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      • Open Access Article

        1 - Effect of Arbuscular Mycorrhiza Inoculation on Nutrient Uptake and Essential Oil Composition of Dragonhead (Dracocphalum moldavica L.) under Drought Stress
        Gobad Salimi Mohammad Feizian Naser Aliasgharzad
        To study essential oil components of Dracocephalum moldavica in response to mycorrhiza fungus inoculation under drought stress condition, a factorial experiment based on completely randomized design with three replications was carried out under greenhouse condition. The More
        To study essential oil components of Dracocephalum moldavica in response to mycorrhiza fungus inoculation under drought stress condition, a factorial experiment based on completely randomized design with three replications was carried out under greenhouse condition. The factors of the experiment were three levels of drought stresses [control, 0.75 maximum allowable depletion (MAD) and 0.5 MAD] and inoculation with mycorrhiza fungi in two levels (no mycorrhiza inoculation as control and Glomus verciforme inoculation). Essential oil components were recognized via gas chromatography (GC) and gas chromatography-mass spectrometric (GC/MS). The result showed that about 36 components in D. moldavica were identified through inoculation with mycorrhiza under drought stress. Concentration of 13 compounds of these were higher than the rest. Compositions of geranial (18.3%), geraniol (30.98%), geranyl acetate (26.78%) and neral (11.94%) had high quantities in essential oil. Drought stress and mycorrhiza inoculation increased the percentage of major essential oil components. The highest essential oil percentage (1.24%) was obtained by mycorrhiza inoculation treatment and drought stress at 0.75 MAD. Drought stress reduced the uptake of nitrogen, phosphorus and potassium elements, while mycorrhiza inoculation increased their uptake. The highest uptake of potassium was in non-stress and mycorrhiza inoculation conditions. It can be concluded that mycorrhiza inoculation under drought stress increased the uptake of nutrients and essential oil composition of dragonhead. Manuscript profile
      • Open Access Article

        2 - Phytochemical changes of Dracocephalum moldavica L. Essential oil under different salinity stresses and application of humic and ascorbic acid
        rasoul narimani Mohhamad Moghaddam Prof. Abdollah Ghasemi Pirbalouti
          Abstract Salinity is one of the most important ecological stresses which reduces productivity. Humic acid as an organic acid and ascorbate as a strong antioxidant can be effective in improving plant yield under salinity stress.In this study, to investigate the ef More
          Abstract Salinity is one of the most important ecological stresses which reduces productivity. Humic acid as an organic acid and ascorbate as a strong antioxidant can be effective in improving plant yield under salinity stress.In this study, to investigate the effect of ascorbic acid and humic acid on the amount and chemical constituents of essential oil (Dracocephalum moldavica L.) under salinity stress, a factorial experiment in a completely randomized design with three replications was conducted at research greenhouse of faculty of Agriculture, Ferdowsi University of Mashhad, Iran. Treatments included salinity at four levels (0, 50, 100 and 150 mM), humic acid and ascorbic acid at three levels (0, 100 and 200 mg / l).The aerial parts of plant in blooming were collected and were extracted by steam distillation method using Clevenger apparatus and its components were separated and identified by gas chromatography and gas chromatography coupled to mass spectrometry. The essential oil content decreased with increasing salinity stress and application of salt stress moderators (ascorbic acid and humic acid) improved this trait. So that at 150 mM salinityit reached to the lowest level (0.2%) and application of 200 mg / l humic acid increased 51.61% of essential oil compared to control. Most of the essential oil constituents in control treatment (no use of moderators and no salinity conditions) were geranial (36.65%), neral (31.94%), geraniol (15.56%), geranyl acetate (0.66), Trans- 4,2- heptadienal (1/25%), linalool (1.09%), pulegone (0.95%) and verbenol (0.4%) which included 99.5% of the essential oil components respectively by 36.65, 31.94, 15.56, 11.66, 1.25, 1.09, 0.95 and 0.4 percent. In different levels of salinity stress with 100 mg/l of humic acid treatment and application of salt stress moderators (100 and 200 mg/l ascorbic acid and 200 mg/l of humic acid) a large number of combinations was observed compared to control treatment. These includedalpha-pinene, beta-pinene, camphene, camphor, alpha-thujone, nerol and thymol.   Manuscript profile
      • Open Access Article

        3 - تاثیر مراحل مختلف نمو میوه بر کمیت و کیفیت اسانس پوست گیاه (Christm Swingle.) Citrus aurantifolia
        راضیه عبادتی اصفهانی عباس حاجی آخوندی پژمان مرادی
      • Open Access Article

        4 - Effect of spraying iron and zinc sulfate fertilizer on lemon balm (Melisa officinalis L.) medicinal plants essential oil component
        Esfandiar Hassani Moghadam Mozafar Dolatshah Mahdi Shaaban Reza Yarahmadi
        In this research order to study the effect of spraying iron and zinc sulfate fertilizer on lemon balm (Melisa officinalis L.) essential oil component at Khoramabad city climate. A factorial experiment was conducted based on a complete randomized design with three replic More
        In this research order to study the effect of spraying iron and zinc sulfate fertilizer on lemon balm (Melisa officinalis L.) essential oil component at Khoramabad city climate. A factorial experiment was conducted based on a complete randomized design with three replications. Two factors including iron sulfate fertilizer at control, 2 and 5 ppm and zinc sulfate fertilizer at control, 2 and 5 ppm. Essential oils isolated by hydro-distillation with Clevenger apparatus. The essence fraction identified and measurement by GC/MS. The result obtained from this study led to identification 28 fractions on lemon balm volatile oil. In the essential oil of lemon balm jeraniol (66.65%), Trans caryophilen (8.41%), caryophylen oxide (4.99%), verbenol (2.17%), citronellol (1.615), alpha morelol (1.525), germacrin D (1.38%) and alpha cadinol (1.68%) were the major components. Results show under fertilizer treatment no significant observed (P ≤ 0.01) at herbal dry yield. But the amount of essence differences between fertilizer treatmentsandsignificant at (P ≤ 0.01) observed.To increasing the essence amount, 5 ppm zinc sulfate treatment was best treatment. I Manuscript profile
      • Open Access Article

        5 - Investigation the effect of chitosan foliar application on yield and qualitative traits of Lemon balm (Melissa officinalis L.)
        A. Naeimi far L. Pourhosseini M.N. Ilkaee R. Kalhor Monfared
         Biological stimulants can play an important role in increasing the essential oil of medicinalplants, through positive effects on growth and effective substances. In this research, the effectof chitosan foliar application as a biological stimulus on the quantitativ More
         Biological stimulants can play an important role in increasing the essential oil of medicinalplants, through positive effects on growth and effective substances. In this research, the effectof chitosan foliar application as a biological stimulus on the quantitative and qualitative traitsof Lemon balm (Melissa officinalis L.) was investigated. This research was carried out in 2017in the greenhouse of Islamic Azad University, Karaj branch, in three replications. Theexperimental treatments included: chitosan at 4 levels, zero (control), 0.5, 1, 1.5 and 2 gramsper liter. The results show that the effects of chitosan on morphological traits including thelength of the main stem, the number of secondary stems, the diameter of the main stem, thelength of the main root, the dry weight of the stem and root, as well as chlorophyll a andchlorophyll b were significant. The maximum mentioned traits were obtained in the treatmentof 1.5 grams per liter of chitosan. The maximum percentage of essential oil was obtained in thetreatment of 2 g/l and the maximum percentage of geraniol and geranial were obtained inconcentration of 1 g/L  Manuscript profile