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List of Articles
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Open Access Article
1 - Evaluation of antibody titers from vaccination against influenza virus subtype H9N2 in Japanese quails using dual Newcastle-Influenza vaccine
Golara Mardani Mehdi Rezaei Saman MahdaviAvian Influenza (AI) is one of the most destructive viral diseases of poultry flocks, and vaccination can be one of the most important ways to control the disease. The aim of this research was to evaluate the antibody titer obtained from vaccination against H9N2 influen MoreAvian Influenza (AI) is one of the most destructive viral diseases of poultry flocks, and vaccination can be one of the most important ways to control the disease. The aim of this research was to evaluate the antibody titer obtained from vaccination against H9N2 influenza virus in Japanese quails using Newcastle-influenza dual vaccine. For this purpose, 108 one-day-old Japanese quails were tested in 3 groups with 3 replicates. From day one to the end of the rearing period, the conditions were the same for all quails and the only difference was in the influenza disease vaccination program. The first group received killed ND/AI vaccine (CEVAC-NEW FLU H9 K) on day 7, the second group received the same type of vaccine on day 10 and the third group received no vaccination as the control group. The HI (Haemagglutination Inhibition) test was performed after two separate blood collection from the wing vein on days 25 and 35, following vaccination. Statistical analysis by Tukey's test showed that the mean titer of anti-influenza virus antibody in the serum of vaccinated birds was significantly different from the control group (p<0.05), so that in these groups, its amount was higher than its amount in the serum of birds of the control group. Also, the mean antibody titer in the serum of birds that received the vaccine at 7 days old was higher. Based on the findings of the present research and the low age of slaughter in broiler quail flocks, it seems necessary to carry out rapid vaccination against the influenza disease agent in the first week of rearing, to achieve the desired level of protective antibodies. Manuscript profile -
Open Access Article
2 - Cloning of Mycobacterium avium paratuberculosis hsp70 gene into pET-24a plasmid and its expression in Escherichia coli
Rasa Sheini mehrabzade Masoudreza Siefi abad shapori Masoud Ghorbanpoor Darush GharibiJohne's disease (Paratuberculosis) is a disease caused by Mycobacterium avium paratuberculosis (MAP) which is a chronic and progressive infection that chiefly affects ruminants. The disease can lead to significant economic losses in the livestock industry and may also b MoreJohne's disease (Paratuberculosis) is a disease caused by Mycobacterium avium paratuberculosis (MAP) which is a chronic and progressive infection that chiefly affects ruminants. The disease can lead to significant economic losses in the livestock industry and may also be a threat for public health; because it may be transmitted to humans through consumption of milk and other contaminated animal products. Immunity and resistance against the Johne's disease is mainly due to cell mediated immune response. Heat Shock Protein 70 (HSP70) of this bacterium is one of its important proteins, which the immune response against it can prevent the fecal excretion of bacteria. In order to facilitate the production of recombinant vaccine against Johne's disease, in this study the recombinant HSP (recombinant HSP; rHSP) was produced and its immunogenicity investigated in rabbits. For this purpose, the hsp70 gene was cloned into pET-24a plasmid and the resulting recombinant plasmid was transferred to E. coli strain BL21. The expression of the above protein was checked by SDS-PAGE and the accuracy of the nucleotide sequence was confirmed by sequencing. Immunization of rabbits by rHSP70 resulted in the production of high levels of antibodies. Based on the findings, it seems that the HSP70 specific antibody can be evaluated in the design of diagnostic methods of the disease and the produced recombinant protein can be assessed for the production of recombinant vaccines. Manuscript profile -
Open Access Article
3 - Isolation and identification of Helicobacter pullorum from cecal content and liver of broiler poultry referred to veterinary clinics in Tabriz using culture and PCR methods
