Study of growth, ammonium release and nitrate reductase activity of green algae Dunaliella salina in salinity stress, low light and CO2 conditions
Subject Areas : Geneticزهرا Hosseini k 1 , آرین Sateei 2 , Sh . Shokravi 3 , فریبا Amirlatifi 4 , مریم Safaei 5 , عماد Hosseini K 6
1 - - Department of Biology, Islamic Azad University, Gorgan, Iran Member of Young Reaserchers Club
2 - Department of Biology, Islamic Azad University, Gorgan, Iran
3 - Department of Biology, Islamic Azad University, Gorgan, Iran
4 - Department of Biology, Islamic Azad University, Gorgan, Iran Member of Young Reaserchers Club
5 - Department of Biology, Islamic Azad University, Gorgan, Iran Member of Young Reaserchers Club
6 - دانشجوی کارشناسی ارشد پژوهش دانشگاه آزاد رودهن
Keywords: Salinity, Dunaliella, Green algae, Nitrate reductase, Ammonium release,
Abstract :
The ability of green microalgae, Dunaliella salina for existence, growth, nitrat reductase activity and ammonium release in low light, ambient CO2 and saline condition was studied for a better understanding of it is biotechnological importance. Samples were cultivated in Johnson medium whit NaCl, 7.5, 15, 30 and 50% or without NaCl(control), 30◦C±1◦C, continious white light, 1500 lux, provided by fluorescent lamps and were aerated continiously using aquarium pumps. Based on the results of the present research, the best growth obtained in NaCl 7.5% for along term period, although in short term, a very high growth rate was obtained in NaCl 50% (Saturated salinity). Ammonium release was maximum in NaCl 50% and the nitrate reductase activity was minimum in the same salinity but there was no statistically significant correlation between ammonium release and nitrate reductase activity. The ability of Dunaliella salina in releasing high amount of ammonium and growth in low and high salinity makes it an important candidate as a new biofertilizer.
سلطانی، ن.، شکروی، ش. و فتوت احمدی، ع، (1381). جمع آوری، شناسایی و بررسی اکولوژیک جلبک سبزدونالیلا. گزارش پایانی طرح پژوهشکده علوم پایه کاربردی. جهاد دانشگاهی شهید بهشتی.
Ben- Amotz, A. and Avron, M. (1973). The rode of glycerol in the osmotic regulation of the halophilic alga Dunaliella Parva. Plant physiol, 51: 875-878.
Berges, J. A. (1997). Algal nitrate reductases. Exr.J.Phycol, 32: 3-8.
Einspahr, K.J., Peeler, T.C and G.A.Jr. Thompson. (1988). Rapid changes in polyphosphoinositide metabolism associated with the response of Dunaliella salina to hypoosmotic shock. Journal of Biological Chemistry, 263: 5775-5779.
Ernest, I., Deicher, M., Herman, P.M.J. and Vollenzien, U.I.A. (2005). Nitrate and Phosphat effect cultiviability of Cyanobacteria from inverment with low nitriant levels, applied and invoermental microbiology,Vol.71,no.6 pp :3379-3383
Garsia, F., Freile-Pelegrin, Y. and Robledo, D. (2007). Physiologycal characterization of Dunaliella sp. (Chlorophyta, Volvocales from Yucatan, Mexico. Bioresource Technology, 98:1359-1365.
Ginzburg, B.Z. (1978). In Energitics and Structure of Halophilic Microorganisms (Kaplan, S.R. and Ginz-burg, M., eds.), Elsevier/North-Holland Biomedical press. Amesterdam. PP, 543-560
Harrison, W.G. (1976). Nitrate metabolism of the red tide dinoØagellate Gonyaulax polyedra Stein. J. Exp. Mar. Biol. Ecol., 21: 199-209.
Hasegawa, P.M., Bressan, R.A., Zhu, J. and Bohner, H.J. (2000). Plant Cellular an Molecular Responses to high Salinity. Annu Rev Plant Physiol Plant Mol Biol, 51: 463-499.
Hochman, A. (1982). Studies of nitrate reductase in the fresh water dinoØagellate Peridinium cinctum. Arch. Microbio., 133: 62-65.
Johnson, M.K., Johnson, E.J., Mac Elroy, R.D., Speer, H.L. and Bruff, B.S. (1968). Effects of Salt on the halophilic alga Dunaliella Viridis. J Bacteriol, 95: 1461-1468.
