بیوسنتز نانوذرات نقره توسط عصاره مریم گلی و ارزیابی فعالیت آنتی اکسیدانی و ضد میکروبی آن ها علیه باکتری های فساد غذایی
محورهای موضوعی :
بیوتکنولوژی و میکروبیولوژی موادغذایی
بهاره حاجی رستملو
1
(1- استادیار، گروه علوم و صنایع غذایی، واحد نیشابور ، دانشگاه آزاد اسلامی، نیشابور ، ایران)
راحله ژیانی
2
(2- دانشیار، گروه شیمی، واحد نیشابور ، دانشگاه آزاد اسلامی، نیشابور، ایران)
شکوفه عمرانی
3
(3- دانشجوی دکترای شیمی تجزیه، دانشگاه آزاد اسلامی، واحد نیشابور، باشگاه پژوهشگران جوان و نخبگان، نیشابور،)
تاریخ دریافت : 1396/08/13
تاریخ پذیرش : 1397/02/08
تاریخ انتشار : 1398/04/01
کلید واژه:
فعالیت آنتی اکسیدانی,
نانوذرات نقره,
فعالیت ضد میکروبی,
گیاه مریم گلی,
بیوسنتز,
چکیده مقاله :
هدف از مطالعه حاضر سنتز زیستی نانوذرات نقره با استفاده از عصاره آبی برگ گیاه مریم گلی و بررسی فعالیت آنتی اکسیدانی و ضد میکروبی آنها می باشد. برای سنتز نانوذرات نقره، عصاره آبی گیاه تهیه و به محلول نیترات نقره با غلظت 1 میلی مولار اضافه شد. فعالیت آنتی اکسیدانی نانو ذره نقره تولید شده با استفاده از قابلیت جذب رادیکال های 1،1-دیفنیل-2-پیکریل-هیدرازیل[1](DPPH) سنجش و فعالیت ضدمیکروبی نانوذرات نیز، به روش تعیین حداقل غلظت مهارکنندگی[2](MIC)، علیه سه باکتریE. coli O157:H7 (ATCC 43895), L. monocytogenes (ATCC 7644), S. typhimurium (ATCC 35987) مورد بررسی قرار گرفت. نانوذرات نقره سنتز شده بیشترین جذب را در430 نانومتر نشان دادند و دارای شکل کروی و میانگین اندازه آنها بین 50-30 نانومتر بودند. در این مطالعه نانو ذره نقره تولید شده به عنوان مهارکننده رادیکال DPPH(IC50 =170 μM) عمل کردند. مقادیر MIC نانو ذرات نقره در برابر پاتوژن های آزمایشی در محدوده 25/6- 12/3 میکرو گرم بر میلی لیتر بود. پاتوژن monocytogenes بالاترین میزان MIC را نشان داد. نتایج حاصل نشانگر فعالیت آنتی اکسیدانی و ضدمیکروبی مناسب نانوذرات بیوسنتز شده بودند. [1].1,1-diphenyl-2-picrylhydrazyl [2].Minimum Inhibition concentration
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