In the present study, the effect of three types of nitrogen fertilizers under potting and greenhouse cultivation on growth rate, total nitrogen content, protein, free amino acids and leaf and root alkaloids and leaf nitrate reductase activity in American agave cultivar More
In the present study, the effect of three types of nitrogen fertilizers under potting and greenhouse cultivation on growth rate, total nitrogen content, protein, free amino acids and leaf and root alkaloids and leaf nitrate reductase activity in American agave cultivar Marginata was investigated.Fertilizers including urea (46%), NPK compound fertilizer of type 20 20 20, and Dr. Bio biofertilizer (containing Azotobacter) were applied in two concentrations each, and their effects were compared with the effects ofsoil without fertilizer as a control. Increasing fertilizer in all cases, and especially in the case of biofertilizer, reduced the nitrate reductase activity of the leaves and also changed the overall nitrogen content of the root in favor of increasing it in the leaves. Urea increased wet and dry weight, total protein content, free amino acids and leaf and root alkaloids. Biofertilizer significantly increased fresh and dry weight and leaf protein content, fresh weight and content of free amino acids and root protein. This fertilizer had no significant effect on the alkaloid content of leaves and roots. Increasing the NPK fertilizer also increased the fresh and dry weight and free amino acids of the leaves and the content of total proteins and alkaloids in the leaves and roots and decreased the fresh weight and free amino acids of the roots.
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In the present study, the effect of nitrogen fertilizers including urea, NPK and Dr. Bio biofertilizer on growth, content of some elements, flavonoids and anthocyanins in the ornamental-medicinal plant Zamioculcas zamiifoliaEngl. was investigated. Plants were planted in More
In the present study, the effect of nitrogen fertilizers including urea, NPK and Dr. Bio biofertilizer on growth, content of some elements, flavonoids and anthocyanins in the ornamental-medicinal plant Zamioculcas zamiifoliaEngl. was investigated. Plants were planted in pots and in random blocks in the greenhouse of Farhikhtegan of Islamic Azad University of Gorgan under the influence of different concentrations of fertilizer at the levels of 0, 2 and 4 g/l. Based on the results, urea and biofertilizer treatments had more significant effects on various aspects of growth such as fresh weight and dry weight of roots and leaves than NPK fertilizer. Although the flavonoid content of leaves was not affected by fertilizer, but biofertilizer had a significant positive effect on the anthocyanin content of them. Also, concentration 2 g/l of NPK or urea had a negative effect and concentration 4 g/l of urea had a positive effect on the total anthocyanin content of leaves. All treatments had a positive effect on increasing the nitrogen content of roots. Total nitrogen of leaves was not affected by biofertilizer, but showed some increases in other treatments. Both biofertilizer and urea showed positive and significant effects on increasing nitrate reductase activity of leaves. The effect of NPK fertilizer on this enzymatic activity was increased at a concentration of 4 g/l but decreased at a concentration of 2 g/l. All treatments showed significant effects in reducing phosphorus and potassium of leaves and instead increasing them in the roots. Both biofertilizer and NPK had significantly decreased iron content of leaves and roots. In the case of urea, this decrease was observed only for roots and in treatment 2g/ l, while the treatment 4 g/l increased the iron content in both roots and leaves.
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In this research, the effect of different amounts of ammonium sulphate as sulphate fertilizer in 0, 50, 100, 150, 200 Kg/ha under farm conditions on amount of chlorophyll a, b, carotene and xanthophyll and enzymes activity of catalase, peroxidase and nitrate reductase w More
In this research, the effect of different amounts of ammonium sulphate as sulphate fertilizer in 0, 50, 100, 150, 200 Kg/ha under farm conditions on amount of chlorophyll a, b, carotene and xanthophyll and enzymes activity of catalase, peroxidase and nitrate reductase were evaluated. The results showed that increasing of ammounium sulphate content were ncreased amount of chl a, b, carotene and xanthophyll in leaf of canola significantly. Peroxidase and nitrate reductase activities in root of treated plants increased with increasing of sulphate in soil, while the activity of catalase in two parts of canola (root and leaf) did not have any significantly changes at different contents of sulfur in soils.
