Assessment of CXCL12 Gene Expression in HumanLung Adenocarcinoma Cell Line A549by Real Time PCR Method

Subject Areas :

Rayhaneh Mahbubi ¹ , Jalil Fallah Mehrabadi ² , Elaheh Ali Asgari ³

1 - کارشناسی‌ارشد رشته زیست‌شناسی سلولی و مولکولی، گروه زیست‌شناسی، دانشگاه آزاد اسلامی واحد تهران شرق، تهران. ایران.
2 - دکتری میکروبیولوژی، آزمایشگاه میکروب‌شناسی لیستر، تهران. ایران
3 - دکتری زیست‌شناسی دریا، گروه زیست‌شناسی، دانشگاه آزاد اسلامی واحد تهران شرق، تهران. ایران.

Received: 2016-11-01 Accepted : 2018-08-04 Published : 2016-10-22

Keywords: CXCL12, A549 Cell Line, Real Time PCR, Lung Cancer,

Abstract :

Inroduction & Objective: Lung cancer is the most common malignancy and the leading cause of cancer related mortality among women and men worldwide, in such a way that the 5-year survival of patients is about 15%. According to the invasive role of CXCL12, challenges in various studies about expression and non-expression of CXCL12 and the presence of metastasis in early stages of lung adenocarcinoma, the A549 cell line is selected in order to investigate CXCL12 gene expression in it.Material and Methods:In this study A549 cell line was cultured in a DMEM medium contain 10% fetal serum bovine and 1% penicillin- streptomycin. RNA extraction was performed from the mentioned cell line and after qualitative and quantitative evaluation of extracted total RNA, cDNA was synthesized. Then CXCL12 expression was measured using Real time PCR.Results: CXCL12 low expression in lung adenocarcinoma A549 Cell line shows that it can be considered as a potential markerfor predictive and therapeutic purposes.Conclusion:CXCLL12 has a low expression in A549 cell line.