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      • Open Access Article

        1 - Reducing Arsenic Toxicity Stress in Soybean (Glycine max L.) by Using of Sodium Nitroprusside
        Elham Asadi karam Batool Keramat Hossein Mozaffari
        Arsenic contamination is one of the most important compounds all over the world. Arsenic in different ways, including the formation of reactive oxygen species and membrane lipid peroxidation impairs growth of plants. In this study, the effect of arsenic and sodium nitro More
        Arsenic contamination is one of the most important compounds all over the world. Arsenic in different ways, including the formation of reactive oxygen species and membrane lipid peroxidation impairs growth of plants. In this study, the effect of arsenic and sodium nitroprusside (SNP) were evaluated on soybean. Soybean at four leaf stage was treated with different concentrations of arsenic (0, 150 and 300 mM) and SNP (0 and 100 mM), and then concentrations of hydrogen peroxide, proline, malondialdehyde and activity of antioxidant enzymes in its shoot were measured. The results showed that increasing concentration of arsenic in Hoagland solution reduced total chlorophyll content in the shoot and increased the activity of catalase and peroxidase significantly. It was also observed that treating plants with arsenic increased hydrogen peroxid accumulation which resulted in peroxidation of membrane lipids. Higher malondialdehyde content confirmed this result. Using SNP in the medium containing arsenic increased total chlorophyll content, activities of guaiacol peroxidase enzyme and ascorbate peroxidase, significantly. However, the catalase activity in this case decreased. Thus it can be concluded that using SNP, would reduce effectively the damage of oxidation. Manuscript profile
      • Open Access Article

        2 - Investigation the role of nitric oxide on drought tolerance of mung bean (Vigna radiata L.)
        Omid Sadeghipour
        Drought is one of the most important adverse environmental factors that limits crop production. Nitric oxide (NO) as a signaling molecule contributes in plant response to environmental stresses. Therefore, in order to investigate the effects of NO on drought tolerance o More
        Drought is one of the most important adverse environmental factors that limits crop production. Nitric oxide (NO) as a signaling molecule contributes in plant response to environmental stresses. Therefore, in order to investigate the effects of NO on drought tolerance of mung bean (cv. Partow), a field experiment was done in the south of Tehran in 2015. The study was conducted as a randomized complete block design with 9 treatments and 4 replications. In this experiment, sodium nitroprusside (SNP) solution was used as NO donor. Treatments included control, drought stress, seed treatment, foliar application at the vegetative stage, foliar application at the reproductive stage, seed treatment + foliar application at the vegetative stage, seed treatment + foliar application at the reproductive stage, foliar application at the vegetative and reproductive stages, and seed treatment + foliar application at the vegetative and reproductive stages. Results showed that due to the drought stress, antioxidant enzymes activities such as superoxide dismutase, catalase, and ascorbate peroxidase, as well as malondialdehyde (MDA) and proline concentrations were increased but the relative water content (RWC), chlorophyll value, leaf area index (LAI), and seed yield decreased. However, application of SNP by further increasing the activity of antioxidant enzymes and proline accumulation, reduced the MDA content while improving the RWC, chlorophyll value, LAI, and eventually the seed yield under drought stress. Among SNP application treatments, seed treatment + foliar application at the vegetative and reproductive stages was the most effective, although it was not significantly difference from the other treatments including SNP foliar application at the reproductive stage. According to the findings of the study, application of NO may be recommended as a useful tool for improving drought tolerance of mung bean. Manuscript profile
      • Open Access Article

        3 - Effects of sodium nitroprusside and potassium silicate on the growth and flowering of Gazania rigens (L.)
        Zahra Nasiri Elham Danaee
        Gazania, belongs to the family Asteraceae, is widely grown in gardens and other environments. The purpose of this study was to evaluated the effects of sodium nitroprusside and potassium silicate treatments on the growth and flowering of gazania. For these purposes, a c More
        Gazania, belongs to the family Asteraceae, is widely grown in gardens and other environments. The purpose of this study was to evaluated the effects of sodium nitroprusside and potassium silicate treatments on the growth and flowering of gazania. For these purposes, a completely randomized design was used, with three levels of sodium nitroprusside (SNP) (25, 50 and 100 μM/l), potassium silicate (PS) at the concentration of 25, 50 and 100 mg/l and control. Some important traits such as fresh and dry weight of shoot and root, number of flowers, root volume, longest root length, plant height, cell membrane stability index, petiole carotenoid, leaf chlorophyll, protein, superoxide dismutase (SOD) and peroxidase (POD) enzyme activity and flower longevity. It was observed that plan that shoot fresh/dry weight, flower number, root length, plant height, carotenoid, leaf chlorophyll was increased under 100 mg/l PS treatment. The results suggest that the application of 50 μM/l SNP has favorable effects on the raise root fresh/dry weight, root volume, cell membrane stability index, protein, SOD and POD activity. Over the growth stage, 50 mg/l PS was found to be the best treatment to maintain flower longevity with 7.2 days. Manuscript profile
      • Open Access Article

        4 - Effects of the Timing of Foliar Application and Concentrations of Growth Regulators on the Mineral Content of Pistachio Leaves
        Hamid Alipour Mohammad Kafi Ahmad Nezami Amir Mohammadi
      • Open Access Article

        5 - Relationship of Sodium Nitroprusside with Growth and Antioxidant Enzymes of Canola under Lead Stress
        Hossein Hamidi Nahid Masoudian Mostafa Ebadi Bostan Roudi
      • Open Access Article

