The effect of vitamin E and selenium on serum injection on serumic levels of T3 and T4 hormones in the Arabian horse

Subject Areas : Veterinary Clinical Pathology

بهرام  Amouoghli-Tabrizi ¹ (Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.)
A.  Hassanpour ² (Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.)
M.  Khakpour ³ (Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran)
Y.  Mohamadpour-Tanha ⁴ (Graduate of Veterinary Medicine, Islamic Azad University, Tabriz Branch Tabriz, Iran)

Received: 2008-11-13 Accepted : 2009-01-19 Published : 2008-08-22

Keywords: vitamin E and selenium, Arabian horse, Thyroid hormones (T3, T4),

Abstract :

     This study was conducted evaluate the effect of vitamin E and Selenium injection on serumic levels of thyroid hormones in the Arabian horse. Twelve Arabian stallions of approximately equal age were selected and based on their age (4 and 5 years old), allocated to tow groups each consisting of six animals. In each group, three of the six horses were injected by vitamins E and selenium at a dose rate of 1cc/30 kg intramuscularly every tow days and the other three animals which were chosen as the controls were similarly injected by normal saline solution. Two days alter each injection; blood samples of the horses in each group were collected using venoject from the jugular vein. After separating the blood serum by centrifuging, the levels of T3 and T4 were measured using the ELISA technique. Following the first injection, the serumic levels of thyroid hormones demonstrated a significant increase in comparison with the controls (p<0.05) while after the second injection, the serumic levels of T3 and T4 had decreased to normal values similar to the control groups. However, following the third injection the decreased in T3 and T4 levels was clearly evident and statistically significant (p<0.05). According to the results of the present study, the injection of vitamin E and selenium for more than twice leads to decrease in the seumic levels of T3 and T4 hormones of the horse and this is coupled with reduction of the basic body metabolism which is not beneficial for the animal.

References:

1. شهبازی، پ. و ملک‌نیا، ن (1377): بیوشیمی عمومی، انتشارات و چاپ دانشگاه تهران، جلد2 ، صفحات: 83-79.


2. مجابی، ع. (1379): بیوشیمی بالینی دامپزشکی، انتشارات نوربخش، صفحات:  96-89  و180-171.


3. محمدپورتنها، ه. (1385): تعیین تأثیر تزریق ویتامین E و سلنیوم بر سطح سرمی هورمون‌های تیروئیدی T₃ و  T₄ در اسب، پایان‌نامه برای اخذ درجه دکترای دامپزشکی، دانشگاه آزاد اسلامی تبریز، پایان نامه شماره 823.


4. نظیفی، س. (1380): علوم آزمایشگاهی دامپزشکی(آسیب‌شناسی بالینی)، (ترجمه)، تالیف: جی آر دانکن و همکاران، انتشارات دانشگاه شیراز، چاپ اول، صفحات: 329-281، 380 و407 – 399.


5. Albanes, D. and Heinone, O.P.T. (1996): Alpha-tocopherol and beta-carotene supplements and lung cancer incidence in the alpha-tocopherol, beta-carotene cancer prevention study: effects of base-line characteristics and study compliance. J. Natl. Cancer Inst., 88: 1560-70.


6. Allen, A.L. (1995): Hyperplasia of the thyroid gland and musculoskeletal deformities in two equine abortions, Can. Vet. J., 36: 234-236.


7. Arthur, J.R., Morrice, P.C. and Beckett, G.J. (1988): Thyroid hormone concentrations in selenium deficient and selenium sufficient cattle. Rs. Vet. Sci., 45: 122-123.


8. Bayly, W., Andrea, R. and Smith, B. (1996): Thyroid hormone concentrations in racing thoroughbreds. Pferdeheilkunde, 4: 534-538.


9. Daniela, E., Gerhard, F., Ingrid, H. and Hans, P.S. (2001): Effects of feeding high dosages of vitamin E to laying hens on thyroid hormone concentrations of hatching chicks. J. Exper. Zoology, 290(1): 41- 48.


10. Dargatz, D.A. and Ross, P.F. (1996): Blood selenium concentrations in cows and heifers on 253 cow-calf operations in 18 states. J. Anim. Sci., 74: 2891-2895.


11. Enjabert, F., Lebreton, P., Salat, O. and Schelcher, F. (1999): Effects of pre-or postpartum selenium supplementation on selenium status in beef cows and their calves. J. Anim. Sci., 77: 223-229.


12. Gunter, S.A., Beck, P.A. and Phillips, J.K. (2003): Effects of supplementary selenium source on the performance and blood measurements in beef cows and their calves. J. Anim. Sci., 81: 856-864.


13. Hill, G.M., Link, J.E., Meyer, L. and Fritsche, K.L. (1999): Effect of vitamin E and selenium on iron utilization in neonatal pigs. J. Anim. Sci., 77: 1762-1768.


14. Lavlata, P., Podhorsk, A., Pechova, A. and Dvoakr, F. (2004): Influence of parenteral administration of selenium and vitamin E during pregnancy on selected metabolic parameters and colostrums quality in dairy cows at parturition. Vet. Med. Czech, 49(5): 149–155.


15. Mahan, D.C., Cline, T.R. and Richert, B. (1999): Effects of dietary levels of selenium- enriched yeast and sodium selenite as selenium sources fed to growing – finishing pigs on performance, tissue selenium, serum glutathione peroxides activity, carcass characteristics, and loin quality. J. Anim. Sci., 77: 2172-2179.


16. Mahan, D.C. and Parrett, N.A. (1996): Evaluating the efficacy selenium- enriched yeast and sodium selenite on tissue selenium retention and serum glutathione peroxidase activity in grower and finisher swine. J. Anim. Sci., 74: 2967-2974.


17. Naziroglu, M. and Çay, M. (1998): Effects of selenium and vitamin E supplementation on concentrations of plasma thyroid hormones in lambs. Turk. J. Vet. Anim. Sci., 22: 157-160.


18. Nussbaum, A., Schiessler, G., Hammon, H.M. and Blum, J.W. (2002): Growth performance and metabolic and endocrine traits in calves pair-fed by bucket or by automate starting in the neonatal period. J. Anim. Sci., 80: 1545-1555.


19. Podoll, K.L., Bernard, J.B., Ullery, D.E.De., Bar, S.R., Ku, P.K. and Magee, W.T. (1992): Dietary selenate versus selenite for cattle, sheep and horses. J. Anim. Sci., 70: 1965-1970.


20. Sahin, K., Kucuk, O., Sahin, N. and Sari M. (2002): Effects of vitamin C and vitamin E on lipid peroxidation status, serum hormone, metabolite, and mineral concentrations of Japanese quails reared under heat stress. Int. J. Vitam. Nutr. Res., 72(2): 91-100.


21. Stephen, M., Reed, W., Bayly, M. and Debra, C. (2004): Equine Internal Medicine, Mosby, London, pp: 385-415.


22. Symoonds, M.E. (1995): Thyroid hormones and nutrient supplementations in pregnancy. Equine Vet. Educ., 7: 246-248.