• Home
  • پلی اتیلن گلیکول
    • List of Articles پلی اتیلن گلیکول

      • Open Access Article

        1 - Partitioning Studies of Alpha-amylase in Aqueous Two Phase System Composing of Polyethylene Glycol and Tri-Sodium Citrate
        مریم Karimi N. Hadidi Gh. Pazuki Sh. Abdolrahimi
        Introduction: One of the scenario for separation of biotechnology products is the application of aqueous two-phase systems (ATPS) rather than traditional extraction by using solvent. The ATPSs are applicable method for separation and purification of biomolecules mixture More
        Introduction: One of the scenario for separation of biotechnology products is the application of aqueous two-phase systems (ATPS) rather than traditional extraction by using solvent. The ATPSs are applicable method for separation and purification of biomolecules mixture. The ATPS can be applied for protein purification of biomolecules from suspension broth. The goal of the present research is the study of partitioning of -amylase in polymer-salt aqueous two-phase system composed of polyethylene glycol with molecular weight of 1000 and tri-sodium citrate salt as a biocompatible salt. Materials and Methods: The aim of this research is to study partitioning of α-amylase enzyme in an aqueous two phase system. The system consists of polyethylene glycol (PEG) with molecular weight of 1000 and tri-sodium citrate that is a biocompatible salt. Results: The results for partitioning of enzyme in ATPS has been modeled and statistically analyzed; and indicated that the partition coefficient of -amylase in the system has the maximum amount of 2.681. Conclusion: In this research it has been proved that ATPS components concentration has an effect on the enzyme partition coefficient. Using experimental results, -amylase has a great tendency to be placed in the PEG rich phase. Manuscript profile
      • Open Access Article

        2 - Application of spectroscopic methods for structural analysis of monoclinic bismuth vanadate nanoparticles (BiVO4) with different morphologies in the degradation of Rhodamine B dye
        majid mozaffari omid niazmand
        In this Research, BiVO4 nanoparticles using the templates of acrylamide, tryptophan, glycine and polyethylene glycol in same situation of pH and temperature for one pot reaction were prepared. The physicochemical properties of the samples were characterized by diffuse r More
        In this Research, BiVO4 nanoparticles using the templates of acrylamide, tryptophan, glycine and polyethylene glycol in same situation of pH and temperature for one pot reaction were prepared. The physicochemical properties of the samples were characterized by diffuse reflectance spectroscopy (DRS), Raman spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction spectrometer (XRD) techniques. According to the appropriate band gap range from a survey of DRS these compounds suitable for degradation organic pollutants under the terms of the visible light. Rhodamine B as one of the great stability of industrial pollution as well as contaminants was selected. All samples payoff in degradation and discoloration of Rhodamine B (Rh B) showed, but BiVO4 prepared by PEG in about 300 minutes، pH 7 and a concentration of 0.2 grams per liter of photocatalytic, the best performance was in the degradation and decolorization. In the process of photocatalytic degradation of Rhodamine B (Rh B) bismuth vanadate best performance was prepared by polyethylene glycol as a bridging agent, Due to the specific structure and a high level of photocatalytic ability is capable of more than the other compounds. Manuscript profile
      • Open Access Article

        3 - The Effect of Paclitaxel Polyethylene Glycol Solution on Hela Cells
        Maryam Afshar Mahrouz Dezfulian
        Inroduction & ObjectivePaclitaxel (PTX) is one of the most effective chemotherapeutic agents for a wide spectrum of cancers, but its therapeutic benefit is often limited by its poor solubility in aqueous solutions and Cremophor El severe side effects. However, the aim o More
        Inroduction & ObjectivePaclitaxel (PTX) is one of the most effective chemotherapeutic agents for a wide spectrum of cancers, but its therapeutic benefit is often limited by its poor solubility in aqueous solutions and Cremophor El severe side effects. However, the aim of this study is to develop highly water-soluble paclitaxel. For this purpose we prepared a new paclitaxel–poly (ethylene glycol) (PEG) conjugates that were characterized and evaluated for their in vitro cytotoxicity.Materials and Methods:The cell lines used were HeLa and L. Both cell lines were maintained in RPMI 1640 medium containing 10% fetal bowin serum and 1% antibiotics. Various dilutions of PTX-PEG conjugate by physical method were prepared and in vitro cytotoxicity and drug uptake were determined by MTT assay on HeLa and Lcells. Data were expressed as the means of three separate experiments, and were compared by analysis of variance (ANOVA) and Tukey's multiple comparison test of SPSS software.ResultsQuite homogeneous solution was obtained and paclitaxel was completely dissolved. Using MTT assay was determined that a lower dose of paclitaxel nanoparticle have a greater efficacy on cancer cells compared with PTX/ Cremophor EL. (* = P-valueConclusion: The new conjugate formulation exhibited a high efficiency of antitumor activity and low toxicity and can be used as a drug delivery vehicle for cancer therapy.   Manuscript profile
      • Open Access Article

