Analgesic and anti-inflammatory activities of Zea mays leaves

محورهای موضوعی : مجله گیاهان دارویی

جود اکوکون ¹ (Department of pharmacology and toxicology, Faculty of pharmacy, University of Uyo, Uyo, Nigeria)
کوفره دیویز ² (Department of physiology, Faculty of basic medical sciences, University of Uyo, Uyo, Nigeria)
باسی آنتیا ³ (Department of chemistry, University of Uyo, Uyo, Nigeria)

تاریخ دریافت : 1394/01/30 تاریخ پذیرش : 1394/12/01 تاریخ انتشار : 1395/04/11

کلید واژه: Zea mays, GC-MS, Anti-inflammatory, analgesic,

چکیده مقاله :

Background & Aim:Zea mays leaf used traditionally in Ibibio ethno medicine in Southern Nigeria to treat malaria and other inflammatory diseases. These anti-inflammatory and analgesic properties were evaluated against experimentally induced inflammation and pains using standard models. Experimental: The effects of ethanol leaf extract of Zea mays (170 -510 mg/kg) on experimentally induced inflammation and pain were studied in rats using Carrageenin, egg albumin and xylene-induced inflammation as well as acetic acid-induced writhing and thermal induced pain models. The GCMS analysis of the most active fraction was carried out. Results: The extract (170 -520 mg/kg) inhibited inflammation and pains caused by different phlogistic agents used in a dose dependent fashion. The various degrees of inhibitions were statistically significant (p<0.05, 0.01, 0.001), though incomparable to that of the standard drugs used (ASA and indomethacin). The GC-MS revealed the present of polyunsaturated fatty acids and phenolic compounds which may be responsible for the observed activities of the extract. Recommended applications/industries: The present study demonstrates that leaf extract of Zea mays might to be useful for the treatment of inflammation and pains.

چکیده انگلیسی:

Background & Aim:Zea mays leaf used traditionally in Ibibio ethno medicine in Southern Nigeria to treat malaria and other inflammatory diseases. These anti-inflammatory and analgesic properties were evaluated against experimentally induced inflammation and pains using standard models. Experimental: The effects of ethanol leaf extract of Zea mays (170 -510 mg/kg) on experimentally induced inflammation and pain were studied in rats using Carrageenin, egg albumin  and xylene-induced inflammation as well as acetic acid-induced writhing and thermal induced pain models. The GCMS analysis of the most active fraction was carried out. Results: The extract (170 -520 mg/kg) inhibited inflammation and pains caused by different phlogistic agents used in a dose dependent fashion. The various degrees of inhibitions were statistically significant (p<0.05, 0.01, 0.001), though incomparable to that of the standard drugs used (ASA and indomethacin). The GC-MS revealed the present of polyunsaturated fatty acids and phenolic compounds which may be responsible for the observed activities of the extract. Recommended applications/industries: The present study demonstrates that leaf extract of Zea mays might to be useful for the treatment of inflammation and pains.

منابع و مأخذ:

Abo, K.A., Fred-Jaiyesimi, A.A., Jaiyesimi, A.E.A. 2008. Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in South Western Nigeria. J Ethnopharmacol., IS: 67-71.

Adams, R.P. 2001. Identification of Essential oils by Gas Chromatography Quadrupole Mass Spectrometry. Allured Publishing Corporation, Carol Stream, USA

Ahmed, M., Sadhu, S.K., Datta, B.K., Kunu, J.K., Bachar, S.C. 1997. Preliminary studies on the antiinflammatory, analgesic and diuretic activity of stagninol, a sesquiterpene isolated from Persicaria stagnina. Pharmazie., 52(6):472-475.

Akah, P.A., Njike, H.A. 1990. Some pharmacological effects of rhizome aqueous extract of Anchomanes diformis. Fitoterapia., 60: 368-370.                                                   

Akah, P.A., Nwanbie, A.  1994. Evaluation of Nigerian traditional medicines plants used for rheumatic (inflammatory) disorder. Journal of Ethnopharmacology., 42: 179 – 182.

Alamed, J., Chaiyasit, W., McClements, D.J., Decker, E.A. 2009. Relationships between free radical scavenging and antioxidant activity in foods. Journal of Agricultural and  Food Chemistry., 57: 2969-2976.

