Estimation of Inbreeding Coefficients Using Pedigree and Microsatellite Markers and Its Effects on Economic Traits of Shirvan Kordi Sheep
محورهای موضوعی : Camelس. نقویان 1 , س. حسنی 2 , م. آهنی آذری 3 , ع.ر. خان احمدی 4 , د.ع. ساقی 5 , ن. مامیزاده 6
1 - Department of Animal and Poultry Genetics, Breeding and Physiology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 - Department of Animal and Poultry Genetics, Breeding and Physiology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
3 - Department of Animal and Poultry Genetics, Breeding and Physiology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
4 - Department of Animal Science, Gonbad University, Gonbad, Iran
5 - Mashad Agriculture and Natural Resources Research Center, Mashad, Iran
6 - Department of Animal and Poultry Genetics, Breeding and Physiology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
کلید واژه: Sheep, growth traits, inbreeding, genetic markers,
چکیده مقاله :
In this study, pedigree data of 7170 lambs produced from 177 rams and 2182 ewes collected during 1989 to 2009 in the Shirvan Kordi sheep breeding station, were used for calculating inbreeding coefficients. The impact of inbreeding on the studied traits was investigated by including inbreeding coefficients for each animal as a covariate in the animal model. Average inbreeding coefficient of the flock in base year, 1989, was estimated as 0.668%. The low average inbreeding in the flock could be ascribed to the structured breeding regime of the station for preventing mating among relatives. Totally, 23.3% of the animals were inbred with average inbreeding coefficient of 2.87%. Out of these, 1668 animals were inbred including 823 males and 845 females with the mean inbreeding coefficients of 2.81% and 2.93%, respectively. Coefficients of inbreeding were also estimated in this population using 6 microsatellite markers (BM8125, BMS2361, BM6526, BM6526, BMS1004 and BM6444) and 100 blood samples. Within population, inbreeding F-statistic (FIS) ranged from -0.1859 (BM6526) to 0.3329 (BM6438). The FIS estimates were positive for BM6438 and BM6444 loci (0.3329 and 0.2287), respectively and estimates for the other loci were negative. Average inbreeding coefficients based on molecular markers was estimated as 0.2617%. Birth weight, weaning weight, body weight at 6 months, body weight at 9 months, body weight at 12 months, annual wool production and number of lambs per ewe lambing were changed by -0.0131, 0.0795, 0.0013, -0.0653, -0.0921, 0.0083 and -0.023 kg, respectively for every one percent increase in inbreeding.
در این پژوه، میزان همخونی 7170 رأس بره حاصل از 177 قوچ و 2182 میش که در طی سال‌های 1388-1368 از گله گوسفند کردی ایستگاه پرورش و اصلاحنژاد شیروان جمع‌آوری شده بود، مورد بررسی قرار گرفت. اثر همخونی بر صفات مورد مطالعه با مدل حیوانی که در آن ضریب همخونی حیوان به عنوان متغیر کمکی در نظر گرفته شده بود، بررسی شد. میانگین ضریب همخونی بر اساس سال پایه 1368، 668/0 درصد برآورد شد. میانگین کم ضریب همخونی در گله را میتوان به ساختار پرورش ایستگاه و تلاش مسئولین ایستگاه برای کنترل همخونی و جلوگیری از آمیزش‌‌های خویشاوندی نسبت داد. از کل شجره تحت مطالعه 3/23 درصد حیوانات، همخون بودند و میانگین ضریب همخونی آنها 87/2 درصد بود. از 1668 رأس حیوان همخون، 823 رأس نر و 845 رأس ماده بودند و میانگین ضریب همخونی آنها بهترتیب 81/2 و 93/2 درصد بود. برآورد ضریب همخونی در جمعیت گوسفند کردی با استفاده از 6 جایگاه ریزماهواره‌ای (BM8125، BMS2361، BM6526، BM6438، BMS1004 و BM6444) و 100 نمونه خون انجام گرفت. ضریب همخونی بر اساس شاخص رایت (FIS) در جمعیت گوسفندان کردی ایستگاه پرورش و اصلاح نژاد شیروان، از 1859/0- برای جایگاه BM6526 تا 3329/0 در جایگاه BM6438 متغیر بود. در جایگاه‌های BM6438 و BM6444 مقدار شاخص FIS، مثبت بود و بهترتیب 3329/0 و 2287/0 برآورد شد و برای سایر جایگاهها منفی بود. میانگین ضریب همخونی بر اساس داده‌های مولکولی، 2617/0 درصد به دست آمد. صفات وزن‌های تولد، شیرگیری، شش ماهگی، نه ماهگی، یک سالگی، تولید پشم سالیانه (در اولین پشمچینی) و تعداد بره‌ها در هر زایش میش (اولین زایش) به ازای افزایش یک درصد همخونی به ترتیب 0131/0-، 0795/0، 0013/0، 0653/0-، 0921/0- و 0083/0 کیلوگرم و 023/0- رأس تغییر نمودند.
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