Salmonella is one of the leading causes of foodborne illness and serious health concerns worldwide. The high Prevalence of Salmonella is one of the problems in the livestock industry.
Due to the prevalence of resistance to antibiotics, in this study we evaluated the an More
Salmonella is one of the leading causes of foodborne illness and serious health concerns worldwide. The high Prevalence of Salmonella is one of the problems in the livestock industry.
Due to the prevalence of resistance to antibiotics, in this study we evaluated the antibacterial effects of Yarrow hydroalcoholic extract on Salmonella with antibiotic resistance gene isolated from diarrhea dogs. For this purpose, fecal samples of 83 dogs with diarrhea were immediately cultured by swap in differential media. After confirmation of Salmonella isolates in both molecular and microbial methods, the presence of aminoglycoside resistance genes (aac (3) -IIa and aac (3) -Ia) was investigated. The antibacterial effect of yarrow extract and MIC (minimum inhibitory concentration) was investigated. Finally, the results were analyzed in SPSS 20 software. The results of the present study showed that the prevalence of Salmonella bacteria in the fecal sample of diarrhea dogs was 8.5%.In the MIC study among the measured groups, the lowest inhibitory concentration in mg/ml for strains with aac (3) -Ia, aac (3) -IIa, aac (3) -Ia + aac (3) -IIa and isolates without resistance genes were 78.2±66.3, 46.9±22.1, 62.5 and 93.8±44.2, respectively. No significant differences were observed between these groups (p>0.05). Due to the fact that yarrow has different compounds that can have a strong effect on the inhibition of various microorganisms. Therefore, choosing the right treatment strategies such as the use of medicinal plants like yarrow can play an effective role in reducing the prevalence and epidemic of bacterial diseases.
Manuscript profile
The compostion of the oils from flower and leaf of Achillea wilhelmsii C. Koch were collected during the flowering priod from 15 km away from Esfhan on alt. 1650 m. in July 2003. The essential oils were obtained by different methods of distillation (steam distillation a More
The compostion of the oils from flower and leaf of Achillea wilhelmsii C. Koch were collected during the flowering priod from 15 km away from Esfhan on alt. 1650 m. in July 2003. The essential oils were obtained by different methods of distillation (steam distillation and hydrodistillation), the percentage of oils for flower were 0/17% and 0.25% respectively, and calculated on the dry weight, and analysis by GC and GC/MS. The major constituents determined by hydrodistillation method in flower were p–cymene (33.3%), terpinolene (11.2%) and a-thujene (10.9%) and by steam distillation method in flower were p – cymene (22.1%), germacrene B (11%) and terpinolene (9.8%), respectively.
Manuscript profile
Achillea wilhelmsii C. Koch. belongs to Asteraceae family, is widely found in different regions of Iran and used for treatment of different diseases. In this research, apical shoots of Achillea wilhelmsii at three different developmental stages includingvegetative More
Achillea wilhelmsii C. Koch. belongs to Asteraceae family, is widely found in different regions of Iran and used for treatment of different diseases. In this research, apical shoots of Achillea wilhelmsii at three different developmental stages includingvegetative (May), flowering (June) and end of flowering (July) stages were collected from natural habitat in 2016 from Qushchi region (Urmia) in West Azerbaijan peovince. Essential oil were obtained by water - distillation method (Clevenger apparatus) and were analyzed by GC and GC/MS. The results were showed that at different harvest times, the color, yield and the quality and quantity of essential oils were also different. The camphor (57.5%) and camphene (8.56%) were the main components of plant essential oil at the before flowering stage and their amount were reduced after flowering stage. In contrast, the eucalyptol (13.18%), borneol (18.91%), bicyclo heptanes (0.43%) and thujane (15.35%) had the highest amount at the end of flowering stage. Analysis of results were showed that plant essential oils have active ingredients of different quality and quantity at various growth stages and accordingly will have different pharmaceutical effects.
Manuscript profile
مقدمه و هدف: با افزایش مقاومت عوامل بیماریزا در ماهی نسبت به داورهای شیمیایی، استفاده از گیاهان دارویی با خواص ضد میکروبی ضروری به نظر می­رسد. در این مطالعه ترکیبات شیمیایی بخش­های هوایی اسانس بومادران وحشی پرورش یافته در رشته­کوه­های زاگرس (جنوب غربی ای More
مقدمه و هدف: با افزایش مقاومت عوامل بیماریزا در ماهی نسبت به داورهای شیمیایی، استفاده از گیاهان دارویی با خواص ضد میکروبی ضروری به نظر می­رسد. در این مطالعه ترکیبات شیمیایی بخش­های هوایی اسانس بومادران وحشی پرورش یافته در رشته­کوه­های زاگرس (جنوب غربی ایران) تعیین و خواص ضد میکروبی آن بر روی باکتری­ها و قارچ­های بیماریزای شایع در ماهی مورد بررسی قرار گرفت. روش تحقیق: در این مطالعه، خواص ضد میکروبی اسانس بومادران علیه پنج باکتری شامل استرپتوکوکوس اینیائی، یرسینیا راکری، ویبریو آنگوئیلاروم، آئروموناس هیدروفیلا و سودوموناس آئروجینوزا و چهار قارچ بیماریزای: ساپرولگنیا، فوزاریوم سولانی، کاندیدا آلبیکنز و آسپرژیلوس فلاوس مورد ارزیابی قرار گرفت. همچنین، ترکیبات تشکیل دهنده اسانس بومادران با استفاده از دستگاه گاز کروماتوگرافی- طیف سنج جرمی (GC/MS) تعیین شد. نتایج و بحث: بازده اسانس جمع آوری شده از استان چهارمحال و بختیاری 63/0% بود. مهمترین ترکیبات تشکیل دهنده اسانس بومادران شامل: 8-1 سینئول (2/25%)، کامفور (9/18%)، لینالول (9/6%)، بارنئول (7/5%) و آرتیمزیا (3/4%) بود. همه میکروارگانیسم­های مورد مطالعه نسبت به اسانس بومادران حساسیت بیشتری را نسبت به آنتی بیوتیک­های کنترل نشان دادند. بشترین خواص ضد باکتری و ضدقارچی بومادران به ترتیب علیه باکتری یرسینیا راکری (با قطر هاله مهار رشد 4/25 میلی­متر) و قارچ کاندیدا آلبیکنز (25 میلی­متر) مشاهده شد. این در حالی است که کمترین حساسیت نسبت به باکتری استرپتوکوکوس اینیائی (4/16 میلی­متر) و قارچ آسپرژیلوس فلاوس (14 میلی­متر) مشاهده شد. علاوه بر این، حداقل غلظت مهار کنندگی رشد (MIC) اسانس مورد مطالعه در محدوده 125 تا 800 میکروگرم/ لیتر تعیین شد که قادر به مهار رشد باکتری­ها و قارچ­های بیماریزای منتخب بود. توصیه کاربردی/صنعتی: نتایج این تحقیق نشان دهنده آن است که اسانس بومادران می­تواند به عنوان یک منبع بالقوه جدید و موثرتر از آنتی بیوتیک­های تجاری در صنعت آبزیان مورد استفاده قرار گیرد. هر چند که، انجام مطالعات بیشتر به منظور خالص سازی، جداسازی و تعیین ترکیبات دارای خواص ضد میکروبی اسانس بومادران ضروری به نظر می­رسد.
Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
