The Effects of Varying Concentration of Colchicine on Chromosomal Characters in Nadjdi Goat (CapraHircus)
Subject Areas : Large Animal Diseases
M.S. Malekpoor
1
(Department of Veterinary, Faculty of Agriculture, Islamic Azad University, Shoushtar Branch, Shoushtar, Iran)
A. Bagherpour
2
(Department of Veterinary, Faculty of Agriculture, Islamic Azad University, Shoushtar Branch, Shoushtar, Iran)
Keywords: Goat, Cytogenetic, Colchicine, chromosomal characters,
Abstract :
In order to study of varying effects of colchicine concentration in 3 levels (100,150 and 200µl) and time effect in 3 levels (1,2 and 3 h) on chromosomal characters between 10 blood cell cultures from Nadjdi goat breed, cytogenetic study was performed. The samples were studied for several karyotypic characteristics, including long and short arm length, by which total length of the chromosomes, long arm to short arm and short arm to long arm ratio were calculated. Analysis of variance of mitotic data in factorial experimental design based on completely randomized design, showed highly significant differences between the studied cultures for all of the traits at α=0.01 level. Duncan test were carried out in order to classify the cultures and their chromosomes. All of the ten cultures of the breed had 30 chromosome pairs, Minimum total length of the chromosomes related to 100µl concentration and 1h time exposure and Maximum total length of the chromosome related to 200µl concentration and 2h time exposure. All of the interactions between cultures × chromosomes were non significant.