        4 - بررسی تاثیر هورمون‌های گیاهی بر شاخص‌های جوانه‌زنی گیاه کتان(Linum usitatissimum) تحت تنش خشکی
        زهرا مرادیان
      • Open Access Article

        5 - تأثیر تنش های خشکی و شوری بر جوانه زنی بذر، رشد و محتوای پرولین گیاهچه شاهی (Lepidium sativum L.)
        محمد حسین امینی فرد حسن بیات
      • Open Access Article

        6 - تأثیر سطوح مختلف تنش خشکی بر صفات مرتبط با جوانه‌زنی و رشد اولیه گیاهچه گیاه دارویی شوید (Anethum graveolens L.)
        اسماعیل قلی‌نژاد
      • Open Access Article

        7 - بررسی تاثیر هورمون‌های گیاهی بر شاخص‌های جوانه‌زنی گیاه کتان(Linum usitatissimum) تحت تنش خشکی
        زهرا مرادیان
      • Open Access Article

        8 - تأثیر تنش های خشکی و شوری بر جوانه زنی بذر، رشد و محتوای پرولین گیاهچه شاهی (Lepidium sativum L.)
        محمد حسین امینی فرد حسن بیات
      • Open Access Article

        9 - تأثیر سطوح مختلف تنش خشکی بر صفات مرتبط با جوانه‌زنی و رشد اولیه گیاهچه گیاه دارویی شوید (Anethum graveolens L.)
        اسماعیل قلی‌نژاد
      • Open Access Article

        10 - بخش بندی پروتئین خام و تولید گاز در سیلاژهای یونجه عمل‌آوری شده با عصاره محصولات فرعی پسته در شرایط آزمایشگاه
        ا. مختارپور ع.ع. ناصریان ر. ولی‌زاده م. دانش مسگران ف. پورملایی
        به منظور بررسی بخش­های مختلف پروتئین خام و تولید گاز در شرایط آزمایشگاه در سیلاژهای یونجه عمل‌آوری شده با سطوح مختلف عصاره محصولات فرعی پسته و ارزیابی ارزش زیستی تانن­های آن، دو آزمایش انجام شد. محصولات فرعی پسته با نسبت 1:5 (وزن به حجم) به مدت 12 ساعت در آب خیس More
        به منظور بررسی بخش­های مختلف پروتئین خام و تولید گاز در شرایط آزمایشگاه در سیلاژهای یونجه عمل‌آوری شده با سطوح مختلف عصاره محصولات فرعی پسته و ارزیابی ارزش زیستی تانن­های آن، دو آزمایش انجام شد. محصولات فرعی پسته با نسبت 1:5 (وزن به حجم) به مدت 12 ساعت در آب خیسانده شدند. پس از صاف کردن و تغلیظ، عصاره خام به علوفه تازه یونجه اضافه شد تا به ترتیب سطوح 1، 2 و 3 درصد ماده خشک یونجه، تانن به دست آید و سپس به مدت 60 روز سیلو شدند. چهار تیمار به این ترتیب بود؛ سیلاژ یونجه بدون عمل‌آوری با عصاره محصولات فرعی پسته، به عنوان شاهد (T0)، اضافه کردن تانن محصولات فرعی پسته در سطح 1 درصد (T1)، 2 درصد (T2) و 3 درصد ماد خشک (T3). عمل‌آوری سیلاژ یونجه، میزان ترکیبات نیتروژنه غیر پروتئینی را کاهش و بخش B2 را در طی سیلو شدن، افزایش داد. تولید گاز تجمعی پس از 96 ساعت انکوباسیون، قابلیت هضم ماده آلی (OMD) و انرژی قابل متابولیسم (ME) در تیمار T2 و T3 در مقایسه با تیمار شاهد کاهش پیدا کرد. میزان تولید گاز و اسیدهای چرب کوتاه زنجیر (SCFA) در سیلاژهای عمل‌آوری شده در حضور پلی اتیلن گلیکول (PEG) افزایش یافت که نشان می­دهد وجود ترکیبات فنولی و تانن­ها باعث کاهش تولید گاز شد. می­توان نتیجه گرفت که عمل‌آوری سیلاژ یونجه با عصاره محصولات فرعی پسته از طریق ممانعت میکروبی، می­تواند منجر به کاهش هدرروی نیتروژن در طی سیلو شدن و کاهش تجزیه پذیری ماده آلی شود. Manuscript profile
      • Open Access Article

