List of Articles ISSR

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  1 - Valuation of the genetic diversity of the plant plant, Plantago major L., with ISSR molecular markers in Borujerd region
  Narjes Azadbar Zahra sadat Mousavi Khansari Mohammad Mehdi Dehshiri Reza Yari
  Objective: Borujerd region in Lorestan province is one of the best and less known natural habitats of plantago major L. The aim of the current study is to identify its natural habitats in Borujerd region and to evaluate genetic diversity in ecotypes with ISSR molecular More

  Objective: Borujerd region in Lorestan province is one of the best and less known natural habitats of plantago major L. The aim of the current study is to identify its natural habitats in Borujerd region and to evaluate genetic diversity in ecotypes with ISSR molecular markers. Materials and methods: In this research, the genetic diversity of 23 samples from 6 populations of Plantago major L. was studied. The extracted DNA of all 23 samples was done by the kit method. 10 primers with international codes were used for PCR reaction. The presence or absence of the band was stored as a one/zero matrix in the SPSS program. Findings: Out of the total 1632 bands produced, 200 bands (alleles) were polymorphism, which was calculated as 24% of polymorphism. The length of the generated fragments varied between 300 and 1650 bp. Primer P8 produced the most common bands with 61 bands and primer P9 produced the least common bands with 7 bands in the populations. The highest number of amplified bands was 206 bands related to P8 primer and the lowest number of produced bands was 125 bands related to P5 primer. Conclusion: The results show that the ISSR genetic markers, especially the P8 primer, can be used in phylogenetic studies of leek plant. Despite its small area, Borujerd region is suitable for the growth of various ecotypes of the Plantago major L, and therefore the preservation and maintenance of this natural habitat and the propagation of different ecotypes should be considered. Manuscript profile
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  2 - Study on gentic diversity of the honey bee, Apis mellifera L. using microstatellite ISSR markers in three provinces of Iran
  N. Palvaneh E. Soleyman-Nejadian R. Radjabi F. Yaghout nejad
  Microsatellite markers ISSR were used for isolation of Apis mellifera L. from populations of Kurdistan, Khuzestan and Esfahan. DNA was extracted from worker bees by optimized salting method. Reproducing was done by 4 pairs of ISSR Primers. The obtained bands were visibl More

  Microsatellite markers ISSR were used for isolation of Apis mellifera L. from populations of Kurdistan, Khuzestan and Esfahan. DNA was extracted from worker bees by optimized salting method. Reproducing was done by 4 pairs of ISSR Primers. The obtained bands were visible using 2% agaroz gel & satining with ethidium bromaid. The Bands pattern was recorded by presence (1) or absence (0) method. Total of 45 bands were observed which of 84.52 were diffrent.The cluster analysis based on jaccard similarity cofficient indicated the genetic distance. Populations of  Kurdistan & Khuzestan were located in group (I) and Esfahan in group (II). Therefore, populations of  Kurdistan and Khuzestan provinces showed the highest similarity. Manuscript profile
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  3 - Evaluation of genetic diversity in indigenous breeds of Iranian silkworm Bombyx mori using ISSR markers
  M. Zarei E. Sanatgar R. Radjabi H. Shouhani M. Ibrahim Ghaleh Seyedi
  ISSR molecular marker, in order to isolate the Iranian native Bombyx mori silkworm breeds were used. Extracted DNA by using phenol-chloroform was performed. The qualitative and quantitative measurements of extracted DNA and its dilution, was obtained from the bands on 1 More

  ISSR molecular marker, in order to isolate the Iranian native Bombyx mori silkworm breeds were used. Extracted DNA by using phenol-chloroform was performed. The qualitative and quantitative measurements of extracted DNA and its dilution, was obtained from the bands on 1.5% agarose gel and they marked and analyzed. The results showed that the observed bands were between 200-1000 bp and the most bands were observed corresponding to Harati-yellow with 32 bands and Khorasani-lemon had their lowest with 25 bands. Second Primers were the highest number of bands with 43 bands and the fourth primer had the lowest number of bands with 30 bands. Cluster analysis of races, placed them in three main groups. The first groups consisted of Gilani-orange, Harati-yellow and Khorasani-pink, Khorasani-lemon and Baghdadi races placed in seprate groups. In cluster analysis, Gilani-orange showed the most similar to Herati-yellow and this two races with khorasani-pink were the first group. The most genetic similarity were between Gilani-orange and Herati–yellow and the most genetic distance was obtained between Baghdadi and other four races. It is concluded that, ISSR marker can seperate different races of silkworm with different origin very well.there fore to approve the it is suitalle to use more than more than 30 primer for 14 silkworm individuals with 2n= 28 is better. Manuscript profile
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  4 - Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers
  leila fahmideh Lila Jani pour Bahman Fazeli-Nasab
  RAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimu More

  RAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimum number of fragments (7) and  TIBMBC05 had the maximum number of fragments, (22). ISSR marker yielded 79 fragments, where UBC112 and UBC809had the minimum (1) and maximum (15) fragments, respectively. The Diversity Index value of RAPD primers ranged from 0.8 (TIBMBA02) to 0.94 (TIBMBC05) and also Shanoon Diversity and Nei diversity were 0.57 and 0.38, respectively. In ISSR primers The Diversity Index value ranged from 0.0 (UBC112) to 0.92 (UBC809) and also Shanoon Diversity and Nei diversity were 0.57 and 0.39 respectively. According to ISSR and RAPD markers in combination, maximum genetic similarity (0.48) was observed between Kerman and Jandagh Carum carvi while the minimum similarity (0.115) was observed between Kerman and Neishabour Carum carvi with average 0.315.  Also, cluster analysis segregated all populations into 3 groups according to geographical zone. Manuscript profile
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  5 - Variation on biochemical, phytochemical and genetic diversity of fig (ficus carica) from East Azerbaijan province
  akbar ghorbani hamid Hasanpour sezai Ercisli
  Abstract. Fig (Ficus cariaca) is native to western and eastern mediterranean regions such as western and northwestern of Iran. Iran is one of the important fig producers in the world. In this study, biochemical, phycochemistry characteristics and genetic diversity of 38 More

