Evaluation of antibody titers from vaccination against influenza virus subtype H9N2 in Japanese quails using dual Newcastle-Influenza vaccine
Subject Areas :
Veterinary Clinical Pathology
Golara Mardani
1
,
Mehdi Rezaei
2
,
Saman Mahdavi
3
1 - D.V.M Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia Branch,
Islamic Azad University, Urmia, Iran.
2 - Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
3 - Assistant Professor, Department of Microbiology, Maraghe Branch, Islamic Azad University, Maraghe, Iran.
Received: 2022-07-21
Accepted : 2022-11-15
Published : 2022-11-22
Keywords:
antibody titer,
Japanese quail,
Influenza virus,
Dual newcastle-influenza vaccine,
Abstract :
Avian Influenza (AI) is one of the most destructive viral diseases of poultry flocks, and vaccination can be one of the most important ways to control the disease. The aim of this research was to evaluate the antibody titer obtained from vaccination against H9N2 influenza virus in Japanese quails using Newcastle-influenza dual vaccine. For this purpose, 108 one-day-old Japanese quails were tested in 3 groups with 3 replicates. From day one to the end of the rearing period, the conditions were the same for all quails and the only difference was in the influenza disease vaccination program. The first group received killed ND/AI vaccine (CEVAC-NEW FLU H9 K) on day 7, the second group received the same type of vaccine on day 10 and the third group received no vaccination as the control group. The HI (Haemagglutination Inhibition) test was performed after two separate blood collection from the wing vein on days 25 and 35, following vaccination. Statistical analysis by Tukey's test showed that the mean titer of anti-influenza virus antibody in the serum of vaccinated birds was significantly different from the control group (p<0.05), so that in these groups, its amount was higher than its amount in the serum of birds of the control group. Also, the mean antibody titer in the serum of birds that received the vaccine at 7 days old was higher. Based on the findings of the present research and the low age of slaughter in broiler quail flocks, it seems necessary to carry out rapid vaccination against the influenza disease agent in the first week of rearing, to achieve the desired level of protective antibodies.
References:
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Azizpour, A., Bokaei, S., Sheikhi, N. and Habibzadeh S. (2012). A serological study of antibodies to H9N2 Avain influenza virus in human population of ardabil area, iran. Journal of Comprative Pathobiology, 9(1-36): 619-628. [In Persian]
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Pearson, J.E. (2003). International standards for the control of avian influenza. Avian Disease, 47(3 Suppl): 972-975.
Shariatmadari, F. (2000). Poultry production and the industry in Iran. World's Poultry Science Journal, 56(1): 55-65.
Suarez, D. and Schultz-Cherry, S. (2000). Immunology of avian influenza virus: a review. Developmental Comparative Immunology, 24(2-3): 269-283.
Swayne, D.E., Suarez, D.L. and Sims, D.L. (2013). Diseases of Poultry. 13th ed., USA: Iowa 50010, WILLY-BLACKWELL, pp: 181-218.
Talebi, E., Abedi, A., Rahimi, E. and Khosravinezhad, M. (2017). The effect of nano-selenium particles and sodium selenite on humoral immunity indices of quails using foods contaminated with aflatoxin B1. Veterinary Clinical Pathology, 11(42): 159-173. [In Persian]
Terregino, C., Toffan, A., Cilloni, F., Monne, I., Bertoli, E., Castellanos, L., et al. (2010). Evaluation of the protection induced by avian influenza vaccines containing a 1994 Mexican H5N2 LPAI seed strain against a 2008 Egyptian H5N1 HPAI virus belonging to clade 2.2.1 by means of serological and in vivo tests. Avian Pathology, 39(3): 215-222.
Wang, Y., Chen, X. and Yuan, Y. (2021). Antibody Response of an H9 Subtype Avian Influenza Poultry Vaccine on Three Kinds of Wild Birds in Shanghai Zoo. Avian Disease, 65(1): 90-94.
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Abolnik, C. (2014). A current review of avian influenza in pigeons and doves (Columbidae). Veterinary Microbiology, 170(3-4): 181-196.
Azizpour, A., Bokaei, S., Sheikhi, N. and Habibzadeh S. (2012). A serological study of antibodies to H9N2 Avain influenza virus in human population of ardabil area, iran. Journal of Comprative Pathobiology, 9(1-36): 619-628. [In Persian]
Alexander, D.J. (2008). Poultry Diseases. 6th ed., USA: Philadelphia, Elsevier Health Sciences press, pp: 317-332.