        11 - Effect of the ionic liquid 1-butyl-3-methyl-imidazolium bromide as adjuvant on the formation of {PEG 600-potassium citrate} aqueous biphasic system at different temperatures
        Sholeh Hamzehzadeh Mostafa Abbasi
        Abstract: In biotechnology, separation, extraction, and purification of biomolecules using aqueous biphasic systems, as environmental and economic sustainable alternatives for conventional water-organic solvent extraction techniques, have always been the focus of great More
        Abstract: In biotechnology, separation, extraction, and purification of biomolecules using aqueous biphasic systems, as environmental and economic sustainable alternatives for conventional water-organic solvent extraction techniques, have always been the focus of great attention and examina-tion. One approach proposed by researchers is based on the use of ionic liquids (ILs) as adjuvants in ABS, making the capability of these systems for the extraction of biomolecules to be promoted. In this regard, this work is devoted to study the effect of IL 1-butyl-3-methyl imidazolium bromide ([C4C1im] Br) on the formation of ABS Composed of a polyethylene glycol (PEG) with molecular weight 600 and a biodegradable organic salt potassium citrate. For this purpose, the binodal curves and the liquid-liquid equilibrium (LLE) data of the studied ABS, along with the partition coeffi-cients of [C4C1im]Br were determined at two temperatures of 278.15 K and 318.15 K. The results obtained indicate that the ability of [C4C1im]Br to promote the formation of the studied ABS de-creases with increasing temperature, so that at 318.15 K, the addition of IL makes the formation of two-phase system more difficult. In addition, [C4C1im]Br displays the partition coefficients greater than one for all the compositions and temperatures studied, which increases with increasing TLL at a given temperature. Manuscript profile
      • Open Access Article

        12 - The effects of drought stress on seed germination and some of morphological and physiological traits in Trachyspermum ammi
        امین باقی زاده Malihe Afroushte Baratali Fakheri
        In order to assess the response of ajowan to drought stress that induced by PEG 6000 on germination and growth stages, two separate experiments that in each useed completely randomized design with three replications were done. In first experiment, the response of germin More
        In order to assess the response of ajowan to drought stress that induced by PEG 6000 on germination and growth stages, two separate experiments that in each useed completely randomized design with three replications were done. In first experiment, the response of germination of ajowan seeds was investigated to different levels of drought stress include osmotic potential of zero (control), - 1/5, -2, -2/5 and -3bar that prepared by PEG6000. The second experiment was same first, but in this experiment, morphological and physiological characters of ajowan in different level of drought stress were evaluated. Results of Anova at our data showed that drought stress has significant effect (p≤0/01) on germination characters such as germination percentage, germination rate, height of plumule, length of radicle, dry weight of radicle and plumule. Mean comparison of treatments showed, with increase in drought stress, all the above characters have been reduced. Morphological traits such as leaf area, fresh and dry weight of shoot and root and shoot dry weight ratio to root decreased under drought stress. Also, drought stress had a significant effect on the amount of proline and soluble sugars in the aerial part and root. Comparison of mean of treatments showed that, with increasing stress, the amount of proline and soluble sugars in the shoot and root increased (p≤0/01). The results showed, that germination parameters were reduced by drought stress. Ajowan showed differently responce to tolerate drought stress. Ajowan plant for tolerance to drought stress has different reactions, including reduction of traits such as leaf area and dry and fresh weight of aerial part and root and increase traits such as proline content and soluble sugars. Manuscript profile
      • Open Access Article

        13 - The effects of drought stress on seed germination and some of morphological and physiological traits in Trachyspermum ammi
        A. Baghizadeh M. Afroushte, B. Fakheri
        In order to assess the response of ajowan to drought stress that induced by PEG 6000 on germination and growth stages, two separate experiments that in each useed completely randomized design with three replications were done. In first experiment, the response of germin More
        In order to assess the response of ajowan to drought stress that induced by PEG 6000 on germination and growth stages, two separate experiments that in each useed completely randomized design with three replications were done. In first experiment, the response of germination of ajowan seeds was investigated to different levels of drought stress include osmotic potential of zero (control), - 1.5, -2, -2.5 and -3bar that prepared by PEG6000. The second experiment was same first, but in this experiment, morphological and physiological characters of ajowan in different level of drought stress were evaluated. Results of Anova at our data showed that drought stress has significant effect (p≤0/01) on germination characters such as germination percentage, germination rate, height of plumule, length of radicle, dry weight of radicle and plumule. Mean comparison of treatments showed, with increase in drought stress, all the above characters have been reduced. Morphological traits such as leaf area, fresh and dry weight of shoot and root and shoot dry weight ratio to root decreased under drought stress. Manuscript profile
      • Open Access Article