Amico-Roxas, M., Caruso, A., Trombadore, S., Scifo, R., Scapagnime, U. 1984. Gangliosides antinociceptive effects in rodents. Archieves of International Pharmacodynamics and Therapeutics., 272: 103-117.

Balasubramanian, K., Padma, P.R. 2013. Anticancer activity of Zea mays leaf extracts on oxidative stress-induced Hep2 Cells. J Acupuncture and Meridian Studies., 6(3):149-158.

Balasubramanian, K., Padma, P.R. 2012. Screening of Antioxidant Properties of Zea mays Leaves at different time periods of growth. Journal of Pharmacy Research., 5(8):4034-4037.

Balasubramanian, K., Vidhya, A., Thiruselvi, M., Sudhadevi,   M. and Padma, P.R. 2014. Zea mays leaf extracts exhibits anticancer property and enhance the chemotherapeutic action of etoposide in cancer cells. Indo American Journal of Pharmaceutical Research., 4(03): 1530 - 1539.

 Balasubramanian, K., Jincy, P.A., Padma, P.R. 2015. Influence of methanolic extract of Zea mays leaves against CCL₄ and H₂O₂ induced oxidative stress in Drosophila melanogaster. Indo American Journal of Pharmaceutical Research., 5(01): 566-577.

Bentley, G.A., Newton, S.H., Starr, J. 1983. Studies on the antinociceptive action of agonist drugs and their interaction with opoid mechanisms. British  Journal of Pharmacy., 79: 125 - 134.

Besra, S.E., Sharma, R.M., Gomes, A. 1996. Antiinflammatory effect of petroleum ether extract of leaves of Litchi Chinensis. Caertn (sapindaceae). Journal of Ethnopharmacology., 54:1-6.

Carlo Di, G., Mascolo, N., Izzo, A.A., Capasso, F. 1999. Flavonoids, old and new aspects of a class of natural therapeutic drugs. Life Science., 65: 337–353.

Correa, C.R., Kyle, D.J., Chakravarty, S., Calixto, J.B. 1996. Antinociceptive profile or the pseudopeptide β₂ bradykinin receptors antagonist NPC 18688 in mice. British Journal of Pharmacology., 117: 552-556.

Deraedt, R,  Jougney, S.,  Falhout, M. 1980. Release of Prostaglandin E and F in an algogenic reaction and its inhibition.  European Journal of Pharmacy., 51: 17-24.

Dong, J., Cai, L., Zhu, X., Huang, X., Yin, T., Fang, H., Ding, Z. 2014. Antioxidant Activities and Phenolic Compounds of Cornhusk, Corncob and Stigma Maydis. J. Braz. Chem. Soc., 25(11): 1956-1964.

      Ekpendu, T.O., Akah, P.A., Adesomoju, A.A., Okogun, J.I. 1994. Antinflammatory and antimicrobial activities of Mitracarpus scaber extracts. International Journal of Pharmacology., 32:191-195.

Foster S, Duke JA. 1990. Field Guide 10 Medical Plants: Eastern and Central North America. Houghton MifAin, Boston.

Gill LS. 1992. Ethnomedical Uses of Plants in Nigeria. Uniben Press, Benin, Nigeria, p. 249.

Franzotti EM, Santos CVF, Rodrigues HMSL, Mourao RHV, Andrade MR, Antoniolli AR. 2002. Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. Journal of Ethnopharmacology., 72: 273-278.

Hess SM, Milonig RC. 1972. Inflammation In: Lepow LH, Ward PS. (Eds). Inflammation, Mechanism and control. Academic Press, New-York, USA. pp.1-2.

 

Huss U, Ringbom T, Perera P, Bohlin L, Vasange M. 2002. Screening of ubiquitous plant constituents for COX-2 inhibition with a scintillation proximity based assay. Journal Natural Products., 65: 1517-1521. 

Krogh R, Kroth R, Berti C, Madereira AO, Souza MM, Cechinel-Filho V, Delle-Monache F, Yunes RA. 1999. Isolation and identification of compounds with antinociceptive action from Ipomoea pes-caprae. Pharmazie., 54: 464 – 466.

Kumar PP, Kumaravel S, Lalitha C. 2010. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res., 4 (7): 191-195.

