Genetic diversity of some wild and cultivated barleys regarding their reaction to powdery mildew using ISSR and RAPD markers
Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهیM. Ahmadi 1 , A. Fazeli 2 , A. Arminian 3
1 - دانش آموخته کارشناسی، اصلاح نباتات، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران
2 - استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران
3 - استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران
Keywords: Resistance to disease, effective allele, ISSR and RAPD,
Abstract :
Genetic diversity of 34 wild and cultivated barley cultivars along with susceptible cultivar namely Afzal was evaluated regarding their sensitivity and resistance to powdery mildew in a randomly complete design with three replications in greenhouse of Ilam University. Plants were inoculated at anthesis stage with Blumeria graminis f.sp. hordei. When 16 ISSR and 6 RAPD primers were used, 125 and 32 alleles were amplified where 99.27% and 100% of the alleles had polymorphism, respectively. Average expected and observed heterogeneity and average Shanon index in ISSR were calculated to be 0.32, 0.25 and 0.35 and for RAPD were 0.28, 0.31 and 0.42. Cluster analysis by UPGMA method categorized genotypes in five clusters of which groups 1 and 2 were resistant to semi-resistant, group 3 as susceptible, and also group 4 and 5 were moderately susceptible to susceptible ones, respectively.In general, our results indicated that using molecular markers such as RAPD and ISSR can differentiate genotypes into resistant or susceptible groups that would help researchers in breeding programs.
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