        14 - In vitro investigation of the GdF3:Bi nanoparticles synthesized via hydrothermal method as the dual MRI-CT contrast agent
        Mohammad Abbasi رضا احمدی Amirhossein Moghanian Aazam Jannati Esfehani
        In the present study, the Bismuth doped GdF3 nanoparticles were synthesized via the hydrothermal method and the effect of temperature, time and NH4F concentration was investigated. The Poly Ethylene Glycol was used as the surfactant. The phases characterization was indu More
        In the present study, the Bismuth doped GdF3 nanoparticles were synthesized via the hydrothermal method and the effect of temperature, time and NH4F concentration was investigated. The Poly Ethylene Glycol was used as the surfactant. The phases characterization was inducted via XRD, FE-SEM and EDS techniques. The in vitro investigation of the samples as the contast agents were performed using MR and CT imaging. the sample synthesized at 180 oC,, 6 hours and the NH4F concentration twice the Stoichiometric concentration that had the semi spherical structure with mean size lower than 100 nm was the suitable sample and the in vitro studies show that the particles act as an excellent CT contrast agent and also as an effective MRI contrast agent at concentrations between 22.5 and 180 mM. Briefly, The use of Bismuth dopant ant GdF3 nanoparticles was successfully performed and The particles can used as the potential MRI-CT contrast agents. Manuscript profile
      • Open Access Article

        15 - Effect of micronutrient elements on germination and seedling characteristics in sugar beet under drought stress conditions
        masomeh nasiri raouf ssharifi
        Seeds that grow in root and stem conditions under drought conditions can be found more quickly and quickly, and in poor environmental conditions, they guarantee higher yields. The experiment was a factorial experiment with completely randomized design with three replica More
        Seeds that grow in root and stem conditions under drought conditions can be found more quickly and quickly, and in poor environmental conditions, they guarantee higher yields. The experiment was a factorial experiment with completely randomized design with three replications. The investigated factors were 8 levels of fertilizer treatment 1. Control (no spraying) 2. Pure iron (2 per thousand) 3. Pure manganese (2 per thousand) 4. Pure boron (2 per thousand) 5. Boron and manganese (4 In thousand) 6. Iron and boron (4 per thousand) 7. Iron and manganese (4 per thousand) 8. Manganese and boron (6,000) and five osmotic potential levels (0, 2-, 4-, 6-, 8-). Polyethylene glycol 6000 was used to create various osmotic potentials. With the occurrence of osmotic stress, germination speed, germination uniformity, germination percentage and traits related to root and shoot dry weight, root length and shoot length decreased significantly. Germination percentage is related to root length. The roots become green before other plant organs come out of the seed. Consequently, they are subjected to environmental stress before other organs. Therefore, root length trait is a suitable criterion for choosing drought tolerance. The highest root length was obtained in manganese and the least in iron treatment with manganese. Figures with more germination percentage, seedlings longer and more dry matter. Manuscript profile
      • Open Access Article

        16 - Effect of temperature, drought and salinity stresses on germination of ten medicinal plant seeds
        Mehrab Yadegari Abdollah Ghasemi
        To study the effects of salinity, temperature and drought stresses on germination indices in seeds of alyssum, dill, borage, marigold, saint john's wort, lemon balm, purslane, feverfew, thyme, and fenugreek, factorial experiments in laboratory conditions were performed More
        To study the effects of salinity, temperature and drought stresses on germination indices in seeds of alyssum, dill, borage, marigold, saint john's wort, lemon balm, purslane, feverfew, thyme, and fenugreek, factorial experiments in laboratory conditions were performed in laboratory conditions in completely randomized design with 4 replication. The treatments contained of salinity at 4 NaCl concentrations (0, 0.25, 0.5, 0.75 mM), Drought (PEG in 0, -0.6, -0.9, -1.2 bar) and Temperature (10/20, 15/25, 20/30, 25/35 °C night/day). The results showed that the treatments had significant effect on germination indices. By comparisons between species, the greatest seed vigour and germination percentage were made in seeds of purslane, then purslane seeds were the most tolerant to drought and salinity and other hand, the seeds of lemon balm and thyme in most of measured characters had the minimum values. By increasing salinity and drought levels, root dry/fresh weight, stem dry/fresh weight, root/stem length and vigour of seeds were decreased in all species. Germination percentage was increased by higher level of temperature but decreased by upper levels of salinity and drought stresses. The highest seed vigour was made in temperature of 20/30°C night/day and no salinity and drought stress (control) and the least related to the temperature degrees of 10/20°C night/day and concentration of 0.75 mM of NaCl and -1.2 bar of PEG.  Then the seed of plants for example purslane can introduce for planting in salinity and drought regions, and seed of plants such as thyme and lemon balm ought not to suggest for sowing in this regions. Manuscript profile