Considering that the horse breeding industry is the fourth largest source of income generation in the world, and due to the diversity of species of horses in the world and the necessity of purity determination amongst breeders and horse owners, the importance of laborat More
Considering that the horse breeding industry is the fourth largest source of income generation in the world, and due to the diversity of species of horses in the world and the necessity of purity determination amongst breeders and horse owners, the importance of laboratory research in this regard is justified. According to past research, carried out sporadically in the country and abroad, there is a vacuum in the study of Iranian horses in terms of the diversity of genetic markers such as STR. The present study investigated short tandem repeat (STR) and allele frequency of 50 Arab horses using four loci (VHL20, AHT4, HTG4 and HMS7) recommended by the International Society for Animal Genetics (ISAG). For this purpose, genomic DNA was extracted from whole blood using the Miller procedure and amplified by Multiplex PCR with fluorescent primers. The results indicated the presence of high genetic variability among the population of Arab horses. The number of alleles observed for each locus ranged from 6 to 9 with tAHT4 and VHL20 markers having the highest number of alleles with 9 alleles, and HTG4, with the highest heterozygosity. The HMS7 site had the lowest number of alleles among the sites examined with 7 alleles, and the VHL20 site had the lowest heterozygosity. The results of this study indicate a high frequency of genetic variation among the population of Arab horses.
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This study was conducted evaluate the effect of vitamin E and Selenium injection on serumic levels of thyroid hormones in the Arabian horse. Twelve Arabian stallions of approximately equal age were selected and based on their age (4 and 5 years old), More
This study was conducted evaluate the effect of vitamin E and Selenium injection on serumic levels of thyroid hormones in the Arabian horse. Twelve Arabian stallions of approximately equal age were selected and based on their age (4 and 5 years old), allocated to tow groups each consisting of six animals. In each group, three of the six horses were injected by vitamins E and selenium at a dose rate of 1cc/30 kg intramuscularly every tow days and the other three animals which were chosen as the controls were similarly injected by normal saline solution. Two days alter each injection; blood samples of the horses in each group were collected using venoject from the jugular vein. After separating the blood serum by centrifuging, the levels of T3 and T4 were measured using the ELISA technique. Following the first injection, the serumic levels of thyroid hormones demonstrated a significant increase in comparison with the controls (p<0.05) while after the second injection, the serumic levels of T3 and T4 had decreased to normal values similar to the control groups. However, following the third injection the decreased in T3 and T4 levels was clearly evident and statistically significant (p<0.05). According to the results of the present study, the injection of vitamin E and selenium for more than twice leads to decrease in the seumic levels of T3 and T4 hormones of the horse and this is coupled with reduction of the basic body metabolism which is not beneficial for the animal.
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This study was performed to evaluate the effect of vitamin E and Selenium injection on serumic levels of albumin, protein, calcium, phosphorous, alkaline phosphates, alaninamonitransferas and aspartataminotransferas in the Arabian stallions. In this study 12 Arabian hor More
This study was performed to evaluate the effect of vitamin E and Selenium injection on serumic levels of albumin, protein, calcium, phosphorous, alkaline phosphates, alaninamonitransferas and aspartataminotransferas in the Arabian stallions. In this study 12 Arabian horses were selected in 2 groups of 4 and 5 years old. In each group, horses were allocated to two sub groups of controls and treatment, each of them containing three animals. In the treatment group, 1cc/30 kg vitamin E and selenium was injected intramuscularly in 2 day intervals for 6 days, while in the control group the same amounts of normal saline (0.9%) was injected. Blood samples of all animals were collected from the jugular vein at days 2, 4 and 6 and their sera were harvested by centrifuging and the levels of the parameters were measured using commercial kits and the spectrophotometric method. The results of this study indicated that there was no significant difference on mean serumic albumin, phosphorous, alkaline phosphates, alaninamonitransferas, and spartataminotransferas in the studied animals while mean serumic protein values of 4 year old horses indicated a significant difference following the third vit E – Selenium injection (p<0.05). The levels of calcium showed a significant difference following the second injection in 4 and 5 years old horses (p<0.05). Based on the results, it can be concluded that vit E – Selenium injection could be beneficial for horses without having any side effects on the liver and kidney but alterations of other biochemical and hematologic parameters should also be investigated.
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در این پژوهش کیفیت سم اسبهای Anglo Arabian نه رأس، Haflinger 10 رأس، Maremmano هفت رأس و Monterufoli 15 رأس ارزیابی شد. Anglo Arabian یک نژاد بین­المللی است، دیگر نژادها اسبهای ایتالیایی هستند: Haflinger مشتق شده از Trentino Alto Adige، Maremmano و Monterufoli از More
در این پژوهش کیفیت سم اسبهای Anglo Arabian نه رأس، Haflinger 10 رأس، Maremmano هفت رأس و Monterufoli 15 رأس ارزیابی شد. Anglo Arabian یک نژاد بین­المللی است، دیگر نژادها اسبهای ایتالیایی هستند: Haflinger مشتق شده از Trentino Alto Adige، Maremmano و Monterufoli از Tuscany. نمونههای سم، از پای چپ جلو، به ­منظور ارزیابی خصوصیات فیزیکی-شیمیایی و مواد معدنی موجود در ناخن، در حین پیرایش جمعآوری شدند. دادههای جمعآوری شده به ANOVA، مولفههای اصلی و تجزیه و تحلیل جداکننده معرفی شدند و یک نقشه داغ از فاصله مربع میان محتوای مواد معدنی در سم نژادهای متفاوت انجام شد. نرمترین سم در Monterufoli Pony یافت شد، همچنین دارای بیش­ترین محتوای ماده خشک. بیشترین محتوای Fe، Ni، Pb، Se، مشخصه سم Anglo Arabian بود، اگرچه سم Monterufoli Pony بیشترین محتوای Na را نشان داد. آنالیز چندمتغییره نشان داده است که K، Li، Mn، Na و بیشترین مواد معدنی قابل شناسایی در ناخن سم هستند. در سم Anglo Araian، Haflinger، Maremmano تجمع زیستی بالایی از مواد معدنی وجود داشت، در حالیکه سم Monterufoli Pony به سرعت از طریق مصرف ناخن و فعالیت تنظیم مواد معدنی (Osmoregulation) از بین میرود. تمامی آنالیزهای آماری نشان دادند که سم Monterufoli Pony متفاوت از سم دیگر نژادهای مورد ملاحظه بود. تمامی حیوانات سم با کیفیت خوب مناسب برای تمرین barefoot نشان دادند.
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