Seyed mohammad Alavi shoushtari Afshin Javadi Golamreza Zarrini Hamid Mirzaei Shahram HanifianThe potential role of Helicobacter pullorum as an emerging cause of foodborne infection and a common disease between humans and livestock has been proven. This bacterium has been isolated from the contents of the cecum, liver and duodenum of laying and broiler poultry, MoreThe potential role of Helicobacter pullorum as an emerging cause of foodborne infection and a common disease between humans and livestock has been proven. This bacterium has been isolated from the contents of the cecum, liver and duodenum of laying and broiler poultry, as well as from the contents of the digestive system and bile ducts in human cases of gastro-intestinal inflammatory diseases. The aim of this study was to investigate Helicobacter pullorum infection in broiler poultry with symptoms of enteritis referred to one of the poultry clinics in Tabriz city. For this purpose, 70 samples of cecal contents and 70 liver samples from 14 broiler flocks were evaluated using bacterial culture and PCR (Polymerase Chain Reaction) methods. Based on the findings, 10 cecal content samples from 2 different herds were positive for the presence of Helicobacter pullorum bacteria in culture and molecular PCR tests, while none of the liver samples were positive in this respect. Considering the role of Helicobacter pullorum in causing mortality and damages in poultry farms and its related economic importance and the possibility of causing secondary carcass contamination during the slaughter due to presence of the bacterium in the digestive system of poultry as well as the zoonotic nature of the disease, therefore the above bacterium should always be considered as a health risk factor threatening the health of consumers. Manuscript profile -
Open Access Article
4 - Effect of ginseng hydro-alcoholic extract along with aerobic exercise on antioxidant levels and inflammatory factors of cardiac tissue in male rat model of endocarditis caused by Listeria Monocytogenes (ATCC: 19114)
Seyed Zanyar Athari Younes Anzabi Zahra Karamouz Mir Alireza NourazarEndocarditis is a rare but serious infection following listeriosis. Ginseng extract has immunomodulatory effects. Regular exercise reduces the risk of chronic metabolic and cardiopulmonary diseases through its anti-inflammatory effects. This study aimed to evaluate the MoreEndocarditis is a rare but serious infection following listeriosis. Ginseng extract has immunomodulatory effects. Regular exercise reduces the risk of chronic metabolic and cardiopulmonary diseases through its anti-inflammatory effects. This study aimed to evaluate the effect of ginseng extract against experimental endocarditis with Listeria monocytogenes in rats and the effect of aerobic exercise on improving its antimicrobial properties. For this purpose, 36 rats weighing 250±20 gr were randomly divided into six groups of 6, including sham, patient (receiving bacterial suspension), ampicillin treatment (15 mg/kg, s.c. for one month), ginseng treatment (0.025 mg/kg, i.p. for one month), aerobic exercise (60% of maximal oxygen consumption for one month) and ginseng+exercise (treated similarly to ginseng treatment and aerobic exercise groups). At the end of the study and after sacrifice, the levels of Total Antioxidant Capacity (TAC), Superoxide dismutase (SOD), Glutathione Peroxidase (GPx), and Paraoxonase-1 (PON-1), as well as the concentration of cardiac tissue cytokines including Interleukine-1 (IL-1), IL-6, IL-8, and TNF-α, were measured. Data were analyzed by ANOVA and Tukey post hoc test, and the significance level was considered as p<0.05. Tissue levels of antioxidants were significantly reduced in the patient group, while their levels were significantly increased in the ginseng+exercise group (p<0.05). On the other hand, tissue levels of IL-1, IL-6, IL-8, and TNF-α increased in the patient group, while their levels decreased significantly in the ginseng+exercise group (p<0.05). This study showed that hydro-alcoholic extract of ginseng along with aerobic exercise has a better effect than either of them alone against experimental endocarditis caused by listeria monocytogenesis. Manuscript profile -
Open Access Article
5 - Relationship of soil and forage Cobalt, Iron, Manganese, and Zinc contents with serum Cobalt, Zinc, vitamin B12 values and some hematological parameters in sheep of Ramhormoz city
Ali Abbas Nikvand Fatemeh Rastmanesh Mohammad Razi Jalali Elham GholamiIn ruminants, cobalt is essential for synthesis of vitamin B12 by rumen microflora. Emaciation and anemia are the most important consequences of cobalt deficiency in ruminants. This study aimed to investigate the relationship between cobalt, iron, zinc, and manganese le MoreIn ruminants, cobalt is essential for synthesis of vitamin B12 by rumen microflora. Emaciation and anemia are the most important consequences of cobalt deficiency in ruminants. This study aimed to investigate the relationship between cobalt, iron, zinc, and manganese levels of soil and forage with each other and with serum levels of cobalt, zinc, and vitamin B12 in emaciated and normal sheep of Ramhormoz city. Eight soil and 8 forage samples were taken from four areas in Ramhormoz city. The soil and forage samples were analyzed. Blood samples were collected from 25 (15 emaciated and 10 normal) and 43 sheep (28 emaciated and 15 normal) from areas with more and less than 0.07 μg cobalt/Kg of DM forage, respectively and were analyzed to determine serum cobalt, zinc, vitamin B12 values and some hematological parameters. The forage cobalt value in Plim (0.052±0.02 mg/kg dry matter (DM)) and Larkiabad (0.054±0.00 mg/kg DM) regions was lower than the standard value. The mean soil iron value (15767±1333 mg/kg arid soil) was more than its critical level in Ramhormoz. The highest soil manganese values in Larkiabad and Plim regions were associated with the lowest forage cobalt values. There was no statistically significant difference between serum levels of cobalt, zinc, and vitamin B12 in the emaciated and normal sheep. It is possible that the high amounts of soil iron and manganese, due to their chelating effects, could reduce the cobalt bio-availability of forage. Contrary to the available literature, the presence of 0.05 μg cobalt/Kg of DM forage is capable of providing sufficient amounts of cobalt and vitamin B12 for sheep in the region. Manuscript profile -
