• Home
  • synthetic seed
    • List of Articles synthetic seed

      • Open Access Article

        1 - Plant regeneration of Citrus sinensis var. Thamson navel using sodium alginate-encapsulated shoot tips.
        Alam Ara Gholami Seyed Vahid Alavi
      • Open Access Article

        2 - Effective Use of Synthetic Seed Technology in the Regeneration of Dendrobium White Fairy Orchid
        Wee Leng Siew Mei Yun Kwok Yong Mei Ong Hui Ping Liew Boon Keat Yew
        The synthetic seed technology is becoming popular due to its wide application in germplasm conservation and for exchanges between countries in the floriculture trade. In this study, this method was used to study the germination and conversion capabilities of orchid spec More
        The synthetic seed technology is becoming popular due to its wide application in germplasm conservation and for exchanges between countries in the floriculture trade. In this study, this method was used to study the germination and conversion capabilities of orchid species Dendrobium White Fairy when stored at different storage times and with different storage containers. A high germination percentage of 80% was observed for encapsulated synthetic seeds up to 150 days of storage in Petri dishes and screw capped polypropylene tubes and this percentage only began to decline gradually from 150 storage days and more. Besides that, synthetic seeds stored in polypropylene tubes were shown to germinate faster and develop into plantlets with longer shoots and roots as compared to those stored in the Petri dishes. This proved the efficiency and suitability of polypropylene tubes over Petri dishes as storage containers. Manuscript profile
      • Open Access Article

        3 - Encapsulation of Protocorm of Cymbidium bicolor Lindl. for Short-Term Storage and Germplasm Exchange
        Ganesan Mahendran
        The present study describes the encapsulation of protocorm of Cymbidium bicolor Lindl. from 60 days-old in seed cultures for short- term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calc More
        The present study describes the encapsulation of protocorm of Cymbidium bicolor Lindl. from 60 days-old in seed cultures for short- term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were ob- tained through a combination of 3% sodium alginate and 100 mM calcium chloride. Encapsulated protocorms exhibited the best re-growth and con- version frequency on MS medium supplemented with BA (4.42 μM). En- capsulated protocorms stored at 25°C were green and retained the viability with potential for conversion (52%) and germination even after 360 days. The encapsulated protocorms stored at 4°C remained viable up to 30 days beyond which the conversion rate decreased drastically. Well-de veloped plantlets were transplanted into plastic pots containing vermiculite and maintained for 60 days in the culture room for acclimatization. The 90% of the recovered plantlets were hardened off and established suc-cessfully in the soil. The present study could be useful for large scale propagation as well as short term storage of this commercial orchid Manuscript profile