In Vitro Effect of Plant Growth Regulators on Callogenesis and Suspension Culture Optimization of Portulaca oleracea L.

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Manizheh  Jamshidi ¹ (Plant protection Department Tabriz Branch)

Received: 2020-12-28 Accepted : 2021-02-15 Published : 2021-02-19

Keywords: callogenesis, Portulaca oleracea L, Suspension culture,

Abstract :

In this research, the effect of type and concentration of BAP and 2,4-D plant growth regulators on callogenesis of hypocotyl and leaf explants of Portulaca oleracea in MS medium containing 0.5, 1 and 2 mg/L of 2,4-D and BAP and optimization of cell suspension culture with different plant growth regulators in MS medium containing 0.5, 1 and 2 mg/L 2,4-D, 0.5 and 1 mg/L BAP, 0.2 and 0.5 mg/L Kin each one containing 20 and 30 g/l sucrose concentration, were investigated. Based on the results, both of the samples had 100 percentage callus induction, but the function of hypocotyl callus was significantly higher than the leaf callus. MS culture media containing 2 mg/l BAP and 0.5 mg/l 2,4-D, 2 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 0.5 mg/l 2,4-D, 0.5 mg/l BAP And 0.5 mg/l 2,4-D and 1 mg/l BAP and 2 mg/l 2,4-D, all containing 150 mg/l ascorbic acid in both explants have the highest yield (wet weight of callus). In the cell suspension culture, there was a significant difference between treatments with the plant growth regulators in terms of number of cells in per ml, SCV, PCV and treatment duration.