• List of Articles Pyrus

      • Open Access Article

        1 - Comparative anatomical study of leaf and petiole in three species of pear (Pyrus L.) in Guilan Province
        Nasibeh Dehghan Siavash Hosseini Sarghein Rashid Jamei
        Objectives: The aim of this study was comparing the anatomical characters of leaves and petioles of 3 species of pear (Pyrus boissieriana Buhse, Pyrus communis L, Pyrus hyrcana Fedor) in Guilan Province. Materials and Methods: In this study, for anatomical study of spec More
        Objectives: The aim of this study was comparing the anatomical characters of leaves and petioles of 3 species of pear (Pyrus boissieriana Buhse, Pyrus communis L, Pyrus hyrcana Fedor) in Guilan Province. Materials and Methods: In this study, for anatomical study of species, at first, plant samples were collected. Then the manual section was performed in the laboratory. Carmine staining was used in order to stain the samples. After staining, slides were prepared from the cross-sectional area. For observing the transverse sections of leaves and petiole, optical microscope equipped with a scaled lens was used. Then, the data obtained from anatomical characters were statistically analyzed. Results: Leaf and petiole thickness were different among three tested species. The highest midrib thickness belonged to p < /em>. communis (682±1.11μm). The lowest midrib thickness was observed in p < /em>. hyrcana (431.66±1.05μm). Anatomical characters are effective and important in species segregation. Therefore, anatomical characteristics in different plant species will be useful in terms of taxonomic classification.     Manuscript profile
      • Open Access Article

        2 - First report of pear trees buds and twigs blight caused by Phoma glomerata in Iran
        Parsa Teymuri sayed vahid Alavi
        Due to the observation of a disease symptoms resembling fire blight in pear orchards of Mazandaran province, sampling of the symptoms of blight of buds took place through winter 2018-spring 2019. Samples from infected bud and blighted twigs were cut into 2- to 3-mm piec More
        Due to the observation of a disease symptoms resembling fire blight in pear orchards of Mazandaran province, sampling of the symptoms of blight of buds took place through winter 2018-spring 2019. Samples from infected bud and blighted twigs were cut into 2- to 3-mm pieces, surface of the leaves was sterilized by 75% ethanol for 10 s followed by drowning 3 min in 0.5% sodium hypochlorite, rinsed three times with sterile distilled water, and cultured on water agar (WA) and potato dextrose agar (PDA) with 1.5% streptomycin and tetracycline. Petri dishes were incubated at 25°C. The cultures were initially pale brown and turned dark green with age. Embedded pycnidia were generally formed after 5 days. The pycnidia were agglutinating, globose to subglobose. Hyphal tip from the growing edge of colonies cultured for 3 days at 25°C was transferred to PDA to obtain pure cultures. The colonies were whitish initially and then became olive green to dark brown. Conidia were long, ellipsoid, single-celled, and hyaline or slightly pigmented. The fungus was identified as Phoma glomerata morphologicaly (Boerema et al., 2004). Koch's postulates was done for pathogenicity test. Symptom-free one year old plants and branches were inoculated with spore suspension (1×106 spores/ml). Sterile water was sprayed on another set of plants as non-inoculated control. Each inoculated branch was wrapped in a plastic bag and maintained in a greenhouse at two temperature ranges of 15 ± 2°C and 23± 2°C with 90% rational humidity. After 10 days, symptoms similar to references (Chohan and Chand, 1980) were observed and the same fungus (P. glomerata) was re-isolated. The species of the fungus was confirmed by extraction of genomic DNA from a single spore isolate (Amirdehi et al., 2017 and Doyle and Doyle, 1987). Then, ITS1, 5.8S, ITS2 were amplified with universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') and maintained at 95°C for 3 min. Thirty eight cycles of PCR were performed by heating at 94°C for 1 min, 55°C for 1 min, and 72°C for 1 min, followed by a final period at 72°C for 10 min (White et al., 1990). The amplicon was sequenced and analyzed using BLAST software, and showed a homology of 99% with a corresponding sequence of Phoma glomerata (Corda) Wollenw & Hochapfel. The fungus was reported by Mathur (1979) on Ficus elastica for the first time from India. The fungus was also reported with symptoms of wilting and burning of the lateral buds of the pear (Pyrus communis) for the first time by Chohan and Chand (1980) from India. In Iran Phoma glomerata was reported from wheat Triticum aestivum L. and cucumber Cucumis sativus L. (Safaee et al., 2000; Hatami et al., 2008), Ficus elastica (Aghapour et al., 2009), Mandarin (Rostami et al., 2011), Rosemary (Moshrefi et al., 2015). Nevertheless, this species has not been observed on P. communis from Iran. This is the first report of bud and twig blight disease of Pear (P. communis) in Iran caused by the fungus Phoma glomerata.   Manuscript profile
      • Open Access Article