Open Access Article
6 - A comparative study of the frequency and intensity of nosemosis based on individual and composite samples of live bees in the apiaries of the cities of East Azerbaijan province
Abbas Imani Baran Gholamreza HamidianDue to the lack of an international standard method to determine the frequency, prevalence and intensity of nosemosis, the researchers are still using individual or composite sampling methods. This study was aimed to compare the frequency and intensity of nosemosis base MoreDue to the lack of an international standard method to determine the frequency, prevalence and intensity of nosemosis, the researchers are still using individual or composite sampling methods. This study was aimed to compare the frequency and intensity of nosemosis based on the above mentioned methods in the apiaries of East Azerbaijan province. To this end, 5 apiaries from 5 cities of East Azerbaijan province were randomly selected. Three hives from each apiary and 10 bees from each hive (equally from inside and outside) were sampled. To determine the frequency, the spore presence in the samples was evaluated. To determine the intensity, the spore count in one bee was calculated in the individual samples and mean spore count per bee in the composite samples. In both methods, 100% frequency was obtained for all samples. To compare infection intensity in both sampling methods, one-way analysis of variance and Tukey's post hoc test were used. To compare the results of two methods, generally, and also in each city, separately, independent T-test was used. In both methods, the highest intensity was related to samples from Tabriz (p<0.05). However, in the individual sampling method, the infection intensity in Osku samples was significantly lower (p<0.001). Also, in comparison of the two sampling methods, there was generally no statistical difference between the sampling methods and even in one city (p>0.05). In individual samples, overall, the intensity was higher in bees outside of the hive. Based on the average results, the intensity of nosemosis in composite samples was much higher than individual samples. Manuscript profile -
Open Access Article
7 - Antibacterial effects of cadmium sulfide nanoparticles producing by two methods of chemical precipitation and microwave on Escherichia coli isolates from poultry
Zahra Mohamdi Golafshan Jalal Shayegh Shahin TofangdarzadehPoultry colibacillosis causes several diseases that can cause great economic damage to poultry herds. Escherichia coli (E. coli) is a prominent member of this family, is known as one of the bacteria that pollutes the environment. Today, antibiotics and disinfectants are MorePoultry colibacillosis causes several diseases that can cause great economic damage to poultry herds. Escherichia coli (E. coli) is a prominent member of this family, is known as one of the bacteria that pollutes the environment. Today, antibiotics and disinfectants are used to prevent a variety of diseases. However, due to inappropriate consumption, as well as incomplete duration of treatment, antibiotic-resistant bacteria have emerged. Due to their small size and high surface-to-volume ratio, nanoparticles have particle inhibitory properties and therefore have many cell-killing effects that can be used as antibacterial, fungal and viral agents. In this study, the antibacterial effects of cadmium sulfide nanoparticles were investigated by chemical and microwave precipitation methods in Escherichia coli bacteria isolated from poultry. For this purpose, Escherichia coli bacterial samples were collected from poultry clinics in Tabriz in May and June 2016 .Synthesized cadmium sulfide nanoparticles and Identified by XRD, UV and SEM analysis were exposed to cultured E. coli by both precipitation and microwave methods. Results were determined based on the minimum amount of MBC bactericidal and the minimum inhibitory concentration of MIC. The MIC was 1.653% and the MBC was 2.051%, the MIC was 2.051% and the MBC was 1.653%. The results of this study showed that cadmium sulfide nanoparticles have good antimicrobial effects on Escherichia coli; however, no significant difference was observed between the synthesis method of these nanoparticles for bactericidal and bacteriostatic effects. Manuscript profile
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