Abstract In this factorial study in a completely randomized design, the effect of different concentrations of colchicine (0, 15, 30, 45 and 60 mg/L) and different time intervals (12, 24 and 48 hours) on the amount of phenol and lignan production was investigated in the More
Abstract In this factorial study in a completely randomized design, the effect of different concentrations of colchicine (0, 15, 30, 45 and 60 mg/L) and different time intervals (12, 24 and 48 hours) on the amount of phenol and lignan production was investigated in the callus tissue of Linum usitatissimum L. For callus induction, cotyledon explants were cultured on solid MS medium supplemented with 2 mg/l 6-Benzylaminopurine (BAP) and 1 mg/l 1-Naphthaleneacetic acid (NAA).The total phenolic and lignan contents were measured by spectrophotometric method based on the standard curves of gallic acid and sesamin at 765 and 288 nm, respectively. The highest amount of fresh and dry weight of callus was related to 45 mg/l colchicine treatment. Maximum and minimum levels of lignin in experimental periods were obtained from 15 mg/l (in average 28.4 mg SE/ g DW) and 30 mg/l (in average 19.75 mg SE/ g DW) colchicine, respectively. According to the results, the highest (164 mg GA/g) and lowest (145.6 mg GA/g) levels of total phenol content were observed in 60 and 30 mg/l colchicine treatments, respectively. The results indicated that the production of valuable secondary metabolites such as phenol and lignan in flax callus were increased under some colchicine treatments.
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In order to study of varying effects of colchicine concentration in 3 levels (100,150 and 200µl) and time effect in 3 levels (1,2 and 3 h) on chromosomal characters between 10 blood cell cultures from Nadjdi goat breed, cytogenetic study was performe More
In order to study of varying effects of colchicine concentration in 3 levels (100,150 and 200µl) and time effect in 3 levels (1,2 and 3 h) on chromosomal characters between 10 blood cell cultures from Nadjdi goat breed, cytogenetic study was performed. The samples were studied for several karyotypic characteristics, including long and short arm length, by which total length of the chromosomes, long arm to short arm and short arm to long arm ratio were calculated. Analysis of variance of mitotic data in factorial experimental design based on completely randomized design, showed highly significant differences between the studied cultures for all of the traits at α=0.01 level. Duncan test were carried out in order to classify the cultures and their chromosomes. All of the ten cultures of the breed had 30 chromosome pairs, Minimum total length of the chromosomes related to 100µl concentration and 1h time exposure and Maximum total length of the chromosome related to 200µl concentration and 2h time exposure. All of the interactions between cultures × chromosomes were non significant.
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Background and objective:As crystals may contribute to inflammation in osteoarthritis (OA), it is hypothesized that colchicines may affect on symptoms of patients with primary knee OA. Our objective was to evaluate the symptomatic benefits of colchicines on pain and fun More
Background and objective:As crystals may contribute to inflammation in osteoarthritis (OA), it is hypothesized that colchicines may affect on symptoms of patients with primary knee OA. Our objective was to evaluate the symptomatic benefits of colchicines on pain and function of patients with primary knee OA.
Methods: There were 180 patients in two groups (90 in colchicines and 90 in control group), enrolled in this study. Those patients with chronic hepatitis, gastrointestinal or hematologic diseases were excluded. At the first, age, sex, pain and knee function were registered. Using visual analogue scale (VAS) for pain and koos ps criteria for knee function. The Case group received 0.5mg colchicines twice a day and both groups received diclophenac in order to use if they had pain. Pain and knee function was measured again after 3 months. For analyzes SPSS v.20 and Statistica v.10 has been used.
Results: Mean age of patients was 60±5.4 years (50-70), and 53.9% were women. There was no significant difference between two groups considering percentage of pain relief (P>0.05). Also there was no significant correlation between pain relief percentage and age, sex, or affected side. But function of knee was significantly higher in case group after 3months (P=0.019). The patients function recovery percentage was statically significant in case group (P=0.001). The relation of function with age (less than 60) P=0.001, sex (women) P=0.004 and affected side (Bilateral) P=0.003 was significant.
Conclusion:More studies are needed in order to routine usage of colchicines in osteoarthritis.
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