeletal muscle development: Affect of in ovo IGF-1 administration on broiler embryogenesis and postnatal myogenesis in the mouse. Ph D. Thesis. University of West Virginia, USA.
Saenmahayak B., Singh M., Bilili S.F. and Hess J. B. (2012). Influence of dietary supplementation with complexed zinc on meat quality and shelf life of broilers. Int. J. Poult. Sci. 11, 28-32.
Sahin K., Onderci M., Sahin N., Gursu F., Yildiz N., Avci M. and Kucuk O. (2006). Responses of quail to dietary vitamin E and zinc picolinate at different environmental temperatures. Anim. Feed Sci. Technol. 129, 39-48.
Saitoh O., Fujisawa-Sehara A., Nabeshima Y. and Periasamy M. (1993). Expression of myogenic factors in denervated chicken breast muscle: Isolation of the chicken Myf5 gene. Nucleic Acids. Res. 21, 2503-2509.
Sandrock B.C, Kem S.R. and Bryan S.R. (1983). The movement of zinc and copper from the fertilized egg into metallothionein-like proteins in the developing chick hepatic tissue. Biol. Trace Elem. Res. 5, 503-515.
SAS Institute. (2002). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Savage J.E. (1968). Trace minerals and avian reproduction. Fed. Proc. 27, 927-931.
Shen S.F., Wang R.L., Lu L., Li S.F., Liu S.B., Xie J.J., Zhang L.Y., Wang M.L. and Luo X.G. (2013). Effect of intravenously injected zinc on tissue zinc and metallothionein gene expression of broilers. Br. Poult. Sci. 54(3), 381-390.
Shyam Sunder G., Vijay Kumar C., Panda A.K., Raju M.V.L.N. and Rama Rao V. (2013). Effect of supplemental organic Zn and Mn on broiler performance, bone measures, tissue mineral uptake and immune response at 35 days of age. Curr. Res. Poult. Sci. 3, 1-11.
Smith J. H. (1963). Relation of body size to muscle cell size and number in the chicken. Poult. Sci. 42, 283-290.
Stanley V., Hickerson G.K., Oaley M.B., Hume M. and Hinton A. (2012). Single and combined effects of organic selenium and zinc on eggs fertility, hatchability, and embryonic mortality of Cochin hens. Agrotechnology. 2, 1-3.
Tako E.P., Ferket R. and Uni Z. (2005). Changes in chicken intestinal zinc exporter mRNA expression and small intestinal functionality following intra-amniotic zinc-methionine administration. J. Nutr. Biochem. 16, 339-346.
Velleman S.G. (2007). Muscle development in the embryo and hatchling. Poult. Sci. 86, 1050-1055.
Wang X., Fosmire G.J., Gay C.V. and Leach R.M. (2002). Short-term zinc deficiency inhibits chondrocyte proliferation and induces cell apoptosis in the epiphyseal growth plate of young chickens. J. Nutr. 132, 665-673.
Wilsons H.R. (1997). Effects of maternal nutrition on hatchability. Poult. Sci. 76, 134-143.
Yu Z.P., Le G.W. and Shi Y.H. (2005). Effect of zinc sulphate and zinc methionine on growth, plasma growth hormone concentration, growth hormone receptor and insulin-like growth factor-1 gene expression in mice. Clin. Exp. Pharmacol. Physiol. 32, 273-278.