Liang YC, Huang YT, Tsau SH, Lin-Shiau SY, Chen CF, Lin JK. 1999. Suppression of inducible cyclo-oxygenase and inducible nitric acid synthase by apigenia and related flavonoid in mouse macrophages. Carcinogenesis., 20: 1945-52.

Ledón N, Casacó  A, Rodríguez  V, Cruz J, González R, Tolón  Z, Cano M, Rojas E.2003. Anti-Inflammatory and Analgesic Effects of a Mixture of Fatty acids isolated and purified from sugar cane wax oil. Planta Medica., 69(4): 367-369

Li, R.W., Leach, D.N., Myers, P., Leach, G.J., Lin, G.D., Brushett, D.J., Waterman, P.G. 2004. Anti-inflammatory activity, cytotoxicity and active compounds of Tinospora smilacina Benth. Phytotherapy Reserch., 18: 78-83.

Lin, L.L., Lin, A.Y., Knopt, J.L. 1992. Cytosolic phospholipase A₂ is coupled to hormonally regulated release of arachidonic acid. Proceeding of National Academy of Science, U. S. A., 89: 6147-6157.

Liu, J. 1995. Pharmacology of oleanolic acid and ursolic acid. Journal of Ethnopharmacology., 49: 57- 68.

Lorke, D. 1983. A new approach to practical acute toxicity testing. Archieves of Toxicology., 54:275-286.

Maia, J.L., Lima-Junior, R.C., David, J.P., David, J.M., Santos, F.A., Rao, V.S. 2006. Oleanolic acid, a pentacyclic triterpene attenuates the mustard oil induced colonic nociception in mice. Biological and Pharmaceutical Bulletin., 29: 82–85.

Middleton, E Jr, Kandaswami, C., Theoharides, T.C. 2000. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacological Reviews., 52: 673–751.

Nwafor, P.A., Jacks, T.W., Ekanem, A.U. 2007. Analgesic and anti-inflammatory effects of methanolic extract of Pausinystalia mecroceras stem bark in rodents. Journal of  Pharmacology., 3:86-90.  

Nwafor, P.A., Nwajiobi, N., Uko, I.E., Obot, J.S. 2010. Analgesic and anti-inflammatory activities of an ethanol extract of Smilax krausiana leaf in mice. African Journal of Biomedical and Research., 13: 141 -148.

Osagie, A.U., Eka, O.U.1998. Nutritional quality of plant foods.Post Harvest Research Unit, University of Benin, Benin, Nigeria, pp 34-41.

Okokon, J.E., Nwafor, P.A. 2010. Antiinflammatory, analgesic and antipyretic activities of ethanolic root extract of Croton zambesicus. Pakistan Journal of Pharmaceutical sciences., 23(4): 383 - 390.

Owoyele, B.V., Negedu, M.N., Olaniran, S.O.,  Onasanwo, S.A., Oguntoye, S.O., Sanya, J.O., Oyeleke, S.A., Ibidapo, A.J., Soladoyel, A.O. 2010. Analgesic and anti-inflammatory effects of aqueous extract of Zea mays husk in male wistar rats. Journal of Medicinal Food., 13 (2):343-347.

Oriowo, M.A. 1982. Anti-inflammatory activity of piperonyl,-4-acrylic isobutyl amide, an extractive from Zanthoxylum zanthoxyloids. Planta Medica., 44: 54 - 56

Otuki, M.F., Ferreira, J., Lima, F.V., Meyre-Silva, C., Malheiros, Â., Muller, L.A., Cani, G.S., Santos, A.R.S., Yunes, R.A., Calixto, J.B. 2005. Antinociceptive properties of mixture of α-amyrin and β-amyrin triterpenes: Evidence for participation of protein kinase C and protein kinase A pathways. Journal of Pharmacology and Experimental Therapeutics., 313: 310–318.

Raja Rajeswari, N., Rama Lakshmi, S., Muthuchelian, K. 2011. GC-MS Analysis of bioactive components from the ethanolic leaf extract of Canthium dicoccum (Gaertn.) Teijsm & Binn. Journal of Chemical and Pharmaceutical Research., 3(3):792-798

Rajendran, N.N., Thirugnanasambandam, P., Viswanathan, S., Parvathavarthini, S., Ramaswamy, S. 2000. Antinociceptive pattern of flavone and its mechanism as tested by formalin assay. Indian Journal of Experimental Biologiocal., 38: 182–185.

