List of Articles Lung Cancer

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  1 - Assessment of CXCL12 Gene Expression in HumanLung Adenocarcinoma Cell Line A549by Real Time PCR Method
  Rayhaneh Mahbubi Jalil Fallah Mehrabadi Elaheh Ali Asgari
  Inroduction & Objective: Lung cancer is the most common malignancy and the leading cause of cancer related mortality among women and men worldwide, in such a way that the 5-year survival of patients is about 15%. According to the invasive role of CXCL12, challenges in v More

  Inroduction & Objective: Lung cancer is the most common malignancy and the leading cause of cancer related mortality among women and men worldwide, in such a way that the 5-year survival of patients is about 15%. According to the invasive role of CXCL12, challenges in various studies about expression and non-expression of CXCL12 and the presence of metastasis in early stages of lung adenocarcinoma, the A549 cell line is selected in order to investigate CXCL12 gene expression in it.Material and Methods:In this study A549 cell line was cultured in a DMEM medium contain 10% fetal serum bovine and 1% penicillin- streptomycin. RNA extraction was performed from the mentioned cell line and after qualitative and quantitative evaluation of extracted total RNA, cDNA was synthesized. Then CXCL12 expression was measured using Real time PCR.Results: CXCL12 low expression in lung adenocarcinoma A549 Cell line shows that it can be considered as a potential markerfor predictive and therapeutic purposes.Conclusion:CXCLL12 has a low expression in A549 cell line. Manuscript profile
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  2 - Examining GLUT1 mRNA level in tissue samples of non-small cell lung cancer
  Fatemeh Saedvand Mehdi Ebrahimi Shohre Zare Karizi
  10.30495/zisti.2023.1971573.1140
  Introduction: Lung cancer is a type of malignant lung tumor, which is the most important cause of death caused by cancer in humans. GLUT1 is an important transporter of glucose to cells, and its over expression is involved in increasing the metabolism of cancer cells.Ai More

  Introduction: Lung cancer is a type of malignant lung tumor, which is the most important cause of death caused by cancer in humans. GLUT1 is an important transporter of glucose to cells, and its over expression is involved in increasing the metabolism of cancer cells.Aim: Investigating changes in GLUT1 gene expression in non-small cell cancer tissue samples.Materials and Methods: Number of 30 tissue samples of non-small cell lung cancer and 30 tissue samples adjacent to the tumor were obtained from patients referred to Masih Deneshvari Hospital after obtaining consent. After sampling, RNA isolation and cDNA production, GLUT1 and GAPDH mRNA level was measured by Real-Time PCR technique. Statistical analysis of gene expression data was done with t test. Results: In 21 samples (70%), GLUT1 mRNA level in non-small cell lung cancer tissue was increased compared to healthy tissue. In addition, the level of mRNA in tumor tissue increased by 3.4 times compared to normal tissue (P = 0.013).Conclusion: The increase in GLUT1 mRNA level in non-small cell lung carcinoma cells can be a sign of changing the metabolic program of these cells to aerobic glycolysis, which results in tumor expansion and malignancy. Manuscript profile
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  3 - Comparison of Ginger(Zingiber officinale) Hydroalcoholic Extract on the Viability of Cancer Cells and Embryonic Fibroblast Cells
  Elham Hoveizi Tayebeh Mohammadi
  Lung cancer is the most common cause of cancer related death. Ginger is an edible and medicinal plant having important health benefits including anticancer activity. The aim of this study was evaluation and comparison of the effect of hydroalcoholic extract of ginger on More

  Lung cancer is the most common cause of cancer related death. Ginger is an edible and medicinal plant having important health benefits including anticancer activity. The aim of this study was evaluation and comparison of the effect of hydroalcoholic extract of ginger on lung cancer cells and normal mouse embryonic fibroblasts. Human lung cancer cell line A459 and normal mouse embryonic fibroblasts were cultured in vitro and treated with different concentrations of ginger extract for 24, 48 and 72 hours. The MTT assay was used to determine cell viability. Ginger extract in concentrations of 1800 and 2000 µg/ml had killed both of cells at 72 hours after treatment and caused morphological changes in cells which were more obviously in cancer cells. Ginger extract in concentrations of 1000, 1200, 1400, 1800 and 2000 µg/ml killed cancer cells more than embryonic cells(P<0.05). Cytotoxicity effect of ginger on lung cancer cells was more than its effect on normal mouse embryonic fibroblasts and can be regarded as a safe anticancer medicine. Manuscript profile
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  4 - Determination of the level of fgf-9 gene expression in serum samples of patients with lung cancer
  Mehrdad Hashemi fatemeh sadat alavimoghadam elham siasi
  Background: Lung cancer is one of the major causes of cancer deaths. The activation of fgf signals, including fgf-9, is involved in the pathogenesis of several cancers, including lung cancer. Also, fgf-9 may be an indicator for prognosis and is associated with survival More

