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        1 - Survey on prevalence of gastrointestinal parasites in wild goats (Capra aegagrus) of Arasbaran National Park
        Ahmad Nematoallahi Parisa Shahbazi Hadi Mardomi Amir Mollazadeh
        Arasbaran National Park is the largest national park in the west of the country, which is the habitat of important animal and plant species. One of the most important animal species living in it is the wild goat (Capra aegagrus). The aim of this study was to determine t More
        Arasbaran National Park is the largest national park in the west of the country, which is the habitat of important animal and plant species. One of the most important animal species living in it is the wild goat (Capra aegagrus). The aim of this study was to determine the level of parasitic infections of the gastrointestinal tract goats of Arasbaran National Park. During 2018, 95 fecal samples were collected from the wild goats of Arasbaran National Park and were examined by direct smear and Clyton-Lane methods. Bearman apparatus was also used to identify the first stage larvae of pulmonary nematodes. Iodine and modified Nelson staining were used to diagnose Cryptosporidium and Giardia infections, respectively. All samples showed at least one worm agent and one protozoan infection. In this study, Eimeria (spp.) infection was observed in all fecal samples and infection with egg of Marshallagia marshalli (37.8%), Nematodirus (spp.) (60%), Trichioris ovis (62.1%), Dicrocoelium dendriticum (8/37%), Fasciola hepatica (25.2%), Moniezia expansa (13.6%), Cystocaulus oecratus larvae (28.4%) and Giardia lambelia cyst (33.6%) were observed. Cryptosporidium oocysts were not observed in any of the stained stool samples. No significant relationship was found between infestation rate with different parasites and the sex of the infected animals. The high level of infection of parasites in wild goats of Arasbaran National Park indicates the high potential of parasitic infection and conversion of animals in it as a reservoir of parasites and its subsequent spread to other animals and expansion in neighboring human communities. Manuscript profile
      • Open Access Article

        2 - تأثیر مکمل تریپتوفان در جیره‌های با کمبود پروتئین بر عملکرد، توسعه دستگاه گوارش و پاسخ‌های ایمنی در جوجه‌های گوشتی
        م. فاطمی م. طغیانی
        تحقیق حاضر به منظور بررسی تأثیر مکمل کردن تریپتوفان در جیره‌های با کمبود پروتئین خام بر عملکرد، توسعه دستگاه گوارش و پاسخ‌های ایمنی در جوجه‌های گوشتی انجام شد. بدین منظور تعداد 420 قطعه جوجه گوشتی یکروزه (راس 308) در میان 6 تیمار با 5 تکرار و در قالب طرح کاملاً تصادفی ت More
        تحقیق حاضر به منظور بررسی تأثیر مکمل کردن تریپتوفان در جیره‌های با کمبود پروتئین خام بر عملکرد، توسعه دستگاه گوارش و پاسخ‌های ایمنی در جوجه‌های گوشتی انجام شد. بدین منظور تعداد 420 قطعه جوجه گوشتی یکروزه (راس 308) در میان 6 تیمار با 5 تکرار و در قالب طرح کاملاً تصادفی توزیع شدند. تیمارهای آزمایشی عبارتند از: 1) جیره شاهد (CTL) (جیره بر اساس نیاز توصیه شده کاتالوگ راس 2014 تنظیم شد)، 2) جیره شاهد + 15/0 درصد تریپتوفان، 3) جیره با کاهش سطح پروتئین خام 1 (LCP1) (10 درصد پروتئین کمتر از نیاز توصیه شده راس 2014)، 4) LCP1 + 15/0 درصد تریپتوفان، 5) جیره با کاهش سطح پروتئین خام 2 (LCP2) (20 درصد پروتئین کمتر از نیاز توصیه شده راس 2014) و 6) LCP2 + 15/0 درصد تریپتوفان. وزن بدن، مصرف خوراک روزانه و ضریب تبدیل جوجه‌ها در دوره‌های مختلف آزمایش مورد ارزیابی قرار گرفت. اندام‌های هضمی در روز 28 و انتهای آزمایش مورد ارزیابی قرار گرفتند. ریخت‌شناسی روده کوچک در روز 28 آزمایش بررسی شد. تست ترس در روز پایانی آزمایش انجام گرفت. جوجه‌های دریافت‌کننده جیره‌های با کاهش سطح پروتئین خام، وزن بدن و افزایش وزن روزانه پایین‌تری در انتهای دوره آغازین داشتند (05/0>P). مکمل نمودن تریپتوفان در جیره‌های LCP1 کاهش عملکرد رشد آنها را جبران نمود (05/0>P) اما تأثیری پس از افزودن به جیره‌های LCP2 نداشت. چربی محوطه شکمی در جوجه‌های دریافت‌کننده جیره‌های + Trp تمایل به کاهش نشان داد. جوجه‌های دریافت‌کننده جیره LCP2 در ژوژنوم و ایلئوم عمق کریپت بیشتر و نسبت طول پرز به عمق کریپت کمتری در مقایسه با گروه شاهد داشتند (05/0>P). تغذیه جوجه‌ها با جیره‌های LCP2 وLCP2  + 15/0 درصد تریپتوفان نسبت هتروفیل به لنفوسیت را در جوجه‌های گوشتی افزایش داد در حالیکه تأثیری بر طول زمان تست ترس نداشت. به طور کلی، مکمل نمودن تریپتوفان در جیره‌های LCP1 کاهش رشد جوجه‌های گوشتی را در مقایسه با جوجه‌های تغذیه شده با جیره‌های کم پروتئین کاهش داد. Manuscript profile
