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Open Access Article
1 - Evaluation of bio removal nitrate by Thiobacillus denitrificance in the presence of quantum dot carbon stabilized zero valent iron nanoparticles (CQD-Fe0)
elahe hamdi behnam rasekh elahe tajbakhsh fatemeh yazdian maryam ghobehContamination of groundwater and surface water with nitrate has become a serious problem in many parts of the world. Adverse effects on aquatic ecosystems are one of the most important effects of high nitrate concentrations in aqueous solutions. Zero-valent iron (Fe 0) MoreContamination of groundwater and surface water with nitrate has become a serious problem in many parts of the world. Adverse effects on aquatic ecosystems are one of the most important effects of high nitrate concentrations in aqueous solutions. Zero-valent iron (Fe 0) metal nanoparticles are of great interest for the purification of toxic compounds from water. The aim of this study was to use nanostructure coated with carbon-quantum dot (CQD-Fe0) to investigate their role in biodenitrification of Thiobacillus denitrificance. For this purpose, Fe0 nanoparticles were synthesized by the liquid phase reduction method and for biocompatibility, uniform distribution and non-agglomeration, Fe0 was coated with carbon quantum-dot. Characterization of nanoparticles determined by XRD, TEM, FESEM, FTIR and DLS. The FTIR spectrum confirmed the formation of CQD and Fe0 bonds. The average diameter of CQD-Fe0 nanoparticles was observed in the range of 29.31-38.32. According to the results, with increasing temperature, growth and bio-nitrification activity of Thiobacillus denitrificance microorganism increases. Manuscript profile -
Open Access Article
2 - Preparation and formulation of low-calorie hot chili pepper sauce based on sugar beet molasses sweetener
ayda golbaaz alireza rahman Fateme hoseinmardiBecause of the high amount of waste produced by the food industry, this waste management has been considered by researchers. Molasses is a by-product of the production of crystal sugar, despite its functional and nutrient compounds, it is mostly used for livestock consu MoreBecause of the high amount of waste produced by the food industry, this waste management has been considered by researchers. Molasses is a by-product of the production of crystal sugar, despite its functional and nutrient compounds, it is mostly used for livestock consumption. This study aimed to use molasses in hot chili sauce formulation as a substitute for sucrose. The sugar beet molasses replaced sucrose in hot red pepper sauce formulation after heating as zero to 3%. pH, viscosity, moisture, calories value, Microbial (Mold and yeast), were studied, and sensory evaluation was measured by the five-point hedonic method. The results showed that an increase of sucrose in formulation, pH value, calories, microbial growth population decreased (p<0.05). Increased moisture content, and a* index observed in the treatments (P<0.05), The use of molasses up to 0.5% in the treatment had very taste favorable for the assessors. The treatment's texture was affected by 1% molasses replacement. The brittleness of the treatments changed and became a pasted. The most desirable treatment was it containing 0.25 and 0.5% molasses. According to the results, when molasses using in the sauce formulation, the quality, shelf life, and nutritional value of products can be improved. Manuscript profile -
Open Access Article
3 - Phenotypic and molecular detection of AmpC-beta-lactamase in Acintobacter baumannii strains isolated of clinical specimens
Masoumeh Ali Barari Fatemeh Noorbakhsh Sahar Honarmand JahromiBackground and aim: Acinetobacter baumannii is an opportunistic pathogen that remains persistent in the environment for a long time. The eradication of this bacteria is difficult, since it has the ability to acquire antibiotic resistance. The aims of this study were to MoreBackground and aim: Acinetobacter baumannii is an opportunistic pathogen that remains persistent in the environment for a long time. The eradication of this bacteria is difficult, since it has the ability to acquire antibiotic resistance. The aims of this study were to identify the ampC Betalactamas genes and antibiotic sensitivity of Acinetobacter baumannii strains.Materials and methods: Sixty three strains of Acinetobacter baumannii isolates from wound, blood, and respiratory secretions were isolated and identified by biochemical tests. Antimicrobial susceptibility testing against five antibiotics was performed by disc diffusion method for all isolates. Phenotypic methods combined disc test (CDT), was performed for idenfication AmpC Betalactamase activity in bacteria. The presence of AmpC Betalactamase and ISAba-1 and ISAba-2 genes were evaluated by PCR. Results: Disk difiusion result showed that all of samples were resistance to 5 antibiotics ceftriaxone, cefoxitin, ceftazidime, cefotaxime and cefepime. Combined Disk test result showed that (63%) of samples were strong (30%) weak and (6%) of negative. AmpC betalactamase gene in 90.5% were positive and in 9.5% were negative. All of sampls had ISAba-1 gene (69.2%) had ISAba-2. Conclusion: Current study showed a high percentage of AmpC Betalactamas genes and also high prevalence of antibiotic resistance in Acinetobacter baumannii Manuscript profile -
Open Access Article
4 - Evaluation of the effect of gold nanostructure on the production of bio surfactants from Pseudomonas aeruginosa