Riisgard, H.U. (1981). Cell volume responses in the naked marine flagellate Dunaliella marina transferred from darkness to light of different intensities. Bot.Mar, 24, 657.
Solarzano, L. (1969). Determination of ammonia in natural waters by the phenol-hypochlorite method. Limno oceanogr, 14: 799-801
Sym, G.J. (1984). Optimisation of the in vivo assay conditions for nitrate reductase in barley (Hordeum Vulgar L.V.Igri). J. Sci. Food Agric, 725-730.
Teodoresco, E.C. (1905). Organization et developpement du Dunaliella. Nouveau genre de Volvocacee- Polyblepharidee. Beh Z Bot centralbl. Bd, XV.215-232.
Thiel, T and Protte, B. (2001). Effect on Heterosist differentiation of nitrogen fixation invegtetive cell of the Cyanobacterium Anabeba variable, ATCC 29413, Jornal of bacteriology, vol. 183, NO, 1 pp:280-286.
_||_
سلطانی، ن.، شکروی، ش. و فتوت احمدی، ع، (1381). جمع آوری، شناسایی و بررسی اکولوژیک جلبک سبزدونالیلا. گزارش پایانی طرح پژوهشکده علوم پایه کاربردی. جهاد دانشگاهی شهید بهشتی.
Ben- Amotz, A. and Avron, M. (1973). The rode of glycerol in the osmotic regulation of the halophilic alga Dunaliella Parva. Plant physiol, 51: 875-878.
Berges, J. A. (1997). Algal nitrate reductases. Exr.J.Phycol, 32: 3-8.
Einspahr, K.J., Peeler, T.C and G.A.Jr. Thompson. (1988). Rapid changes in polyphosphoinositide metabolism associated with the response of Dunaliella salina to hypoosmotic shock. Journal of Biological Chemistry, 263: 5775-5779.
Ernest, I., Deicher, M., Herman, P.M.J. and Vollenzien, U.I.A. (2005). Nitrate and Phosphat effect cultiviability of Cyanobacteria from inverment with low nitriant levels, applied and invoermental microbiology,Vol.71,no.6 pp :3379-3383
Garsia, F., Freile-Pelegrin, Y. and Robledo, D. (2007). Physiologycal characterization of Dunaliella sp. (Chlorophyta, Volvocales from Yucatan, Mexico. Bioresource Technology, 98:1359-1365.
Ginzburg, B.Z. (1978). In Energitics and Structure of Halophilic Microorganisms (Kaplan, S.R. and Ginz-burg, M., eds.), Elsevier/North-Holland Biomedical press. Amesterdam. PP, 543-560
Harrison, W.G. (1976). Nitrate metabolism of the red tide dinoØagellate Gonyaulax polyedra Stein. J. Exp. Mar. Biol. Ecol., 21: 199-209.
Hasegawa, P.M., Bressan, R.A., Zhu, J. and Bohner, H.J. (2000). Plant Cellular an Molecular Responses to high Salinity. Annu Rev Plant Physiol Plant Mol Biol, 51: 463-499.
Hochman, A. (1982). Studies of nitrate reductase in the fresh water dinoØagellate Peridinium cinctum. Arch. Microbio., 133: 62-65.
Johnson, M.K., Johnson, E.J., Mac Elroy, R.D., Speer, H.L. and Bruff, B.S. (1968). Effects of Salt on the halophilic alga Dunaliella Viridis. J Bacteriol, 95: 1461-1468.
Riisgard, H.U. (1981). Cell volume responses in the naked marine flagellate Dunaliella marina transferred from darkness to light of different intensities. Bot.Mar, 24, 657.
Solarzano, L. (1969). Determination of ammonia in natural waters by the phenol-hypochlorite method. Limno oceanogr, 14: 799-801
Sym, G.J. (1984). Optimisation of the in vivo assay conditions for nitrate reductase in barley (Hordeum Vulgar L.V.Igri). J. Sci. Food Agric, 725-730.
Teodoresco, E.C. (1905). Organization et developpement du Dunaliella. Nouveau genre de Volvocacee- Polyblepharidee. Beh Z Bot centralbl. Bd, XV.215-232.
Thiel, T and Protte, B. (2001). Effect on Heterosist differentiation of nitrogen fixation invegtetive cell of the Cyanobacterium Anabeba variable, ATCC 29413, Jornal of bacteriology, vol. 183, NO, 1 pp:280-286.
