List of Articles Suspension culture

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  1 - Evaluation of the growth regulators, sugars and amino acid tryptophan effect on the rate of tryptophan decarboxylase enzyme on Vinca major L. in tissue culture, cell suspension culture and field culture conditions
  Ali Kazemzadeh fariba khosravinejad Amirhossein Kazemzadeh Haghighi
  10.30495/zisti.2023.1976340.1150
  This study was performed to investigate the effect of growth regulatory treatments on the activity of tryptophan decarboxylase (TDC) which is a cytosolic enzyme and a key enzyme in the secondary metabolites Pathway with two subunits,115-118 KDa, molecular mass and depen More

  This study was performed to investigate the effect of growth regulatory treatments on the activity of tryptophan decarboxylase (TDC) which is a cytosolic enzyme and a key enzyme in the secondary metabolites Pathway with two subunits,115-118 KDa, molecular mass and dependent on pyrodoxal phosphate (B6), Km75µmole for typtophan with optimal activity at alkaline pH of 7.5 in V.major hypocotyl. Some of simultaneous use growth regulators KIN, BA, NAA and IAA in three concentrations (0.1, 0,5, 1,0 mg/L) as well as various treatments of sucrose, fructose and glucose in 3 concentrations (2% , 4% , 6%) and also the amino acid tryptophan in concentrations (100 and 200 mg/L) were used to measure the amount of enzyme TDC activity, which was performed in three replications as a factorial in a completely randomized farm design of Karaj Seed and Plant Breeding Research Institute. In both of IAA and NAA at concentrations of 0.5 mg/L increases the amount of enzyme TDC in It has become about 10%. But in cytokines, KIN and BA at 1,0 mg/L had the greatest effect of about 40%. Sugars such as Sucrose in concentrations 6%, fructose and glucose in 4% and amino acid tryptophan in concentration 100 mg/l had the best effect on the activity of enzyme TDC in three replications as Factorial in the form of a completely randomized design in some cases. Manuscript profile
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  2 - The Effect of Plant Growth Regulators and Different Explants on the Response of Tissue Culture and Cell Suspension Cultures of German Chamomile (Matricaria chamomilla L.)
  L. Koohi, N. Zare R. Asghari-Zakaria P. SheikhZadeh-Mosaddegh
  German chamomile (Matricaria chamomilla L.) is one of the most important medicinal plants that its essential oils used in different medicinal industries. In this study which was carried out in 2013 growing season at the Faculty of Agricultural Sciences of the University More

  German chamomile (Matricaria chamomilla L.) is one of the most important medicinal plants that its essential oils used in different medicinal industries. In this study which was carried out in 2013 growing season at the Faculty of Agricultural Sciences of the University of Mohaghegh Ardabili, the in vitro response of leaf and hypocotyl explants of German Chamomile in B5 medium supplemented with different levels of plant growth regulators including 2,4-D, naphthalene acetic acid (NAA), kinetin and 6-benzylaminopurine (BAP) were investigated in a factorial experiment based on completely randomized design (CRD).In addition, cell suspension cultures were established and characterized. Hypocotyl and leaf explants exhibited cell proliferation and produced callus within 1-2 weeks. The highest fresh weight of the callus (264.1 mg) was produced by leaf explants in the medium supplemented with 0.5 mg/l 2,4-D and 1 mg/l BAP. However, the leaf explants cultured on medium containing 1.5 mg/l 2,4-D showed the lowest cell proliferation and callus yield (40.42 mg). The highest percentage of root induction from leaf explants (58.73%) was observed on the medium containing 4 mg/l 2,4-D and 1 mg/l Kin, and from hypocotyl explants (48.61%) was observed on medium supplemented with 1.5 mg/l NAA. The 42.22% of calli derived from hypocotyl explants on B5 medium supplemented with 4 mg/l NAA and 3 mg/l BAP, were friable. Cell suspension cultures of German chamomile were established by transferring of hypocotyl-derived friable calli into the MS medium supplemented with 1.5 mg/l 2,4-D and 1 mg/l kinetin. The growth curve of cell proliferations started 4 days after culture and continued to grow until day 13th, where the cells entered stationary phase. Manuscript profile
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  3 - Cell suspension culture of yew: an alternative method of taxol production
  raheleh gorzi
  10.30495/ijpp.2020.691457
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  4 - In Vitro Effect of Plant Growth Regulators on Callogenesis and Suspension Culture Optimization of Portulaca oleracea L.
  Manizheh Jamshidi
  10.30495/jdb.2021.680725
  In this research, the effect of type and concentration of BAP and 2,4-D plant growth regulators on callogenesis of hypocotyl and leaf explants of Portulaca oleracea in MS medium containing 0.5, 1 and 2 mg/L of 2,4-D and BAP and optimization of cell suspension culture wi More