      • Open Access Article

        3 - Isolation and identification of microflora of farm ponds and gastrointestinal tract of western white shrimp (Litopenaeus vannamei) beside their evaluation as probiotic
        Mina Ziarati Mehran Avakh Keysami Farshid Kafilzadeh
        Background and Objectives: Nowadays, Shrimp culture has been prevailed in most of tropical countries; however, a variety of diseases have currently affected the business worldwide. Infectious bacterial agents are the most significant hygienic problems observed in the cu More
        Background and Objectives: Nowadays, Shrimp culture has been prevailed in most of tropical countries; however, a variety of diseases have currently affected the business worldwide. Infectious bacterial agents are the most significant hygienic problems observed in the cultured shrimps . This study was aimed to isolate and identify microflora of farm ponds and western white shrimp gastrointestinal tract (Litopenaeus vannamei) in the Bushehr Province farm ponds and their survey as probiotic. Materials and Methods: The shrimp and pond water samples were collected from 10 farm ponds (located at Bushehr Province). After transferring the samples to laboratory, the shrimp samples were autopsied to access to their intestinal microflora. The isolated bacteria were identified based on biochemical tests, morphology and Biolog software. Antibacterial activities of the isolates against 6 common pathogenic bacteria in shrimps were then investigated using well diffusion and disk diffusion methods. Results: Overall, 12 bacterial genera were isolated from the farm ponds and shrimp gastrointestinal tract. The majority of bacterial species most abundantly isolated were Bacillus, Vibrio and Pseudomonas. Among the isolated bacteria, Bacillus, Micrococcus and Corynebacterium showed the most antibacterial activity against evaluated pathogenic bacteria. Conclusion: Almost all the isolated bacteria from shrimp gastrointestinal tract were found in farm ponds as well. The bacterium Bacillus sp., for instance, is able to inhibit the growth of pathogens through secreting antimicrobial products. Manuscript profile
      • Open Access Article

        4 - Sand accumulation in the gastrointestinal tract and its relationship with its ultrasound evaluation of excretion stool in Kurd foal
        Rasoul Rahimzadeh
        Consistent intake of sand (and soil) with unprotected feed in the feces may cause it to accumulate in the gastrointestinal tract and lead to gastrointestinal disorders, This study is a report of the study of sand accumulation in the gastrointestinal tract of Kurdish hor More
        Consistent intake of sand (and soil) with unprotected feed in the feces may cause it to accumulate in the gastrointestinal tract and lead to gastrointestinal disorders, This study is a report of the study of sand accumulation in the gastrointestinal tract of Kurdish horses by diagnostic methods. So far, there have been no reports of horse butter from any breed in Iran. Therefore, none of the available studies includes the evaluation of the prevalence and diagnosis of sand without signs of accumulation in the gastrointestinal tract of foal. This study is also the first study to examine the foal. The aim of this study was to evaluate the level of sand accumulated in