The formation of the complex between Pd + 2 ions and doxycycline in DMF was studied using ultraviolet spectroscopy at temperatures [15, 25, 35 and 45 ± 0.1] ° C. The process of forming the complex was optimized in terms of pH, temperature and time. The ratio More
The formation of the complex between Pd + 2 ions and doxycycline in DMF was studied using ultraviolet spectroscopy at temperatures [15, 25, 35 and 45 ± 0.1] ° C. The process of forming the complex was optimized in terms of pH, temperature and time. The ratio of stoichiometry (metal ion / ligand ion) was obtained 1: 1. The formation of the complex and the thermodynamic parameters have been obtained by information on the molar ratio method using the KINFIT program. Also the formation of a complex was used to determine the amount of drug and to determine the optical parameters. The formation of the complex between Pd + 2 ions and doxycycline in DMF was studied using ultraviolet spectroscopy at temperatures [15, 25, 35 and 45 ± 0.1] ° C. The process of forming the complex was optimized in terms of pH, temperature and time. The ratio of stoichiometry (metal ion / ligand ion) was obtained 1: 1. The formation of the complex and the thermodynamic parameters have been obtained by information on the molar ratio method using the KINFIT program. Also the formation of a complex was used to determine the amount of drug and to determine the optical parameters.
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Escherichia coli is one of the most important causes of infection in avian species. To reduce the losses caused by this organism, antimicrobials are widely used in the poultry industry. The aim of this study was to compare pulse and continues administration of doxycycli More
Escherichia coli is one of the most important causes of infection in avian species. To reduce the losses caused by this organism, antimicrobials are widely used in the poultry industry. The aim of this study was to compare pulse and continues administration of doxycycline on the clinical signs, lesions and selected biochemical parameters in colibacillosis infected broilers. The study was performed in a colibacillosis infected broiler farm with two similar houses. In the first group Doxycycline was administrated continuously for 4 days and in the second group it was administrated by pulse method for 4 days and 8 hours daily. After treatment, 20 blood samples were taken from each group, and the amounts of ALT, AST, ALP, total protein and creatinine was evaluated by biochemical tests. Differentiated white blood cell counts were also recorded along with gross lesions and clinical signs. Results indicated that there was a significant difference between the two methods concerning the amounts of AST (p<0.05). Although the amounts of ALT, ALP and creatinine in the continuous group were lower than pulse group, but it was not statistically significant. In the continuous group, WBCs and lymphocytes decreased significantly (p<0.05) while hematocrit, heterophils and eosinophil increased significantly (p<0.05). It was demonstrated that the pulse administration of doxycycline had better outcome in treatment of colibacillosis in comparison to continuous administration.
Conflict of interest: None declared.
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هدف از این مطالعه برآورد اثر آنتی‌بیوتیک دئوکسی‌سایکلین روی توسعه بهاره کلنی‌های زنبور عسل، ترکیب شیمیایی بدن‌های زنبوران کارگر، توسعه غده‌های هیپوفارنژیال و برخی پارامترهای فیزیکوشیمیایی عسل بود. آزمایش در طول تغذیه تحریک‌کننده بهاره در مرکز تحقیقات کشت و کشاورزی، اسمو More
هدف از این مطالعه برآورد اثر آنتی‌بیوتیک دئوکسی‌سایکلین روی توسعه بهاره کلنی‌های زنبور عسل، ترکیب شیمیایی بدن‌های زنبوران کارگر، توسعه غده‌های هیپوفارنژیال و برخی پارامترهای فیزیکوشیمیایی عسل بود. آزمایش در طول تغذیه تحریک‌کننده بهاره در مرکز تحقیقات کشت و کشاورزی، اسمولیان، بلغارستان، انجام شد. دو گروه از کلنی‌های زنبور عسل تشکیل داده شدند-کنترل و آزمایشگاهی. گروه کنترل با محلول شکر (شکر/آب 1:1) و گروه آزمایشگاهی با اضافه کردن آنتی‌بیوتیک دئوکسی‌سایکلین در یک دز 500 میلی‌گرم/لیتر در محلول شکر تغذیه شد. مقادیر قابل توجه پایین‌تر برای استحکام کلنی‌های زنبور عسل و میزان تولید زنبور عسل کارگر تغذیه شده با دئوکسی‌سایکلین نسبت به گروه کنترل بدست آمد. همبستگی‌های قوی بین زنبوران کارگر و مقدار گرده زنبور عسل و مقدار عسل و گرده زنبور عسل در هر دو گروه یافت شد. از این مطالعه، این نتیجه‌گیری شد که توسعه غدد هیپوفارنژیال تحت تأثیر مثبت بعد از بکارگیری آنتی‌بیوتیک دئوکسی‌سایکلین در دز 500 میلی‌گرم/لیتر قرار نگرفت. براساس نتایج بدست آمده هیچ تفاوتی در پارامترهای فیزیکوشیمیایی نمونه‌های عسل از گروه کنترل و آزمایشگاهی وجود ندارد. در تمامی موارد تست برای باقی‌مانده‌های دئوکسی‌سایکلین در نمونه‌های عسل از گروه آزمایشی مرتبط و بالا بود. بنابراین، نویسندگان دئوکسی‌سایکلین را در زنبورداری توصیه نمی‌کنند.
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Background and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver More
Background and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver nanoparticles conjugate against Brucella meltensis 16M. Material and Methods: After preparing the doxycycline-silver nanoparticle conjugate, its antimicrobial activity against Brucella meltensis 16M was determined by Well Diffusion Agar method in Muller Hintone Agar media. Also, Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Doxycycline-silver nanoparticle conjugate was determined by Macrodilution method in Muller Hintone Broth media. Finally, antibacterial effect of the nanoparticle was assayed in animal model. Results: The results showed that Doxycycline-silver nanoparticle conjugate has antimicrobial activity against Brucella melitensis 16M in laboratory condition. In mouse model, the conjugate of Doxycycline-silver nanoparticle could decrease effectively the Brucella melitensis load in liver. Conclusion: This study demonstrated that Doxycycline-silver nanoparticle conjugate has synergistic effect on Brucella melitensis 16M and can be useful in treatment of brucellosis.
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