Effect of different consentration of NaCl on growth, ammonium releasing,nitrate reductase activity and total protein contents in Dunaliella salina

Subject Areas : Marine Biology

A.S. Borhani Sabzevar ¹ , M.L. Ghorbanli ²

1 - Biology Dep. Faculty of science, Golestan University, Gorgan.
2 - Biology Dep. Faculty of Science Islamic Azad University, Gorgan Branch, Gorgan

Received: 2010-05-21 Accepted : 2011-09-21 Published : 2011-12-21

Keywords: Protein, Ammonium, salt, Dunaliella salina, nitrate reductase activity,

Abstract :

In this experiment, we studied the effects of different NaCl solutions on (2.5%, 7.5% and 12.5% NaCl) proliferation, nitrate reductase activity, ammonium release, and protein content in the unicellular, biflagellate, halophilic micro algae, Dunaliella salina. Johnson medium with 2.5%, 7.5% and 12.5% NaCl were selected for treatment. Growth condition was maintained in all experiments (light, 5000 Lux, aeration 1hour daily, temperature 25±5◦C,). The microscopic results showed that in the salt solutionsDunaliella salina can proliferate and shows normal morphology and mobility. Based on results of this experiment, optimum proliferation occurred in 7.5% NaCl. Protein content didn’t show significant changes (P