        6 - Study of sodium nitroprusside spraying on the agronomic and physiological traits of safflower (Carthamus tinctorius L.) in arsenic contaminated soils.
        meysam oveysi mansour faragi pourang kasraei
        In order to investigate the effect of sodium nitroprusside levels on growth and quantitative and qualitative characteristics of safflower (Carthamus tinctorius L.) in arsenic contaminated soils, a pot experiment was conducted in a research greenhouse of Faculty of Agric More
        In order to investigate the effect of sodium nitroprusside levels on growth and quantitative and qualitative characteristics of safflower (Carthamus tinctorius L.) in arsenic contaminated soils, a pot experiment was conducted in a research greenhouse of Faculty of Agriculture, Islamic Azad University, Varamin-Pishva Branch, in 2016-2017 in factorial experiment and in the form of design Completely randomized in 3 replications. The experimental treatments included of sodium nitroprusside spraying at three levels: (0, 50 and 100 μm) and soil contamination with arsenic at four levels (0, 30, 60 and 90 mg of arsenic per kg of soil). The results showed that arsenic and sodium nitroprusside had a significant effect on grain yield, 1000 grain weight, catalase enzyme, malondialdehyde content, proline content and safflower arsenic content. The highest grain yield was obtained in sodium nitroprusside treatments in application of 100 μM (with an average of 1.01 g / plant), and the lowest was related to control treatment (no application) with mean of 0.83 g / plant. By increasing the concentration of arsenic, yield and yield components decreased, and the plant increased its proline content to cope with stress. Application of sodium nitroproside reduced stress in plants under arsenic toxicity. No significant differences were observed between the concentrations of sodium nitroprusside spraying. With increasing arsenic levels, the amount of arsenic content increased and sodium nitroprusside reduced the amount of arsenic in the seed. In general, use of sodium nitroprusside as a regulator in the metabolism of free oxygen radicals reduces the effects of oxidative stress caused by soil contamination with arsenic.   Manuscript profile
      • Open Access Article

        7 - The effect of foliar application of brassinosteroid (BR) and sodium nitroprusside (SNP) on pigment content of Borago (Borago officinalis L.) under salinity stress conditions
        S.M Mahdaei رضا Monem A.R Pazoki
        In order to investigate the effect of Effect of brassinosteroids and sodium nitroprusside (SNP) foliar application on physiological, biochemical and morphological traits of Borago (Borago officinalis L.) under salt stress conditions, based on a greenhouse experiment was More
        In order to investigate the effect of Effect of brassinosteroids and sodium nitroprusside (SNP) foliar application on physiological, biochemical and morphological traits of Borago (Borago officinalis L.) under salt stress conditions, based on a greenhouse experiment was conducted in Shahriar region in 2016-2017. The experiment was conducted as factorial based on completely randomized design with four replications. In which salinity stress from the source of Nacl at three levels (0, 40 and 80 mM), Nitroprosium sodium sulfate was applied at three levels (0, 75 and 150 μM) and brassinosteroids at two levels (0 and 1.5 μm) Were considered. The simple effect of brassinosteroid and sodium nitroprusside on all experimented traits and the interaction effect of experimental factors on carotenoid and xanthophyll content were significant. In these conditions, after increase in salinity stress, the pigment content decreased and these traits improved with anti-stress compounds consumption. So the highest amount of carotenoid and xanthophyll with 1.6667 mg/g and 0.3344 mg/g were obtained with 150 μM sodium nitroprusside and 1.5 μM brassinosteroids consumption. Manuscript profile
      • Open Access Article

        8 - The effect of foliar application of sodium nitroprusside (SNP) and brassinosteroids (BNs) on root and shoot vegetative traits of Rosemary (Rosmarinus officinalis L.) under salinity stress conditions
        S.S Ghazaei
        Due to study the effect of foliar application of sodium nitroprusside (SNP) and brassinosteroids (BNs) on root and shoot vegetative traits of rosemary (Rosmarinus officinalis L.) under salinity stress conditions, a greenhouse experiment was done at shahr-e-rey region 20 More
        Due to study the effect of foliar application of sodium nitroprusside (SNP) and brassinosteroids (BNs) on root and shoot vegetative traits of rosemary (Rosmarinus officinalis L.) under salinity stress conditions, a greenhouse experiment was done at shahr-e-rey region 2016-2017. The experiment was conducted as factorial based on completely randomized design with four replications. In which salinity stress from the source of Nacl at three levels (0, 40 and 80 mM), Sodium nitroprusside at three levels (0, 75 and 150 μM) and Brassinosteroids at two levels (0 and 1.5 μm) were considered. The analysis of variance results showed that the simple effects of salinity stress, application of salicylic acid and jasmonic acid were significant on all investigated subjects, and among these traits, root length, number of leaves and root dry weight were influenced by the triple interaction of salinity stress, salicylic acid and jasmonic acid. The results of mean comparison showed that in 95 mM sodium chloride consumption the lowest plant height (41.93 cm), root length (9.79 cm), leaves number (4.62), leaf area of plant (140.73), shoot fresh weight (12.93 g/plant), shoot dry weight (2.46 g/plant) and root dry weight (1.42 g/plant) were observed. The results of mean comparison double interaction effects confirmed that maximum amount of shoot dry weight (11.8867 g/plant), root dry weight (3.7811 g/plant) and leaf area (675.63 cm2) were obtained at 150 μM of sodium nitroprusside and 1.5 μM of brassinosteroid. Therefore, it can be concluded that consumption of sodium nitroprusside and brassinosteroid improved all morphological traits tested in rosemary medicinal plant through the occurrence of anti-stress effects. Manuscript profile