  In this research, the effect of type and concentration of BAP and 2,4-D plant growth regulators on callogenesis of hypocotyl and leaf explants of Portulaca oleracea in MS medium containing 0.5, 1 and 2 mg/L of 2,4-D and BAP and optimization of cell suspension culture with different plant growth regulators in MS medium containing 0.5, 1 and 2 mg/L 2,4-D, 0.5 and 1 mg/L BAP, 0.2 and 0.5 mg/L Kin each one containing 20 and 30 g/l sucrose concentration, were investigated. Based on the results, both of the samples had 100 percentage callus induction, but the function of hypocotyl callus was significantly higher than the leaf callus. MS culture media containing 2 mg/l BAP and 0.5 mg/l 2,4-D, 2 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 0.5 mg/l 2,4-D, 0.5 mg/l BAP And 0.5 mg/l 2,4-D and 1 mg/l BAP and 2 mg/l 2,4-D, all containing 150 mg/l ascorbic acid in both explants have the highest yield (wet weight of callus). In the cell suspension culture, there was a significant difference between treatments with the plant growth regulators in terms of number of cells in per ml, SCV, PCV and treatment duration. Manuscript profile
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  5 - Evaluation of the growth regulators, sugars and amino acid tryptophan effect on the rate of tryptophan decarboxylase enzyme on Catharanthus roseus (L.) G.Don. in tissue culture, cell suspension culture and field culture conditions
  ali kazemzadeh haghighi hamid sobhanian gholamreza bakhshi khaniki mohamadali ebrahimi
  10.30495/jdb.2023.700350
  Catharanthus roseus The most important species from Apocyanaceae contains an important and special group of indole alkaloids with a great medicinal value, which are distributed in all organs of the plant. The presence of valuable alkaloids in all organs of the plant has More

  Catharanthus roseus The most important species from Apocyanaceae contains an important and special group of indole alkaloids with a great medicinal value, which are distributed in all organs of the plant. The presence of valuable alkaloids in all organs of the plant has led to the introduction of this species as very important medicinal plants and their properties to be studied. This study was performed to investigate the effect of growth regulatory treatments on the activity of tryptophan decarboxylase (TDC) which is a cytosolic enzyme and a key enzyme in the secondary metabolite biosynthesis The effects of some growth regulators such as KIN, BA, NAA and IAA in three concentrations (0.1, 0.5, 1.0 mg/L) as well as various sugar treatments like sucrose, fructose and glucose in 3 concentrations (2% , 4% , 6%) and also the amino acid tryptophan (at concentrations 100 and 200 mg/L, were investigated on TDC enzyme activity. The experimant was performed in three replications as a factorial in a completely randomized design in Karaj Seed and Plant Breeding Research Institute. in the applied IAA and NAA at concentrations of 0.1 and 0.5 mg/L respectively, increased the activity of TDC by about 10%. But cytokinins, KIN and BA at 1,0 mg/L had the greatest promotion effect of about 40%. Sugars such as Sucrose in concentrations 6%, fructose and glucose in 4% and amino acid tryptophan in concentration 100 mg/l had the maximal effects on the activity of TDC enzyme Manuscript profile
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  6 - Suspension culture of Neospora caninum using lymphocyte transformed by Theileria lestoquardi
  Shamsi Ezhdahakosh pour Mohammad Mehdi Namavari Nahid Naghshgar Abdollah Rahimian Maryam Mansoorian Masoume Hayati
  Background and Objectives: Neospora caninum is one of main etiologic factors of abortion in  in cattle. The mass production of protozoa in laboratory conditions is performed based on growing in different cell lines. Since using suspension cell lines are very cost e More

  Background and Objectives: Neospora caninum is one of main etiologic factors of abortion in  in cattle. The mass production of protozoa in laboratory conditions is performed based on growing in different cell lines. Since using suspension cell lines are very cost effective for mass production of biological products, this study aimed to evaluate the suspension cell culturing for production of this protozoan. Materials and Methods: This study was experimentally performed bases on growth of N. caninum tachyzoites on Ka6 cell line (a cell line obtained from cattle infected with Theileria lestoquardi, Razi Institute, Shiraz, Iran. Next, based on MTT assay, ability of this cell culture for production of  N. caninum was compared with  Vero cell as the best current cell line for this purpose. Results: The results showed that N. caninum tachyzoites could enter into K6a cell lines and after replication released from the cells successfully. Replication of the tachyzoites was significant in both Vero and K6a cell lines in compare to the control. Conclusion: To our knowledge, this is the first and successful report of suspension culture of N. caninum tachyzoites. Although, the rate of N. caninum proliferation on Ka6 cell line did not show any significant difference in compare to Vero cell line, since Ka6 cell line is a transformed lymphocytic cell and it is possible to grow massively this cell line as suspension bioreactors, it is preferred to Vero cell line. Manuscript profile