        3 - Investigating and comparing the diversity of vegetative and reproductive traits in some Pyrus genus (Pyrus L.) cultivars in West Azarbaijan province
        Hediyeh Karimi Abbas Ali Dehpour joybari Eslami Jadidi
        خصوصیات رویشی وزایشی4گونه و 23رقم گونهPyrus communisدر مناطق مختلف استان آذربایجان غربی براساس دستورالعمل ملی آزمونهای تمایز،یکنواختی وپایداری ارزیابی شد. دربین صفات رویشی موردنظر5صفت به شکل درخت،2 صفت به شاخه های یکساله و7صفت به برگ تعلق داشتند.8صفت ازبین صفات رویشی(رن More
        خصوصیات رویشی وزایشی4گونه و 23رقم گونهPyrus communisدر مناطق مختلف استان آذربایجان غربی براساس دستورالعمل ملی آزمونهای تمایز،یکنواختی وپایداری ارزیابی شد. دربین صفات رویشی موردنظر5صفت به شکل درخت،2 صفت به شاخه های یکساله و7صفت به برگ تعلق داشتند.8صفت ازبین صفات رویشی(رنگ غالب شاخه ها،تراکم کرک روی شاخه،قدرت رشد رویشی درخت،شکل قائده،طول و عرض برگ،عمق برش حاشیه وشکل انتهای برش حاشیه) دارای تنوع بالا بیشتروقادربه تمایزگونه ها وارقام بودند .دربین صفات عمومی درخت،شاخص ترین آن قدرت رشد درخت بودکه در4گونه 7رقم زیاد،در بقیه ارقام متوسط بود . صفات شاخه یکساله اگرچه ارتباط منطقی بادیگرصفات درخت داشتند،امابه دلیل سطح تنوع پایین موجوددرآنهادرزمره صفات متمایزکننده ارقام نبودند . دربین اندامهای مختلف درخت بیشترین صفات دارای تنوع درمیوه و برگ دیده شد.گروه بندی ارقام باتجزیه خوشه ای با استفاده از کلیه صفات به نحو بهتری قادر به تفکیک آنها براساس شباهتهای ظاهری ویا منشاءبود. دراین گروهبندیPyrus syriaca،باPyrus salicifolia در بین گو نه ها illiam،Decondel وButirra, Decantelیشترین شباهت را باهم داشتنددرگروههای جداگانه طبقه بندی شدند . به طورکلی مجموعه صفات کلیدی دستورالعمل DUSبه خوبی قادر به تفکیک وتمایزارقام بومی گلابی بودند.ریخت شناسی دانه گرده 4گونه از جنس Pyrus L. spp.از استان آذربایجان غربی با استفاده از میکروسکوپ نوری (LM) و میکوسکوپ الکنرونی(SEM) بررسی شد. مطالعات نشان داد دانه های گرده این گونه ها متقارن و سه شیاره بوده و تزیینات دیواره بصورت striate وStriato microreticulateنشان داده شد . تصاویر تمام گونه هاومشخصات ساختار داخلی دانه های گرده این 4 گونه ارائه شده است. Manuscript profile