Yaser Ahsani Arani Zahra Noormohammadi behnam rasekh fatemeh Yazdian Hojjat kazemi Raminolipids belong to the group of glycolipid bio surfactants and were first isolated from Pseudomonas aeruginosa. Rhamnolipids are a good alternative to synthetic surfactants due to their low toxicity, biodegradability and selective performance. In this s More Raminolipids belong to the group of glycolipid bio surfactants and were first isolated from Pseudomonas aeruginosa. Rhamnolipids are a good alternative to synthetic surfactants due to their low toxicity, biodegradability and selective performance. In this study, the effect of gold (Au) nanoparticles on the growth and production of Pseudomonas aeruginosa PBCC5 bio surfactant was evaluated. Different concentrations of 1, 500 and 1000 mg / l nanoparticles were used. In this study, bio surfactant surface tension and emulsification indices (24E) were measured. The presence of nanoparticles on the bacterial surface was investigated by TEM and the morphology of nanoparticles was investigated by SEM. Binding of nanoparticles to bio surfactants was confirmed by TEM. The results showed that gold nanoparticles were not bactericidal and also increased bacterial growth and bio surfactant production. The surface tension of all samples was reduced from 72 mN / m distilled water to 35-32 mN / m. Manuscript profile -
Open Access Article
5 - Evaluation of the effect polysacharid nanocomposites on gtf gene expression in Streptococcus mutan
Shokofeh Mazhab jafari fatemeh Yazdian Farzaneh Hosseini behnam rasekhTooth decay is one of the most important public health problems in different communities. The activity of acid-producing bacteria, especially Streptococcus mutans biofilms, is the main cause of this complication. The formation of biofilms of this bacterium depends MoreTooth decay is one of the most important public health problems in different communities. The activity of acid-producing bacteria, especially Streptococcus mutans biofilms, is the main cause of this complication. The formation of biofilms of this bacterium depends on the presence of the enzyme glucosyltransferase, which is encoded by the gtf gene. The aim of this study was to evaluate the effect of polysaccharide nanocomposite of Chitosan (Cs)/Carboxymethyl Starch (CMS)/Graphene oxide (RGO)/Silica (SiO2) on gtf gene expression in Streptococcus mutans. Graphene oxide was synthesized by Hummers method. Chitosan/Carboxymethyl Starch/Graphene oxide/Silica quaternary compound were then prepared and antimicrobial effect was investigated and antimicrobial effect on Streptococcus's mutans ATCC 35668 was investigated by microdilution method. The morphology and structural properties were investigated by FTIR, SEM and DLS. The expression of gtfB, gtfC, gtfD genes was examined by RT-PCR technique. FTIR studies confirmed the incorporation of RGO and SiO2 into nanocomposite. The antimicrobial effect of nanocomposite (0.203 mg/ml) was obtained. RT-PCR results showed that no significant relationship was observed in the expression of gtfB and gtfD genes in cells treated with nanocomposites compared to the control group (P=0.187, P=0.067), while the expression of gtfC gene was significantly reduced (P=0.049) in cells treated with nanocomposite and indicating the strong effect of this nanocomposite on suppressing the gtfC gene and reducing biofilm formation. According to the results, the expression level of gftC gene in cells treated with nanocomposite was reduced, and Cs/CMS/RGO/SiO2 nanocomposite has a strong effect in suppressing gtfC gene. Manuscript profile -
Open Access Article
6 - Isolation and molecular identification of yeast cells and their evaluation for producing yeast extract and beta glucan
zohreh ranjbar majid baserisalehiYeast is a unicellular fungus with thick cell wall and nucleus. Nowadays this microorganism considered as important fungi in commercial industry because of their products specially yeast extract and beta glucan. The purpose of this study was isolation and identification MoreYeast is a unicellular fungus with thick cell wall and nucleus. Nowadays this microorganism considered as important fungi in commercial industry because of their products specially yeast extract and beta glucan. The purpose of this study was isolation and identification and screening of yeast cells for production of beta glucan and yeast cells. Yeast cells were isolated from plant and dairy samples and destruction of their cell walls was carried out using acidic condition, kiwi extract and change of osmotic pressure methods. In addition, isolation, purification and verification of beta glucan were done by modified Acid/Alkaline and Callose and Proton nuclear magnetic (H1NMR) methods respectively. The results obtained indicated that yeast cells were isolated from all samples. Two yeast cells based on their sizes and potential of growth were selected for further study. 18SrDNA gene sequencing of isolated strains identified them as Kluyveromyces marxianus and Saccharomyces cerevisiae. Our finding indicated that kiwi extract and change of osmotic pressure were favorable methods (p≤0.05) for production of yeast extract. Furthermore the results of Callose and H1NMR methods verified the production and purification of beta glucan. Hence based on our results production of beta glucan in industry scales is possible in our country Iran. Manuscript profile
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