Ringbom, T., Segura, L., Noreen, Y., Perera, P., Bohlin, L. 1998. Ursolic acid from Plantago major, a selective inhibitor of cyclooxygenase-2 catalysed prostaglandin biosynthesis. Journal of Natural Products., 61: 1212–1215.

Robak, J., Shridi, F., Wolbis, M., Krolikowska, M. 1998. Screening of the influence of flavonoids on lipoxygenase and cyclooxygenase activity, as well as on nonenzymic lipid oxidation. Polish Journal of Pharmacology and Pharmacy., 40: 451–458.

Sokeng, S.D., Koubé, J., Dongmo, F., Sonnhaffouo, S., Nkono, B.L.N.Y., Taïwé, G.S., Cherrah, Y., Kamtchouing, P. 2013. Acute and chronic anti-inflammatory effects of the aqueous extract of Acacia nilotica (L.) Del. (Fabaceae) pods. Academia Journal of Medicinal Plants., 1: 1-5.

Santos, A.R., Cechinel Filho, V., Niero, R., Viana, A.M., Moreno, F.N., Campos, M.M., Yunes, R.A., Calixto, J.B. 1994. Analgesic effects of callus culture from selected species of Phyllanthus. Journal of Pharmacy and Pharmacology., 46 : 755 – 759.

Setzer, W.N., Stokes, S.L., Penton, A.F., Takaku, S., Haber, W.A., Hansell, E., Caffrey, C.R., McKerrow, J.H. 2007. Cruzain Inhibitory activity of leaf essential oils of neotropical lauraceae and essential oil components. Nat Prod Comm., 2(12): 1203–1210.

Simmonds, N.W. 1979. Evolution of Crop Plants. Longman. London.  pp. 128-129.

Sofowora, A. 1993.Medicinal Plants and Traditional Medicine in Africa. 2nd edn, Spectrum Book Ltd, Ibadan, Nigeria.

Suh, H.W., Song, D.K., Son, K.H., Wie, M.B., Lee, K.H., Jung, K.Y., Do, J.C., Kim, Y.H. 1996. Antinociceptive mechanisms of dipsacus saponin C administered intracerebroventricularly in the mouse. General Pharmacology., 27: 1167–1172.

Suh, N., Honda, T., Finaly, H.J., Barchowsky, A., Williams, C., Benoit, N.E. 1998. Novel triterpenoids suppress inducible nitric oxide synthase (iNOS) and inducible cyclooxygenase (COX-2) in mouse macrophages. Cancer Resesearch., 58: 717-723.

Trease, G.E., Evans, W. C. 1989. Pharmacognosy, 13^th ed. Bailliere Tindal, London.

Tapondjou, L.A., Lontsi, D., Sondengam, B.L., Choi, J., Lee, K.T., Jung, H.J., Park, H.J. 2003. In vivo antinociceptive and antiinflammatory effect of the two triterpenes, ursolic acid and 23-hydroxyursolic acid, from Cussonia bancoensis. Archives of Pharmacal Research., 26: 143–146.

Tjolsen, A., Berge, O.G., Hunskaar, S., Rosland, J.H., Hole, K. 1992. The formalin test: An evaluation of the method. Pain.,51:5-17.

Turner, R.A. 1995. Screening methods in Pharmacology. Vol 1. Academic Press. New York. Pp. 85- 106.

Vane, T., Booting, R. 1987. Inflammation and mechanism of action of anti- inflammatory drugs. FASSEB J., 1:89-96.

Vaz, Z.R., Cechinel, V., Yunes, R.A., Calixto, J.B. 1996. Antinociceptive action of 2-(4-bromobenzoyl)-3-methyl-4-6-dimethoxy bezofuran, a novel xanthoxyline derivative of chemical and thermal models of nociception in mice. Journal of Pharmacy and Experimental Therapeutics., 278: 304 - 312.

Winter, C.A., Risley, E.A., Nuss, G.W. 1962. Carrageenin-induced oedema in hind paw of the rats as an assay of anti-inflammatory drugs. Proceeding of Society for Experimental Biology Medicine., 111:544 -547.