Breytenbach, J.H. (2005). Avian influenza control – the merits of vaccination. International Poultry Production, 13(4): 15-17.
Burgos, S. and Burgos. S.A. (2007). Influenza A Viruses in Poultry: A Condensed Review. International Journal of Poultry, 6(10): 705-708.
Iqbal, M., Yaqub, T., Reddy, K. and McCauley, J.W. (2009). Novel genotypes of H9N2 influenza a viruses, isolated from poultry in Pakistan containing NS genes similar to highly pathogenic H7N3 and H5N1 viruses. PloS one, 4(6): e5788.
Khan, S.U., Anderson, B.D., Heil, G.L., Liang, S. and Gray, G.C. (2015). A systematic review and meta-analysis of the seroprevalence of influenza A (H9N2) infection among humans. Journal of Infectious Disease, 212(4): 562-569.
Knipe, D.M. and Howley, P.M. (2013). Fields Virology. 6th ed., USA: Philadelfia, Lippincott Williams & Wilkins, pp: 1151-1185.
Mahdavi, S., Hemmatzadeh, F., Ghiami Rad, M. and Momtaz, M. (2017). Experimental Methods in Virology. 1st ed., Maragheh, Iran: Islamic Azad University of Maragheh Publication, pp: 70-77. [In Persian]
Mehrabanpour, M. (2019). Evaluation and comparison of the potential immunogenicity of two commercial inactivated bivalent Newcastle and avian Influenza vaccines in SPF chiken. Archives of Razi Institute, 74(3): 251-257.
Moradi, K. and Shahbazi, H.R. (2021). The effect of different levels of dietary threonine on performance, carcass characteristics, immune system and blood factors of Japanese quail under heat stress. Veterinary Clinical Pathology, 14(56): 365-379. [In Persian]
Myers, T.J. and Morgan, A.P. (2003). Policy and guidance for licensure of avian influenza vaccines in the United States. Avian Diseases, 47(special issue): 373-378.
Nili, H. and Asasi, K. (2002). Natural cases and an experimental study of H9N2 avian influenza in commercial broiler chickens of Iran. Avian Pathology, 31(3): 247-252.
(2015). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2016. 8th ed., Chapter 2.3.4. Avian Influenza (Infection with Avian Influenza Viruses). World Organization for Animal Health, Paris, France: Office International Des Epizooties, pp: 1594-1607.
Omidi zadeh, M., Kheyri, F. and Faghani, F. (2021). The effect of levels of coenzyme Q10 on performance, carcass characteristic, some blood parameters, immune system, organoleptic properties of meat and gastrointestinal tract development of male Japanese quails (Coturnix japonica). Journal of Animal Production, 23(1): 143-153. [In Persian]
Pearson, J.E. (2003). International standards for the control of avian influenza. Avian Disease, 47(3 Suppl): 972-975.
Shariatmadari, F. (2000). Poultry production and the industry in Iran. World's Poultry Science Journal, 56(1): 55-65.
Suarez, D. and Schultz-Cherry, S. (2000). Immunology of avian influenza virus: a review. Developmental Comparative Immunology, 24(2-3): 269-283.
Swayne, D.E., Suarez, D.L. and Sims, D.L. (2013). Diseases of Poultry. 13th ed., USA: Iowa 50010, WILLY-BLACKWELL, pp: 181-218.
Talebi, E., Abedi, A., Rahimi, E. and Khosravinezhad, M. (2017). The effect of nano-selenium particles and sodium selenite on humoral immunity indices of quails using foods contaminated with aflatoxin B1. Veterinary Clinical Pathology, 11(42): 159-173. [In Persian]
Terregino, C., Toffan, A., Cilloni, F., Monne, I., Bertoli, E., Castellanos, L., et al. (2010). Evaluation of the protection induced by avian influenza vaccines containing a 1994 Mexican H5N2 LPAI seed strain against a 2008 Egyptian H5N1 HPAI virus belonging to clade 2.2.1 by means of serological and in vivo tests. Avian Pathology, 39(3): 215-222.
Wang, Y., Chen, X. and Yuan, Y. (2021). Antibody Response of an H9 Subtype Avian Influenza Poultry Vaccine on Three Kinds of Wild Birds in Shanghai Zoo. Avian Disease, 65(1): 90-94.
Zhao, J., Yang, H., Xu, H., Ma, Z. and Zhang, G. (2017). Efficacy of an inactivated bivalent vaccine against the prevalent strains of Newcastle disease and H9N2 avian Influenza. Virology Journal, 14(1): 56.