the gastrointestinal tract of healthy horses and to compare ultrasound and the amount of sand excreted. Abdominal ultrasound was evaluated. Ultrasound examination showed the presence of sand, sand was found in a single place, usually detected in the left diagnostic window. The mean amount of sand was 0.18 ± 0.21 g per 100 g of feces. Sand may accumulate in the gastrointestinal tract without any clinical signs. The amount of sand excreted in the stool does not indicate the amount of sand accumulated in the gastrointestinal tract. Abdominal ultrasound examination should be combined with it for more specific results. Manuscript profile
      • Open Access Article

        5 - Effects of Rice Hulls and Acidifier Supplementation on Growth Performance and Gut Physiology in Broiler Chickens
        S.  Banibugari M. Sedghi R.  Mirshekar A.H.  Mahdavi A.  Azarfar
        The experiment was conducted to study how adding rice hulls and an acidifier to the diet affected perform-ance, histomorphology, and microbial colonization in the gastrointestinal system of broiler chickens. A completely randomized design was used to test eight treatmen More
        The experiment was conducted to study how adding rice hulls and an acidifier to the diet affected perform-ance, histomorphology, and microbial colonization in the gastrointestinal system of broiler chickens. A completely randomized design was used to test eight treatments and five replications of 1000 day-old-male Arbor Acres broiler chickens (45±1 g). Dietary treatments included control (no additives 1-42 days), acidi-fier (control+0.1% acidifier from 1-42 days), RH10 (3% rice hull from 1-10 days and control diet from 11-42 days), RH10 + acidifier (3% rice hulls from 1-10 days and control diet+0.1% acidifier from 11-42 days), RH24 (3% rice hulls from 1-24 days and control diet from 25-42 days), RH24 + acidifier (3% rice hulls from 1-24 days and control diet+0.1% acidifier from 25-42 days), RH42 (3% rice hulls from 1-42 days) and RH42 + acidifier (3% of the rice hulls+0.1% acidifier from 1-42 days). Feed conversion ratio significantly decreased (P<0.05) in the RH24 + acidifier group compared with RH10 and control groups after 24 and 42 days. The RH42 + acidifierbirds had lower mortality than RH24+acidifier birds (P<0.05). Also, at 42 days of age, feeding RH42 + acidifier diet increased villus height/crypt depth (P<0.01). Acidifiers increased the Lactobacillus population in the small intestine of broiler chickens. The lowest gizzard pH was observed in birds fed 3% rice hulls from 0 to 42 days of age (P<0.05). In general, the addition of 30 kg/ton of rice hulls to the broiler feed, from 0 to 42 days of age, caused the highest possible weight gain and the lowest mortal-ity. Manuscript profile
      • Open Access Article

        6 - The Effects of Dietary Inclusion of Arginine and Rice Hull on Growth Performance, Immune Responses, Intestinal Morphology, Tibia Bone Mineral Content and Intestinal Microbial Population of Broiler Chickens
        M.  Abbasabadi S.M.  Hosseini H.  Naeimipour F.  Izadi Yazdanabadi
        Arginine and fibers may have positive effects on gastrointestinal tract and indirectly affect immune re-sponses, growth performance, intestinal morphology and microbial population. This study was conducted to evaluate the effects of dietary inclusion of arginine and ric More
