Evaluation of Antibacterial Effects of Yarrow Hydroalcoholic Extract on Salmonella with Aminoglycoside Resistance Gene Isolated from Dogs with diarrhea

Subject Areas : Journal of Comparative Pathobiology

ابوالفضل Solyemani asl ¹ , مریم Karimi-Dehkordi ²

1 - D.V.M. Graduate, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received: 2022-09-09 Accepted : 2022-09-09 Published : 2022-02-20

Keywords: Achillea Wilhelmsii, Salmonella, Dog, Diarrhea, Antibiotic, Resistance gene,

Abstract :

Salmonella is one of the leading causes of foodborne illness and serious health concerns worldwide. The high Prevalence of Salmonella is one of the problems in the livestock industry. Due to the prevalence of resistance to antibiotics, in this study we evaluated the antibacterial effects of Yarrow hydroalcoholic extract on Salmonella with antibiotic resistance gene isolated from diarrhea dogs. For this purpose, fecal samples of 83 dogs with diarrhea were immediately cultured by swap in differential media. After confirmation of Salmonella isolates in both molecular and microbial methods, the presence of aminoglycoside resistance genes (aac (3) -IIa and aac (3) -Ia) was investigated. The antibacterial effect of yarrow extract and MIC (minimum inhibitory concentration) was investigated. Finally, the results were analyzed in SPSS 20 software. The results of the present study showed that the prevalence of Salmonella bacteria in the fecal sample of diarrhea dogs was 8.5%.In the MIC study among the measured groups, the lowest inhibitory concentration in mg/ml for strains with aac (3) -Ia, aac (3) -IIa, aac (3) -Ia + aac (3) -IIa and isolates without resistance genes were 78.2±66.3, 46.9±22.1, 62.5 and 93.8±44.2, respectively. No significant differences were observed between these groups (p>0.05). Due to the fact that yarrow has different compounds that can have a strong effect on the inhibition of various microorganisms. Therefore, choosing the right treatment strategies such as the use of medicinal plants like yarrow can play an effective role in reducing the prevalence and epidemic of bacterial diseases.

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