  Background: Lung cancer is one of the major causes of cancer deaths. The activation of fgf signals, including fgf-9, is involved in the pathogenesis of several cancers, including lung cancer. Also, fgf-9 may be an indicator for prognosis and is associated with survival rates in patients with NSCLC. Therefore, in this study, the rate of fgf-9 gene expression in the serum patients with lung cancer was investigated. Analysis method: In this study, 50 serum samples of healthy subjects and 50 serum samples of NSCLC patients were collected from patients referring to Masih Daneshvari Hospital in Tehran and were collected by questionnaires, individual and clinical data of the subjects. Then, Plasma isolation, RNA extraction, cDNA synthesis, primer design were performed and the rate of changes of fgf-9 expression in the serum of healthy and lung cancer patients was evaluated using Real Time PCR. REST software was used to analyze the results. Findings: In this study, the expression of fgf-9 gene was not significantly different in the serum of patients with the first to third stages of metastasis but in the serum of people with stage IV metastasis the level of expression of fgf-9 gene had a significant reduction of 4.46 (p < 0.05) times than normal samples. Conclusion: According to the results of this study, if these results are confirmed in more samples, the level of the fgf-9 gene expression in the serum of individuals can probably be used in the future to predict the stage of metastasis of lung cancer Manuscript profile
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  5 - Investigation of hydroalcoholic extract effect of Cordia Myxa fruit on the expression of BCL-2 and BAX genes in the apoptotic pathway and cell proliferation in human lung cancer cell line.
  NAHID ASKARI Kian Aghaabbasi elham rezvannejad
  10.30495/jdb.2023.1987391.1368
  Cancer is one of the most important causes of death worldwide. On the other hand, the therapeutic use of medicinal plants has increased during recent years. For this purpose, the aim of the present research was to investigate the effect of a Cordia myxa fruit extract on More

  Cancer is one of the most important causes of death worldwide. On the other hand, the therapeutic use of medicinal plants has increased during recent years. For this purpose, the aim of the present research was to investigate the effect of a Cordia myxa fruit extract on cellular proliferation and apoptosis gene expression (BCL-2 and BAX) in lung cancer cell line (A549). The plant was collected and Hydroalcoholic extract was prepared by macerating method. Various concentrations of Cordia myxa extracts (of 0.25, 0.5, 1, 2, 4 mg/mL) obtained to treat lung cancer and fibroblast cell lines. Cells were examined by MTT-assay and Real Time RT-qPCR to determine the plant extract-induced cytotoxicity in cell lines with and without treatment The results showed that, the concentration of ≥2 mg/ml extract had a significant lethal effect on cancer cells. The expression of BAX gene in the treated cell line increased and the expression of BCL-2 gene decreased compared to the control. The extract of Cordia myxa fruit didn’t show toxicity effects when used in low concentrations. However, this extract demonstrated the lower cytotoxicity against normal fibroblast cells. The combination therapy can be less toxic if both (plant extract and chemotherapeutic agent) use to treat cancers. It can be useful with fewer side effects of chemotherapeutic agents. Manuscript profile
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  6 - The Apoptotic Effects of Testosterone on Lung Cancer (A549) Cells
  Azita Tishehyar Rahim Ahmadi Minoo Mahmoudi Abdolreza Mohamadnia
  Studies have shown that sex steroids affect the proliferation of cancer cells at cellular and molecular levels. The present study investigated the apoptotic effects of testosterone on lung cancer (A549) cells in cell culture medium. In this experimental-laboratory study More

  Studies have shown that sex steroids affect the proliferation of cancer cells at cellular and molecular levels. The present study investigated the apoptotic effects of testosterone on lung cancer (A549) cells in cell culture medium. In this experimental-laboratory study, the cytotoxic effects of testosterone on Hek293 and A549 cells were assayed using MTT method. Real time PCR was used to evaluate the expression levels of Bax and Bcl2 genes in A549 cells exposed to IC50 dose of testosterone. The data were statistically analyzed between groups using one-way ANOVA. Exposure of Hek293 and A549 cells to higher dose of testosterone (1000 microg/ml) of testosterone resulted in significant decrease in cell viability (P<0.001). IC50 dose of testosterone significantly decreased the anti-apoptotic Bcl-2 gene expression level (P<0.001) in A549 cells, however, did not significantly change the expression level of apoptotic Bax gene. The cytotoxic concentration of testosterone induces apoptosis in lung cancer cells by its inhibitory effect on anti-apoptotic Bcl-2 gene expression level. Accordingly, appropriate dose of testosterone has anti-cancer effects against lung cancer cells. Manuscript profile
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  7 - Expression Analysis of Long Non-coding RNAs CCAT1, CCHE1, ANRIL, BANCR, and PICART1 in Lung Cancer
  Nahid Shafaghat Soudeh Ghafouri-Fard Fattah Sotoodehnejadnematalahi
  10.22034/ascij.2021.684785
  Lung cancer is the most common malignancy with the highest mortality worldwide. LncRNAs play complex roles in the initiation and progression of lung cancer, thereby affecting the prognosis of patients. In this study, the expression changes of CCAT1, CCHE1, ANRIL, BANCR, More

  Lung cancer is the most common malignancy with the highest mortality worldwide. LncRNAs play complex roles in the initiation and progression of lung cancer, thereby affecting the prognosis of patients. In this study, the expression changes of CCAT1, CCHE1, ANRIL, BANCR, and PICART1 lncRNAs in tumor tissues compared to normal tissues adjacent to the tumor were investigated and their potential as biomarkers for the diagnosis and prognosis of lung cancer was evaluated. In order to conduct this case-control study, 30 samples of lung cancer tissue and 30 samples of adjacent non-cancerous tissues (ANCTs) were collected and after total RNA extraction and cDNA synthesis, the expression levels of target lncRNAs and β2M reference gene was measured using SYBR Green real-time PCR assay. The statistical analysis of Real-time PCR data showed no significant difference between the expression of these lncRNAs in tumor tissue and normal tissue adjacent to the tumor (P> 0.05). Furthermore, no significant association was observed between the expression level of these lncRNAs and any of the demographic and pathological characteristics of patients (P> 0.05). Based on the findings of this study, it can be concluded that these lncRNAs play no role in lung cancer in Iranian patients. However, to confirm our results, it is necessary to evaluate the expression of these lncRNAs in a larger number of samples.. Manuscript profile