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Chlorella vulgaris beijernick was grown in medium BG-11 with 5 concentrations of copper (10, 20, 30, 40, 50μM) without aluminum or with aluminum (300μM) and pH 7.1 for 10 days in 20 hours light and 4 hours darkness. In this study, the effect of different treatment More
Chlorella vulgaris beijernick was grown in medium BG-11 with 5 concentrations of copper (10, 20, 30, 40, 50μM) without aluminum or with aluminum (300μM) and pH 7.1 for 10 days in 20 hours light and 4 hours darkness. In this study, the effect of different treatments on the activity of peroxidase, catalase, Ascorbat peroxidase and superoxid dismotase and nitrate reductase, in vivo, was evaluated. Increase of copper concentration caused increase in activity of antioxidant enzymes. Catalase activity in the presence of aluminum in all copper treatments showed a significant reduction, but peroxidase and Ascorbat peroxidase activities in 20μM treatments decreased significantly when compared with control. The significant decrease in the activity of catalase, peroxidase and Ascorbat peroxidase activity, in 20μM copper with aluminum comparing with 20 μM copper without aluminum was also observed. Results also showed that the effect of different concentrations of copper without aluminum on nitrate reductase activity was not significant and most nitrate reductase activity in treatments with copper and aluminum was observed in 40μM that was significant when compared with 20μM treatment and was not significant when compared with other treatments or control. It is concluded that the presence of aluminum, by increasing some of antioxidant enzymes activities, lead to reduce of copper toxicity and algae growth increases.
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Canola contains allelochemicals that effect on metabolism of weeds and crop plants.The aim of this research was to study the effect of aquatic extract of canola (Brassica napus L. cv Hyola 401) on chlorophyll content in cotyledon, nitrate reductase, catalase and peroxid More
Canola contains allelochemicals that effect on metabolism of weeds and crop plants.The aim of this research was to study the effect of aquatic extract of canola (Brassica napus L. cv Hyola 401) on chlorophyll content in cotyledon, nitrate reductase, catalase and peroxidase activity in root, stem and cotyledon of soybean seedling (Glycine max L. cv Gorgan 3) in Hoagland culture. The seeds of canola (Brassica napus L.cv Hyola 401) was planted in pot and provided aquatic extract of total plant in 5 leaf stage.This extract was added to Hoagland culture and catalase,peroxidase and nitrate reductase activities in leaf, stem and root and chlorophyll a and b content in cotyledons in soybean seedling (Glycine max L.cv Gorgan3) after 9 days were evaluated. The results showed that chlorophylls (a and b) amounts in cotyledons and catalase and peroxidase in root and stem and cotyledon decreased in comparison with to control (Hoagland only) in while nitrate reductase activity reduced in cotyledon and in root and stem increasd.
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The ability of green microalgae, Dunaliella salina for existence, growth, nitrat reductase activity and ammonium release in low light, ambient CO2 and saline condition was studied for a better understanding of it is biotechnological importance. Samples were cultivated i More
The ability of green microalgae, Dunaliella salina for existence, growth, nitrat reductase activity and ammonium release in low light, ambient CO2 and saline condition was studied for a better understanding of it is biotechnological importance. Samples were cultivated in Johnson medium whit NaCl, 7.5, 15, 30 and 50% or without NaCl(control), 30◦C±1◦C, continious white light, 1500 lux, provided by fluorescent lamps and were aerated continiously using aquarium pumps. Based on the results of the present research, the best growth obtained in NaCl 7.5% for along term period, although in short term, a very high growth rate was obtained in NaCl 50% (Saturated salinity). Ammonium release was maximum in NaCl 50% and the nitrate reductase activity was minimum in the same salinity but there was no statistically significant correlation between ammonium release and nitrate reductase activity. The ability of Dunaliella salina in releasing high amount of ammonium and growth in low and high salinity makes it an important candidate as a new biofertilizer.
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In this experiment, we studied the effects of different NaCl solutions on (2.5%, 7.5% and 12.5% NaCl) proliferation, nitrate reductase activity, ammonium release, and protein content in the unicellular, biflagellate, halophilic micro algae, Dunaliella salina. Johns More
In this experiment, we studied the effects of different NaCl solutions on (2.5%, 7.5% and 12.5% NaCl) proliferation, nitrate reductase activity, ammonium release, and protein content in the unicellular, biflagellate, halophilic micro algae, Dunaliella salina. Johnson medium with 2.5%, 7.5% and 12.5% NaCl were selected for treatment. Growth condition was maintained in all experiments (light, 5000 Lux, aeration 1hour daily, temperature 25±5◦C,). The microscopic results showed that in the salt solutionsDunaliella salina can proliferate and shows normal morphology and mobility. Based on results of this experiment, optimum proliferation occurred in 7.5% NaCl. Protein content didn’t show significant changes (P
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