Evaluation of genetic diversity in indigenous breeds of Iranian silkworm Bombyx mori using ISSR markers

Subject Areas : entomology and othea arthropods

M. Zarei ¹ , E. Sanatgar ² , R. Radjabi ³ , H. Shouhani ⁴ , M. Ibrahim Ghaleh Seyedi ⁵

1 - Entomology Department, Faculty of Agriculture, Islamic Azad University, Arak Branch, Arak, Iran
2 - Entomology Department, Faculty of Agriculture, Islamic Azad University, Arak Branch, Arak, Iran
3 - Plant Protection Department, Faculty of Agriculture, Islamic Azad University, Dezful Branch, Dezful, Iran
4 - Entomology Department, Agriculture College, Islamic Azad University, Jahrom Branch, Jahrom, Iran
5 - Entomology Department, Agriculture College, Islamic Azad University, Jahrom Branch, Jahrom, Iran

Received: 2012-06-30 Accepted : 2012-12-25 Published : 2013-11-22

Keywords:

Abstract :

ISSR molecular marker, in order to isolate the Iranian native Bombyx mori silkworm breeds were used. Extracted DNA by using phenol-chloroform was performed. The qualitative and quantitative measurements of extracted DNA and its dilution, was obtained from the bands on 1.5% agarose gel and they marked and analyzed. The results showed that the observed bands were between 200-1000 bp and the most bands were observed corresponding to Harati-yellow with 32 bands and Khorasani-lemon had their lowest with 25 bands. Second Primers were the highest number of bands with 43 bands and the fourth primer had the lowest number of bands with 30 bands. Cluster analysis of races, placed them in three main groups. The first groups consisted of Gilani-orange, Harati-yellow and Khorasani-pink, Khorasani-lemon and Baghdadi races placed in seprate groups. In cluster analysis, Gilani-orange showed the most similar to Herati-yellow and this two races with khorasani-pink were the first group. The most genetic similarity were between Gilani-orange and Herati–yellow and the most genetic distance was obtained between Baghdadi and other four races. It is concluded that, ISSR marker can seperate different races of silkworm with different origin very well.there fore to approve the it is suitalle to use more than more than 30 primer for 14 silkworm individuals with 2n= 28 is better.