  Abstract. Fig (Ficus cariaca) is native to western and eastern mediterranean regions such as western and northwestern of Iran. Iran is one of the important fig producers in the world. In this study, biochemical, phycochemistry characteristics and genetic diversity of 38 fig genotypes from Arsbaran in East Azerbaijan province in the Horticulture Laboratory of Urmia University in 2015 were investigated. Biochemical results showed that, TSS, pH, TSS/TA, vitamin c, antioxidant capacity, total anthocyanin, total flavonoid and total phenol were significant (p ≤ 0.01). Most of the studied biochemical traits indicated high variation. The results of the molecular data showed that a total of 131 bands were scored, of which 121 bands (92%) were polymorphic. The amount of polymorphic (PIC) information for the primers used varied from 0.22 to 0.47 in ISSR7 and ISSR20 respectively. Also, marker index were between 0.15 to 2.5 in ISSR7 and ISSR20 respectively. Based on the results of cluster analysis, the studied fig genotypes were classified into two main groups, so that the most of collected genotypes from Kalibar belonged to second group. So it could be concluded that geographic location of the studied fig genotypes is the main reason for their separation by this marker. Based on obtained results, the genotypes Kh9 and H17 were suggested for breeding programs. Manuscript profile
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  6 - The Study of some Citrus varieties in Northern of Iran by molecular ISSR markers
  Babak Babakhani yalda Naghashi
  Recognition of genetic diversity and kinship relationships in Citrus is necessary for planning and applying breeding programs, preserving biodiversity, recording new cultivars, and performing molecular studies. In this study, the genetic diversity of 29 varieties of Cit More

  Recognition of genetic diversity and kinship relationships in Citrus is necessary for planning and applying breeding programs, preserving biodiversity, recording new cultivars, and performing molecular studies. In this study, the genetic diversity of 29 varieties of Citrus including: oranges, mandarins, sour orange, pummel, and natural types were investigated by using ISSR marker. In total, 97 bands were obtained using eight primers in which 78 bands were polymorph. The highest and the lowest polymorphism were in ISSR-8 and ISSR-5 with 90% and 73%, respectively. The average polymorphism information content (PIC) was 0.18, which the highest belonged to ISSR-6 and ISSR-8 (0.27) and the lowest belonged to ISSR-1 (0.12). Dendrogram resulting from cluster analysis of UPGMA method with simple matching similarity coefficient classified varieties into five distinct groups. Pummelo was distinguished from the other genotypes in a single cluster. Unshiu mandarin (Sugiyama) was classified into a group and separated from Clemantine mandarin (Nules). All genotypes including Siavaraz 1, Siavaraz 2, Siavaraz 3, Siavaraz 4, natural types, Parson brown orange and Washington navel orange were clustered into the same group and showed high similarity to gather. The studey of molecular marker can provide useful information about the level of polymorphism and variation in citrus fruits which indicating it’s apply in detection of citrus germplasm. Manuscript profile
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  7 - Study on genetic variation of Hyrcanian Calonectria pseudonaviculata (boxwood blight agent) isolates using RAPD and ISSR markers
  Parisa Khazaeli Saeed Rezaee Mansoureh Mirabolfathy Hamidreza Zamanizadeh Hadi Kiadaliri
  Background & Objectives: Boxwood tree is one of the Iranian endemic trees expanded throughout Hyrcanian forests. They are of particular importance among the world's forest reserves. Recently, Calonectria pseudonaviculata has been considered as one of the most import More

  Background & Objectives: Boxwood tree is one of the Iranian endemic trees expanded throughout Hyrcanian forests. They are of particular importance among the world's forest reserves. Recently, Calonectria pseudonaviculata has been considered as one of the most important causes of blight and leaf defoliation of the boxwood in Iran. For the first time, the present study was aimed to evaluate the genetic variation of Hyrcanian C. pseudonaviculata isolates using RAPD and ISSR markers. Material & Methods: In this study, 75 fungal isolates were collected from the infected boxwoods throughout Hyrcanian forests in the North of Iran. The isolates were assessed based on morphology and colony color. Genetic diversity of the isolates was studied using RAPD and ISSR markers. A part of the beta-tubulin gene was sequenced and deposited at NCBI. Phylogenetic analysis was carried out using PAUP* v. 4.0b10. Results: The colony color of isolates on potato dextrose agar (PDA) medium was brown with pale hale, conidia were cylindrical, rounded at both ends, 1-septate, 48- 68 µm. Stipe was long (90-14 µm) and hyaline, with the extension terminating in a broadly ellipsoid papillate vesicle, the widest part above the middle. Phylogenetic tree based on the β-tubulin gene showed that all isolates are placed into the same group. Our results indicated that the population of this fungus has a low genetic diversity in Iran. Conclusion: It seems that the variation of this pathogen is started to change genetically in some areas due to selection pressure phenomenon. Manuscript profile