The effects of hydro-alcohol extract of follower of marshmallow (Althaea officinalis L.) on some biochemical and hematological parameters in common carp (Cyprinus carpio L.)
محورهای موضوعی : مجله گیاهان داروییفهیمه فلاح پور 1 , مهدی بنایی 2 , نرگس جوادزاده 3
1 - گروه شیلات، پردیس علوم و تحقیقات خوزستان، دانشگاه آزاد اسلامی، اهواز، ایران
2 - گروه شیلات، دانشکده منابع طبیعی و محیط زیست، دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، بهبهان، ایران
3 - گروه شیلات، پردیس علوم و تحقیقات خوزستان، دانشگاه آزاد اسلامی، اهواز، ایران
کلید واژه: Common carp, Biochemical parameters, Blood cells, Marshmallow extract, Althaea officinalis L,
چکیده مقاله :
Background & Aim: Marshmallow (Althaea officinalis) is one of the most popular medicinal plants used in traditional medicine with antibacterial and antioxidant properties. This study was conducted to evaluate the effects of marshmallow extract (Althaea officinalis L.) administration on blood cells and biochemical parameters of carp liver. Experimental: 150 carp (weighing 37.65 ± 4.40 g) were fed with diets containing 0.0 (control diet), 2.5, 5, and 10 g marshmallow extract for 60 days. Then, red blood cell indices (RBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCH), white blood cells differential count and biochemical parameters of liver such as aspartate aminotransferase (AST), alanine amino transferase (ALT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) were measured on days 30 and 60 of the experiment. Results & Discussion: On days 30 and 60 of the experiment, red blood cell indices (RBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCH), white blood cells differential count and biochemical parameters of liver such as aspartate aminotransferase (AST), alanine amino transferase (ALT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) were measured. On day 60, no significant difference was found (p>0.05) in RBC, Hb, Htc, MCV, MCH count, number of neutrophils, basophils, and eosophils in fish fed with different concentrations of marshmallow extract. Administration of marshmallow extract (5 g) significantly increased (p<0.05) WBC and lymphocytes on day 60. On the other hand, administration with 5 and 10 g of marshmallow extract decreased monocytes (p<0.05) on day 30. The significant increase was observed in AST, ALT, ALP and LDH levels in liver of fish fed with 10 g of marshmallow extract may be attributed to cytotoxicity, however 2.5 and 5 g of extract did not have adverse effects on liver enzymes. Industrial and practical recommendations: According to the results, it was concluded that preclinical administration of certain concentrations of marshmallow extract (2.5 and 5 g) was beneficial for carp.
مقدمه و هدف: گیاه ختمی (Althae officinalis L.) یکی از معروفترین گیاهان دارویی است که به دلیل داشتن خواص ضد باکتریایی و آنتیاکسیدانی در طب سنتی مورد استفاده قرار میگیرد. هدف از انجام این مطالعه، بررسی تاثیر تجویز عصاره گل ختمی بر پارامترهای خونی و بیوشیمیایی در ماهی کپور است. روش تحقیق:در این آزمایش 150 عدد ماهی کپور معمولی (با میانگین وزنی 40/4±65/37 ) با جیرههای حاوی 0/0 (کنترل)، 5/2، 5 و 10 گرم عصاره گل ختمی به مدت 60 روز تغذیه شدند. سپس در روزهای 30 و 60 آزمایش، شاخصهای خونشناسی و بیوشیمیایی ماهیها اندازهگیری شد. نتایج و بحث: هیچ گونه تغییر معنیداری در تعداد سلول قرمز خون، هموگلوبین، هماتوکریت و میانگین حجم گویچهها، تعداد نوتروفیلها، بازوفیلها و ائوزینوفیلها در ماهیانی که با غلظتهای مختلف عصاره گل ختمی به مدت 60 روز تغذیه شدند، مشاهده نگردید. تجویز عصاره ختمی (5 گرم) موجب افزایش معنیداری در تعداد سلولهای سفید خون و لنفوسیتها در روز 60 شد. از سویی دیگر تجویز 5 و 10 گرم عصاره ختمی سبب کاهش معنیدار منوسیتها گردید. تجویز عصاره ختمی در طی 60 روز تاثیر معنیداری بر فعالیت آنزیمهای آلانین آمینوترانسفراز، آلکالین فسفاتاز و کراتینفسفوکیناز و نیز سطح کراتینین خون نداشت. فعالیت آنزیم آسپارتات آیمنوترانسفراز و لاکتات دهیدروژناز پلاسما و همچنین سطح پروتئین کل پلاسما و آلبومین به طور معنیداری در ماهیان تحت تیمار غلظت 10 گرم عصاره ختمی در بالاترین سطح قرار داشت. سح کلسترول و تریگلیسرید نیز در گروههای تحت تیمار غلظتهای 5 و 10 گرم عصاره ختمی به طور معنیداری کاهش یافت. تغییرات معنیداری مشاهده شده در فاکتورهای خونی و بیوشیمیایی ماهیان تحت تیمار غلظت 10 گرم عصاره ختمی ممکن است به سمیت سلولی نسبت داد؛ در حالی که تجویز 5/2 و 5 گرم عصاره ختمی تاثیر سوئی بر شاخصهای خونی و بیوشیمیایی ماهیان کپور در این مطالعه نداشت. توصیه کاربردی/ صنعتی:براساس این نتایج، تجویز پیشبالینی غلظتهای مشخصی از عصاره گل ختمی (5/2 و 5 گرم) برای ماهیان کپور معمولی مفید بوده است.
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