        Arginine and fibers may have positive effects on gastrointestinal tract and indirectly affect immune re-sponses, growth performance, intestinal morphology and microbial population. This study was conducted to evaluate the effects of dietary inclusion of arginine and rice hull on growth performance, immune re-sponses, intestinal morphology and intestinal microbial population of broiler chickens. Three hundred and sixty male broiler chickens were randomly allocated into nine dietary treatments with four replications of 10 broiler chickens based on a factorial arrangement (3×3) in a completely randomized design. Experimen-tal treatments were including basal diets supplemented with arginine (90, 100, and 110% recommended levels), and fiber (0.00, 2.50, and 5.00%). Growth performance, immune responses, intestinal morphology, tibia bone mineral content and intestinal microbial population of broiler chickens were measured. The ef-fects of dietary inclusion of rice hull, arginine, and their interactions were significant on average daily gain (P<0.05) and feed conversion ratio (FCR) (P<0.05), so that highest gain and the lowest feed conversion ratio were observed in broiler chickens fed with highest levels of arginine (110.00%) and fiber (5.00%). Dietary supplementation of arginine progressively increased immune responses (P<0.05), while inclusion of rice hull increased villus height and crypt depth (P<0.05) and decreased the concentrations of calcium (P<0.05), phosphorous (P<0.05) and magnesium (P<0.05). The inclusion of arginine (P<0.05), and fiber (P<0.05) in higher levels increased Lactobacilli population (P<0.05). In total, higher levels of fiber (5.00%) and arginine (110.00%) improved the growth performance and are suggested in diet of broiler chickens for improving growth performance. Manuscript profile
      • Open Access Article

        7 - The Effect of Various Dietary Fibre Sources on Performance, Cecal and Ileal Nutrient Digestibility in Broiler Chickens
        Y.  Mohammadi A.A. Saki S.  Mirzaei Goudarzi A.  Ahmadi Z. Bardel S.  Mohammadian
        This experiment was conducted to investigate the effects of different sources of insoluble fiber on perform-ance, gastrointestinal tract characteristics, and nutrient digestibility in the broiler's ileum, and cecum. A total of 380-day-old chickens of ross 308 unsexed we More
        This experiment was conducted to investigate the effects of different sources of insoluble fiber on perform-ance, gastrointestinal tract characteristics, and nutrient digestibility in the broiler's ileum, and cecum. A total of 380-day-old chickens of ross 308 unsexed were arranged into 5 treatments, 4 replicates, and 19 chickens in each, by completely randomized design (CRD). Experimental treatments included 1: control (corn-soybean meal) 2: arbocel (synthetic fiber) 1% in diet, 3: sunflower hulls (3% in diet), 4: soybean hull (3% in diet), 5: processed wheat straw (3%in diet). Observed parameter include: performance (feed intake, feed conversion ratio and body weight gain), gastrointestinal pH, gastrointestinal viscosity and digestibility of dry matter and protein in ileum and cecum and microbial population in ileum and cecum. The results have shown that the inclusion of 3% sunflower hulls in diets leads to the higher average feed intake from 1 to 10 days of age rather than control (P<0.05). In comparison between the ileum and cecum showed that the higher viscosity by Arbocel® in the cecum and the lowest viscosity showed by wheat straw in the ileum (P<0.05). On the other hand, the digestibility of protein and dry matter in the ileum was higher than the cecum (P<0.05). According to the results of this study, it could be noted that all fibers are considered in-soluble fibers and their different behavior in the gastrointestinal tract. The diet Arbocel® has generally led to physicochemical changes (digesta pH and viscosity) and microbial populations. It could be the most im-portant reason for these observations which is related to insoluble fiber particle size and their processing. In addition, reduced dry matter and protein digestibility in the cecum in comparison to the ileum status. Fi-nally, digest pH, viscosity, dry matter and protein digestibility were reduced by insoluble fiber in cecum in comparison to ileum. Manuscript profile