Evaluation of the growth regulators, sugars and amino acid tryptophan effect on the rate of tryptophan decarboxylase enzyme on Vinca major L. in tissue culture, cell suspension culture and field culture conditions

Subject Areas : plant physiology

Ali Kazemzadeh ¹ , fariba khosravinejad ² , Amirhossein Kazemzadeh Haghighi ³

1 - Department of biology, faculty of biological sciences, north-tehran branches, islamic azad university, tehran, iran
2 - department of biology, faculty of biological sciences, north-tehran branches, islamic azad university, tehran, iran.
3 - College of science, University of Tehran, Tehran, Iran

Received: 2022-12-30 Accepted : 2023-04-13 Published : 2022-11-22

Keywords: Cell suspension culture, Field culture, tryptophan decarboxylase, Vinca major,

Abstract :

This study was performed to investigate the effect of growth regulatory treatments on the activity of tryptophan decarboxylase (TDC) which is a cytosolic enzyme and a key enzyme in the secondary metabolites Pathway with two subunits,115-118 KDa, molecular mass and dependent on pyrodoxal phosphate (B6), Km75µmole for typtophan with optimal activity at alkaline pH of 7.5 in V.major hypocotyl. Some of simultaneous use growth regulators KIN, BA, NAA and IAA in three concentrations (0.1, 0,5, 1,0 mg/L) as well as various treatments of sucrose, fructose and glucose in 3 concentrations (2% , 4% , 6%) and also the amino acid tryptophan in concentrations (100 and 200 mg/L) were used to measure the amount of enzyme TDC activity, which was performed in three replications as a factorial in a completely randomized farm design of Karaj Seed and Plant Breeding Research Institute. In both of IAA and NAA at concentrations of 0.5 mg/L increases the amount of enzyme TDC in It has become about 10%. But in cytokines, KIN and BA at 1,0 mg/L had the greatest effect of about 40%. Sugars such as Sucrose in concentrations 6%, fructose and glucose in 4% and amino acid tryptophan in concentration 100 mg/l had the best effect on the activity of enzyme TDC in three replications as Factorial in the form of a completely randomized design in some cases.